其他

Recombinant Human TNF-alpha (HEK293-expressed) Protein, CF

10291-TA-020

20 ug (also 50 ug, 100 ug, 250 ug, 500 ug, 1 mg)

204美元

10291-TA

10291-TA-020

Recombinant Human TNF-alpha (HEK293-expressed) Protein, CF

The TNF-alpha (HEK293-expressed) Protein from 10291-TA-020 is derived from HEK293-derived Recombinant Human TNF-alpha (HEK293-expressed) Protein, CF. The TNF-alpha (HEK293-expressed) Protein has been validated for the following applications: P01375.

肿瘤坏死因子甲

蛋白和酶

20 ug (also 50 ug, 100 ug, 250 ug, 500 ug, 1 mg)

Lyophilized from a 0.2 µm filtered solution in PBS with Trehalose.

未共轭

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

人类

14-18 kDa, under reducing conditions

17 kDa

Measured in a cytotoxicity assay using L-929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. Matthews, N. and M.L. Neale (1987) in Lymphokines and Interferons, A Practical Approach. Clemens, M.J. et al . (eds): IRL Press. 221. The ED50 for this effect is 20-100 pg/mL.

<0.10 EU per 1 µg of the protein by the LAL method.

P01375

Bioactivity

HEK293-derived Recombinant Human TNF-alpha (HEK293-expressed) Protein, CF

Analyzed by SEC-MALS

204美元

Analyzed by SEC-MALS

APC1 protein, Cachectin, Cachetin, DIF, TNF, TNF, monocyte-derived, TNF-a, TNFA, TNFalpha, TNF-alphacachectin, TNFATNF, macrophage-derived, TNFG1F, TNFSF1A, TNFSF2, TNFSF2TNF superfamily, member 2, tumor necrosis factor, tumor necrosis factor (TNF superfamily, member 2), tumor necrosis factor alpha, Tumor necrosis factor ligand superfamily member 2, tumor necrosis factor-alpha

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.