抗体

抗细胞骨架锚蛋白B抗体

56518

100微克

436美元

单克隆

小鼠

未共轭

S105-13

人类, 大鼠

抗细胞骨架锚蛋白B抗体

抗细胞骨架锚蛋白B抗体(56518)

抗体

抗细胞骨架锚蛋白B小鼠单克隆抗体

细胞骨架锚蛋白B

人类, 小鼠, 大鼠

细胞骨架锚蛋白B

Q01484

Cytoplasm, cytoskeleton , Membrane , Cytoplasm, myofibril, sarcomere, M line , Apical cell membrane , Cell membrane , Cell junction, synapse, postsynaptic cell membrane , Early endosome , Recycling endosome , Lysosome , Mitochondrion , Cytoplasm, myofibril, sarcomere, Z line , Cell membrane, sarcolemma, T-tubule

细胞骨架锚蛋白2

ANK-2, Ankyrin-B, Brain ankyrin, Non-erythroid ankyrin

ANK2

单克隆

液体

1.0毫克/毫升

100微克

436美元

305.2

56518

免疫印迹, 免疫组化

Immunoblotting: use at 1-2ug/ml. A band of >200kDa is detected. Immunohistochemistry: use at 1-5ug/ml. These are recommended concentrations. Enduser should determine optimal concentrations for their applications. Positive control: rat brain membranes.

小鼠

纯化

经免疫亲和层析纯化

IgG1

S105-13

Synthetic peptide correspond- ing to aa 203-496 of human Ankyrin-B (accession no. NP_001120965.1)

神经科学

PBS, pH 7.4, 50% glycerol, 0.09% sodium azide. Purified by Protein G affinity chromatography.

磷酸盐缓冲液

pH值7.4

0.09% 叠氮化钠

50% 甘油

Ankyrins are a family of adaptor proteins that mediate the attachment of integral membrane proteins to the spectrin-actin based membrane skeleton. Ankyrins have binding sites for the beta subunit of spectrin and for at least 12 families of integral membrane proteins. This linkage is required to maintain the integrity of the plasma membranes and to anchor specific ion channels, ion exchangers, and ion transporters in the plasma membrane.

Plays an essential role in the localization and membrane stabilization of ion transporters and ion channels in several cell types, including cardiomyocytes, as well as in striated muscle cells. In skeletal muscle, required for proper localization of DMD and DCTN4 and for the formation and/or stability of a special subset of microtubules associated with costameres and neuromuscular junctions. In cardiomyocytes, required for coordinate assembly of Na/Ca exchanger, SLC8A1/NCX1, Na/K ATPases ATP1A1 and ATP1A2 and inositol 1,4,5-trisphosphate (InsP3) receptors at sarcoplasmic reticulum/sarcolemma sites. Required for expression and targeting of SPTBN1 in neonatal cardiomyocytes and for the regulation of neonatal cardiomyocyte contraction rate (PubMed:12571597). In the inner segment of rod photoreceptors, required for the coordinated expression of the Na/K ATPase, Na/Ca exchanger and beta-2-spectrin (SPTBN1) (By similarity). Plays a role in endocytosis and intracellular protein transport. Associates with phosphatidylinositol 3-phosphate (PI3P)-positive organelles and binds dynactin to promote long-range motility of cells. Recruits RABGAP1L to (PI3P)-positive early endosomes, where RABGAP1L inactivates RAB22A, and promotes polarized trafficking to the leading edge of the migrating cells. Part of the ANK2/RABGAP1L complex which is required for the polarized recycling of fibronectin receptor ITGA5 ITGB1 to the plasma membrane that enables continuous directional cell migration (By similarity). {UniProtKB:Q8C8R3, PubMed:12571597}.

Dilute in PBS or medium which is identical to that used in the assay system.

This product is stable for at least 1 year at -20C. Freeze in multiple aliquots to avoid repeated freeze-thaw cycles.

这抗体识别人类, 小鼠, 和大鼠细胞骨架锚蛋白B

qed-bioscience-anti-ankyrin-b-antibody-56518-1.png

免疫细胞化学

qed-bioscience-anti-ankyrin-b-antibody-56518-2.png

免疫细胞化学

qed-bioscience-anti-ankyrin-b-antibody-56518-3.png

免疫细胞化学