抗体

GLi1 (Glioma-associated Oncogene, Kruppel (Kr) Zinc Finger Protein)

G2035-50B

100微升

516美元

单克隆

小鼠

未共轭

8A15(L42B10)

GLi1 (Glioma-associated Oncogene, Kruppel (Kr) Zinc Finger Protein)

小鼠

单克隆抗体

抗体

100微升

516美元

8A15(L42B10)

IgG1

GLI was first identified as a gene amplified in a malignant glioma (1) capable of transforming primary cells in cooperation with adenovirus E1A (2). GLI belongs to the Kruppel family of zinc finger proteins that includes three mammalian GLI proteins: GLI1, GLI2 and GLI3 (3). These GLI proteins are similar to the Drosophila homolog Ci (Cubitus interruptus) and function as transcription factors activated by the Hedgehog signaling pathway. Hedgehog signaling plays an important role in animal development and is aberrantly activated in many types of cancers (4,5). Applications: Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilution: Western Blot: 1:1000 Immunoprecipitation: 1:50 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap

Hu

Recombinant protein corresponding to human GLI1.

Recognizes endogenous levels of total human GLI1 protein.

纯化

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, <0.02% sodium azide, 50% glycerol.

未确定

Product Type: Mab Isotype: IgG1 Clone No: 8A15(L42B10) Host: mouse Source: human Concentration: Not determined. Form: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, <0.02% sodium azide, 50% glycerol. Purity: Purified Immunogen: Recombinant protein corresponding to human GLI1. Specificity: Recognizes endogenous levels of total human GLI1 protein. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

免疫沉淀免疫印迹

-20°C

(1) Kinzler, K.W. et al. (1987) Science 236, 70–3. (2) Ruppert, J.M. et al. (1991) Mol Cell Biol 11, 1724–8. (3) Kinzler, K.W. et al. (1988) Nature 332, 371–4. (4) Ingham, P.W. and McMahon, A.P. (2001) Genes Dev 15, 3059–87. (5) McMahon, A.P. et al. (2003) Curr Top Dev Biol 53, 1–114.

https://usbio-images.r.worldssl.net/prodimages/3/G2035-50B_1.jpg