产品简要
公司名称 :
US Biological
产品类型 :
抗体
产品名称 :
IKK beta, phosphorylated (Ser177/181) (IkappaB Kinase beta, IKKb, IKK2)
目录 :
I3000-12
规格 :
100微升
价格 :
566美元
克隆性 :
多克隆
宿主 :
domestic rabbit
共轭标签 :
未共轭
抗原修饰 :
磷酸化
反应物种 :
人类
更多信息或购买 :
产品信息
目录号 :
I3000-12
Product wo Prefix :
IKK beta, phosphorylated (Ser177/181) (IkappaB Kinase beta, IKKb, IKK2)
宿主 :
兔
产品类型 :
多克隆抗体
抗原修饰 :
磷酸化
类别 :
抗体
尺寸1 :
100微升
Size1 List :
566美元
抗体亚型 :
IgG
Desc1 :
The NFkB/Rel transcription factors are present in the cytosol in an inactive state, complexed with the inhibitory IkB proteins. Most agents that activate NFkB do so through a common pathway based on phosphorylation-induced, proteasome-mediated degradation of IkB. The key regulatory step in this pathway involves activation of a high molecular weight IkB kinase (IKK) complex, whose catalysis is generally carried out by three tightly associated IKK subunits. IKKa and IKKb serve as the catalytic subunits of the kinase. IKKg serves as its regulatory subunit. Activation of IKK depends on phosphorylation; serines 177 and 181 in the activation loop of IKKb (176 and 180 in IKKa) are the specific sites whose phosphorylation causes a conformational change that results in kinase activation.
Applications:
Suitable for use in Western Blot. Other applications not tested.
Recommended Dilutions:
Western Blot: 1:1000
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Calc Crossreactivity :
Hu Mk Mo Rt
免疫原 :
Phospho-peptide corresponding to a region surrounding serines 177/181 of IKKb.
特异性 :
Recognizes endogenous levels of human IKKb only when phosphorylated at serines 177/181 or 176/180.
Species Crossreactivity: mouse, rat and monkey
纯度 :
Purified by Protein A and peptide affinity chromatography.
形式 :
Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.
浓度 :
未确定
Desc2 :
Product Type: Pab
Isotype: IgG
Host: rabbit
Source: human
Concentration: Not determined
Form: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.
Purity: Purified by Protein A and peptide affinity chromatography.
Immunogen: Phospho-peptide corresponding to a region surrounding serines 177/181 of IKKb.
Specificity: Recognizes endogenous levels of human IKKb only when phosphorylated at serines 177/181 or 176/180.
Species Crossreactivity: mouse, rat and monkey
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Calc Applications Abbrev :
免疫印迹
储存温度 :
-20°C
参考文献 :
1. Baeuerle, P. A. et al., Science 242:540–546 (1988). 2. Beg, A. A. et al., Genes Dev. 7:2064–2070 (1993). 3. Finco, T. S. et al., Proc. Natl. Acad. Sci. USA 91:11884–11888 (1994). 4. Brown, K. et al., Science 267:1485–1488 (1995). 5. Brockman, J. A. et al., Mol. Cell. Biol. 15:2809–2818 (1995). 6. Traenckner, E. B. et al., EMBO J. 14:2876–2883 (1995). 7. Chen, Z. J. et al. Cell 84:853–862 (1996). 8. Zandi, E. et al., Cell 91:243–252 (1997). 9. Karin, M. et al., Oncogene 18:6867–6874 (1999). 10. DiDonato, J. A. et al., Nature 388:548–554 (1997). 11. Mercurio, F. et al., Science 278:860–866 (1997). 12. Johnson, L. N. et al., Cell 85:149–158 (1996). (13) Delhase, M. et al., Science 284:309–313 (1999).
Picture 1 File Name :
https://usbio-images.r.worldssl.net/prodimages/14/I3000-12_1.jpg
更多信息或购买 :
公司信息
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US Biological
4 Technology Way
Salem, MA01970
Salem, MA01970
tech@usbio.net
https://www.usbio.net800-520-3011 781-639-5092
公司总部: 美国
United States Biological是生命科学领域的知名品牌,致力于用真实、有价值的个性化采购经验降低研究用成本。
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