抗体

WAVE3(Scar3)

W0952-01

100微升

516美元

多克隆

domestic rabbit

未共轭

WAVE3(Scar3)

兔

多克隆抗体

抗体

100微升

516美元

IgG

Wiskott-Aldrich syndrome proteins (WASPs) mediate actin dynamics by activating the Arp2/3 actin nucleation complex in response to activated Rho family GTPases. In mammals, five WASP family members have been described. Hematopoietic WASP and ubiquitously expressed N-WASP are autoinhibited in unstimulated cells. Upon stimulation they are activated by cdc42, which relieves the autoinhibition in conjunction with phosphatidyl inositol 4,5-bisphosphate. Three WAVE (Wasf, SCAR) family proteins are similar in sequence to WASP and N-WASP but lack the WASP/N-WASP autoinhibition domains and are indirectly activated by Rac (reviewed in 1). Both WASP and WAVE functions appear to be essential, as knockout of either N-WASP or Scar-2 in mice results in cardiac and neuronal defects and embryonic lethality (2,3). Loss of WASP results in immune system defects and fewer immune cells (4). WAVE-2 (WASF2) is widely distributed, while WAVE-1 and WAVE-3 are strongly expressed in brain (5). WAVE-3 may act as a tumor suppressor in neuroblastoma, a childhood disease of the sympathetic nervous system (6). Increased expression of WAVE-3 is seen in breast cancer, and studies in breast adenocarcinoma cells indicate that WAVE-3 regulates breast cancer progression, invasion and metastasis through the p38 mitogen-activated protein kinase (MAPK) pathway (7,8). Applications: Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilution: Western Blot: 1:1000 Immunoprecipitation: 1:50 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Hu Mk Mo Rt

Synthetic peptide corresponding to the central sequence of human WAVE-3.

Detects endogenous levels of total human WAVE-3 protein. The antibody does not cross-react with WAVE-1 or WAVE-2. Species Crossreactivity: mouse, monkey and rat

Supernatant.

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.

未确定

Product Type: Pab Isotype: IgG Host: rabbit Source: human Concentration: Not determined Form: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. Purity: Supernatant. Immunogen: Synthetic peptide corresponding to the central sequence of human WAVE-3. Specificity: Detects endogenous levels of total human WAVE-3 protein. The antibody does not cross-react with WAVE-1 or WAVE-2. Species Crossreactivity: mouse, monkey and rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

免疫沉淀免疫印迹

-20°C

1. Millard, T.H. et al. (2004) Biochem J. 380, 1-17. 2. Yan, C. et al. (2003) EMBO J. 22, 3602-3612. 3. Snapper, S.B. et al. (2001) Nat. Cell Biol. 3, 897-904. 4. Zhang, J. et al. (1999) J. Exp. Med. 190, 1329-4132. 5. Suetsugu, S. et al. (1999) Biochem. Biophys. Res. Commun. 260, 296-302. 6. Sossey-Alaoui, K. et al. (2002) Oncogene 21, 5967-5974. 7. Sossey-Alaoui, K. et al. (2005) Exp. Cell Res. 308, 135-145. 8. Sossey-Alaoui, K. et al. (2007) Am. J. Pathol. [Epub ahead of print]

https://usbio-images.r.worldssl.net/prodimages/14/W0952-01_1.jpg