抗体

HEF1抗体

200-301-904

100微克

369.00美元

单克隆

小鼠

未共轭

2G9

人类, 小鼠, 大鼠

免疫印迹, 酶联免疫吸附测定, 免疫沉淀

抗HEF1(氨基酸82-398)(小鼠)单克隆抗体-200-301

HEF1抗体

This monoclonal antibody has been tested for use in western blotting, immunoprecipitation and immunofluorescence.  Specific conditions for reactivity should be optimized by the end user.  Expect bands approximately 115 and 105 in size corresponding to isoforms of HEF1 protein by western blotting in the appropriate cell lysate or extract.  This antibody does not recognize p130Cas.  Sin1 has not been tested.  IF was performed using 4% PFA fixed cells.  This monoclonal antibody mostly detects HEF1 localized at the focal adhesion sites.

0.02 M钾磷酸盐, 0.15 M氯化钠, pH值7.2

单克隆

2G9

1.0毫克/毫升

by UV absorbance at 280 nm

(None)

100微克

微克

1:5, 000 - 1:20, 000

1:500

1:1,000

1:5,000

Expiration date is one (1) year from date of opening.

IgG1

NEDD9

小鼠

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Anti-HEF1 monoclonal antibody was produced by repeated immunizations with a synthetic peptide corresponding to amino acid residues 82-398 of human HEF1 protein (hHEF1, 843 aa, predicted MW 92.8 kDa).

干冰

Liquid (sterile filtered)

0.01%(w/v)叠氮化钠

This Protein A purified antibody is directed against human HEF1 protein. The product was purified from tissue culture supernatant by chromatography.  Reactivity occurs against human, mouse and rat forms of the protein.  Reactivity against multiple isoforms is expected.  Reactivity against homologues from other sources is not known. Specificity was determined by partial epitope mapping.

人类, 小鼠, 大鼠

Store vial at -20° C prior to opening.  Aliquot contents and freeze at -20° C or below for extended storage.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.

IgG1 kappa

mouse anti-hEF1 antibody, mouse anti-NEDD-9 antibody, mouse anti-CASL antibody, Cas like docking antibody, CASL antibody, Crk associated substrate related protein antibody, dJ49G10.2 antibody, dJ761I2.1 antibody, Enhancer of filamentation 1 antibody

人类

HEF1, also known as Enhancer of filamentation 1, CRK-associated substrate-related protein, CAS-L, CasL, p105 and Neural precursor cell expressed developmentally down-regulated 9 is the product of the NEDD9 (CASGL) gene.  HEF1 functions as a docking protein that plays a central coordinating role for tyrosine-kinase-based signaling related to cell adhesion. HEF1 may also function in transmitting growth control signals between focal adhesions at the cell periphery and the mitotic spindle in response to adhesion or growth factor signals initiating cell proliferation.  HEF1 may also play an important role in integrin beta-1 or B cell antigen receptor (BCR) mediated signaling in B- and T-cells.  Integrin beta-1 stimulation leads to recruitment of various proteins including CRK, NCK and SHPTP2 to the tyrosine phosphorylated form.  HEF1 forms a homodimer and can heterodimerize with HLH proteins ID2, E12, E47 and also with p130cas.   HEF1 also forms complexes in vivo with related adhesion focal tyrosine kinase (RAFTK), adapter protein CRKL and LYN kinase and also interacts with MICAL and TXNL4/DIM1.  This protein localizes to both the cell nucleus and the cell periphery and is differently localized in fibroblasts and epithelial cells. In fibroblasts, it is predominantly nuclear and in some cells is present in the Golgi apparatus. In epithelial cells, it is localized predominantly in the cell periphery with particular concentration in lamellipodia, but it is also found in the nucleus.  HEF1 is widely expressed although higher levels are detected in kidney, lung, and placental tissue.  HEF1 is also detected in T-cells, B-cells and diverse cell lines.  HEF1 is activated upon induction of cell growth.  Cell cycle-regulated processing produces four isoforms: p115, p105, p65, and p55.  Isoform p115 arises from p105 phosphorylation and appears later in the cell cycle.  Isoform p55 arises from p105 as a result of cleavage at a caspase cleavage-related site and it appears specifically at mitosis. The p65 isoform is poorly detected.  Isoforms p105 and p115 are predominantly cytoplasmic and associate with focal adhesions while p55 associates with the mitotic spindle.

多肽

No

No

内部

酶联免疫吸附测定, IF显微镜, 免疫沉淀, 免疫印迹

User Optimized

一抗

未共轭

369.00美元

Q14511

5453680

抗HEF1抗体-免疫荧光显微镜