产品简要
公司名称 :
QED Bioscience
产品类型 :
抗体
产品名称 :
抗BDNF抗体
目录 :
56572
规格 :
100微克
价格 :
436美元
克隆性 :
多克隆
宿主 :
domestic rabbit
共轭标签 :
未共轭
反应物种 :
人类, 小鼠
更多信息或购买 :
图像
图像 1 :
Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-BDNF Polyclonal Antibody (56572). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-BDNF Polyclonal Antibody (56572) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Secreted, Cytoplasm, Membrane-bound vesicle. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) BDNF Antibody (D) Composite.
图像 2 :
Western blot analysis of Human HeLa and HEK293T cell lysates showing detection of ~27.9 Kda BDNF protein using Rabbit Anti-BDNF Polyclonal Antibody (56572). Lane 1: MW Ladder. Lane 2: Human HeLa (20 µg). Lane 3: Human 293T (20 µg). Load: 20 µg. Block: 5% milk + TBST for 1 hour at RT. Primary Antibody: Rabbit Anti-BDNF Polyclonal Antibody (56572) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit: HRP at 1:2000 for 1 hour at RT. Color Development: TMB solution for 12 min at RT. Predicted/Observed Size: ~27.9 Kda.
图像 3 :
Western blot analysis of Mouse Brain showing detection of ~27.9 Kda BDNF protein using Rabbit Anti-BDNF Polyclonal Antibody (56572). Lane 1: MW Ladder. Lane 2: Mouse Brain (20 µg). Load: 20 µg. Block: 5% milk + TBST for 1 hour at RT. Primary Antibody: Rabbit Anti-BDNF Polyclonal Antibody (56572) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit: HRP at 1:2000 for 1 hour at RT. Color Development: TMB solution for 12 min at RT. Predicted/Observed Size: ~27.9 Kda.
产品信息
目录号 :
56572
产品名称 :
抗BDNF抗体
NAME+SKU :
抗BDNF抗体(56572)
产品_TYPE :
抗体
描述 :
抗BDNF兔多克隆抗体
SPECIFICITY SHORT :
BDNF
物种活性 :
人类, 小鼠
靶标名 :
BDNF
UniProt数据库 :
P23560
LOCATION :
Secreted
蛋白名 :
脑源性神经营养因子
别称 :
BDNF, Abrineurin [Cleaved into: BDNF precursor form
基因名称 :
BDNF
克隆性 :
多克隆
形式 :
液体
浓度 :
1.0毫克/毫升
规格1 :
100微克
标价规格1 :
436美元
DISTIRBUTOR价格 :
305.2
SKU号规格1 :
56572
应用 :
免疫印迹, IF
应用INSTRUCTIONS :
Immunoblotting: use at dilution of 1:1,000. A band of ~27-28kDa is detected.
Immunofluorescence: use at dilution of 1:100.
These are recommended working dilutions.
Endusers should determine optimal dilutions for their applications.
宿主 :
兔
格式 :
纯化
纯化 :
Purified by immuno-affinity chromatography on the peptide immunogen.
免疫原 :
合成多肽对应于N末端人的BDNF
研究领域 :
神经科学
配方 :
PBS, pH值7.4, 50% 甘油, 0.09% 叠氮化钠
缓冲液 :
磷酸盐缓冲液
缓冲液pH值 :
pH值7.4
缓冲液抗MICROBIAL :
0.09% 叠氮化钠
缓冲液CRYOPRESERVATIVE :
50% 甘油
靶标背景 :
BDNF acts on neurons of the central nervous system and the peripheral nervous system supporting the survival of existing neurons and stimulating growth and differentiation of new neurons and synapses. In the brain, it is active in the hippocampus, cortex, and basal forebrain, areas vital to learning, memory, and higher thinking. It is also expressed in the retina, motor neurons, kidneys, saliva, and prostate.
FUNCTION :
Important signaling molecule that activates signaling cascades downstream of NTRK2 (PubMed:11152678). During development, promotes the survival and differentiation of selected neuronal populations of the peripheral and central nervous systems. Participates in axonal growth, pathfinding and in the modulation of dendritic growth and morphology. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability. {PubMed:11152678, PubMed:12553913, PubMed:29909994}.; [BDNF precursor form]: Important signaling molecule that activates signaling cascades downstream of NTRK2. Activates signaling cascades via the heterodimeric receptor formed by NGFR and SORCS2 (PubMed:24908487, PubMed:29909994). Signaling via NGFR and SORCS2 plays a role in synaptic plasticity and long-term depression (LTD). Binding to NGFR and SORCS2 promotes neuronal apoptosis. Promotes neuronal growth cone collapse (By similarity). {UniProtKB:P21237, PubMed:24908487, PubMed:29909994}.
稀释INSTRUCTIONS :
Dilute in PBS or medium that is identical to that used in the assay system.
STORAGE空调 :
This antibody is stable for at least one (1) year at -20C.
特异性 :
这抗体识别人类和小鼠BDNF
图像Name_1 :
qed-bioscience-anti-bdnf-antibody-56572-1.png
应用1 :
免疫细胞化学
图像Name_2 :
qed-bioscience-anti-bdnf-antibody-56572-2.png
应用2 :
免疫印迹
图像Name_3 :
qed-bioscience-anti-bdnf-antibody-56572-3.png
应用3 :
免疫印迹
更多信息或购买 :
公司信息
QED Bioscience
10919 Technology Place, Suite C San Diego, CA 92127
info@qedbio.com
http://www.qedbio.com800-929-2114 858-675-2405
公司总部: 美国