产品简要
公司名称 :
Rockland Immunochemicals
产品类型 :
抗体
产品名称 :
HSF1抗体
目录 :
200-501-F50
规格 :
100微克
克隆性 :
单克隆
宿主 :
大鼠
共轭标签 :
未共轭
克隆名称 :
10H8
反应物种 :
人类, 小鼠, 大鼠, 牛
应用 :
免疫印迹, 酶联免疫吸附测定, 免疫沉淀
更多信息或购买 :
产品信息
目录号 :
200-501-F50
名称 :
抗HSF1(大鼠)单克隆抗体-200-501
显示名称 :
HSF1抗体
应用注释 :
Anti-HSF1 Antibody is suitable for WB, IP, IF microscopy, ELISA, and Gel mobility shift assay. Expect a band approximately ~85kDa protein in unstressed cell lysates, and an ~95kDa protein in heat shocked cell lysates, corresponding to the molecular mass of inactive and active forms of HSF1 on SDS PAGE immunoblots. Specific conditions for reactivity should be optimized by the end user.
缓冲液 :
0.02 M钾磷酸盐, 0.15 M氯化钠, pH值7.2
克隆性 :
单克隆
克隆编号 :
10H8
浓度值 :
1毫克/毫升
浓度定义 :
by UV absorbance at 280 nm
共轭 :
(None)
规格 :
100微克
默认单元 :
微克
ELISA稀释浓度 :
1:200
凝胶迁移稀释 :
User Optimized
免疫荧光显微镜稀释浓度 :
10微克/毫升
免疫沉淀稀释度 :
User Optimized
免疫印迹稀释浓度 :
1微克/毫升
有效期 :
Expiration date is one (1) year from date of opening.
格式 :
IgG1
基因名称 :
HSF1
宿主动物 :
大鼠
一般免责声明 :
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
免疫原 :
HSF1 Antibody was produced in rat by repeated immunizations with purified recombinant HSF1 protein.
包装类型 :
干冰
物理状态 :
Liquid (sterile filtered)
纯度和特异性 :
Anti-HSF1 Antibody was purified by Protein G chromatography. A BLAST analysis was used to suggest cross-reactivity with HSF1 from Human, Mouse, Rat, Bovine, Guinea Pig, Hamster, Monkey, and Rabbit based on 100% homology with the immunizing sequence. Cross-reactivity with HSF1 from other sources has not been determined. Heat Shock research.
物种活性 :
Human, Mouse, Rat, Bovine, Guinea Pig, Hamster, Monkey, Rabbit
稳定剂 :
50%(v/v)甘油
储存 :
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
子种类 :
IgG1
同义词 :
HSTF1, Heat shock factor protein 1, Heat shock transcription factor 1, HSF 1
靶物种 :
人类
背景 :
HSF1, or heat shock factor 1, belongs to a family of Heat Shock transcription factors that activate the transcription of genes encoding products required for protein folding, processing, targeting, degradation, and function. The up-regulation of HSP (heat shock proteins) expression by stressors is achieved at the level of transcription through a heat shock element (HSE) and a transcription factor (HSF). Most HSFs have highly conserved amino acid sequences. On all HSFs there is a DNA binding domain at the Nterminus. Hydrophobic repeats located adjacent to this binding domain are essential for the formation of active trimers. Towards the C-terminal region another short hydrophobic repeat exists, and is thought to be necessary for suppression of trimerization. There are two main heat shock factors, 1 and 2. Mouse HSF1 exists as two isoforms, however in higher eukaryotes HSF1 is found in a diffuse cytoplasmic and nuclear distribution in un-stressed cells. Once exposed to a multitude of stressors, it localizes to discrete nuclear granules within seconds. As it recovers from stress, HSF1 dissipates from these granules to a diffuse nuceloplasmic distribution. HSF2 on the other hand is similar to mouse HSF1, as it exists as two isoforms, the alpha form being more transciptionally active than the smaller beta form. Various experiments have suggested that HFS2 may have roles in differentiation and development.
免疫原类型 :
重组蛋白
低内毒素 :
No
样品尺寸 :
No
应用文本 :
ELISA,Gel Shift,IF Microscopy,Immunoprecipitation,Western Blot,
类别 :
一抗
共轭名称 :
未共轭
UniProt数据库 :
Q00613
NCBI :
NP_005517.1
最初图像名称 :
HSF1免疫荧光
更多信息或购买 :
公司信息
![logo](http://aws.labome.com/img/37.png)
Rockland Immunochemicals
321 Jones Blvd
Pottstown, PA 19464
Pottstown, PA 19464
orders@rockland-inc.com
https://www.rockland.com/800-656-7625
公司总部: 美国
Rockland Immunochemicals, Inc.位于宾夕法尼亚州的费城西部,为基础研究、应用研究及临床研究提供磷酸化位点特异性抗体及抗体相关研究工具。公司的技术平台是由一批经验丰富的科学家建立,利用In vitro及In vivo方法制备并纯化单抗及多抗,这些抗体应用于免疫印迹、ELISA、免疫组化、荧光检测、高通量筛选及诊断试剂盒生产等。常用一抗主要应用于以下研究:Akt通路、载脂蛋白、细胞凋亡/细胞周期、细胞因子、细胞信号、酶类、胞外基质、转录因子及泛素相关领域。