抗体

Jagged2(JAG2, HJ2)

J0400-05B

100微升

516美元

单克隆

domestic rabbit

未共轭

7H30(C23D2)

Jagged2(JAG2, HJ2)

兔

单克隆抗体

抗体

100微升

516美元

7H30(C23D2)

IgG

Notch signaling is activated upon engagement of the Notch receptor with its ligands, the DSL (Delta, Serrate, Lag2) proteins of single-pass type I membrane proteins. The DSL proteins contain multiple EGF-like repeats and a DSL domain that is required for binding to Notch (1,2). Five DSL proteins have been identified in mammals: Jagged1, Jagged2, Delta-like 1 (Dll1), Dll3 and Dll4 (3). Ligand binding to the Notch receptor results in two sequential proteolytic cleavages of the receptor by the ADAM protease and the γ-secretase complex. The intracellular domain of Notch is released and then translocates to the nucleus where it activates transcription. Notch ligands may also be processed in a way similiar to Notch, suggesting a bi-directional signaling through receptor-ligand interactions (4-6). Applications: Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilution: Western Blot: 1:1000 incubate membrane with diluted antibody in 5% nonfat dry milk, 1xTBS, 0.1% Tween-20 at 4ºC with gentle shaking overnight. Immunoprecipitation: 1:50 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Hu Rt

Synthetic peptide corresponding to residues surrounding Ala117 of human Jagged2.

Recognizes endogenous levels of total human Jagged2 protein. MW:150kD. Does not crossreact with Jagged1. Species Crossreactivity: rat.

纯化

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, <0.02% sodium azide, 50% glycerol.

未确定

Product Type: Mab Isotype: IgG Clone No: 7H30(C23D2) Host: rabbit Source: human Concentration: Not determined Form: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, <0.02% sodium azide, 50% glycerol. Purity: Purified Immunogen: Synthetic peptide corresponding to residues surrounding Ala117 of human Jagged2. Specificity: Recognizes endogenous levels of total human Jagged2 protein. MW:150kD. Does not crossreact with Jagged1. Species Crossreactivity: rat. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

免疫沉淀免疫印迹

-20°C

1.Wilson, A. and Radtke, F. (2006) FEBS Lett. 580, 2860-2868. 2.Hansson, E.M. et al. (2004) Semin. Cancer Biol. 14, 320-328. 3.Chiba, S. (2006) Stem Cells 24, 2437-2447. 4 .Bland, C.E. et al. (2003) J. Biol. Chem. 278, 13607-13610. 5. Six, E. et al. (2003) Proc. Natl. Acad. Sci. USA 100, 7638-7643. 6. LaVoie, M.J. and Selkoe, D.J. (2003) J. Biol. Chem. 278, 34427-34437.

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