产品简要
公司名称 :
QED Bioscience
产品类型 :
抗体
产品名称 :
抗VGlut1转运蛋白抗体
目录 :
56514
规格 :
100微克
价格 :
436美元
克隆性 :
单克隆
宿主 :
小鼠
共轭标签 :
未共轭
克隆名称 :
S28-9
反应物种 :
人类, 小鼠, 大鼠
更多信息或购买 :
图像
图像 1 :
Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-VGLUT1 Monoclonal Antibody, Clone S28-9 (56514). Tissue: Differentiated SH-SY5Y. Species: Human. Primary Antibody: Mouse Anti-VGLUT1 Monoclonal Antibody (56514) at 1:100. Secondary Antibody: AlexaFluor 488. Counterstain: phalloidin (Alexa 647, red), beta tubulin (Anti-beta III Tubulin Ab, Alexa 555, magenta) Hoechst (blue). (A) Phalloidin (B) Anti-beta III Tubulin Ab. (C) VGLUT1 Antibody. (D) Hoechst (E) Composite.
图像 2 :
Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-VGLUT1 Monoclonal Antibody, Clone S28-9 (56514). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-VGLUT1 Monoclonal Antibody (56514) at 1:100 for overnight at 4°C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) VGLUT1 Antibody (D) Composite.
图像 3 :
Immunohistochemistry analysis using Mouse Anti-VGLUT1 Monoclonal Antibody, Clone S28-9 (56514). Tissue: spinal cord. Species: Mouse. Fixation: 4% PFA. Primary Antibody: Mouse Anti-VGLUT1 Monoclonal Antibody (56514) at 1:500 for 16 hours at RT. Secondary Antibody: Alexa Fluor 555 Donkey Anti-Mouse (red) at 1:2000 for 2 hours at RT. Counterstain: NeuN neuronal stain (green). Magnification: 20X. Courtesy of: Leilei Wang, Ph.D. UT Southwestern Medical Center at Dallas.
产品信息
目录号 :
56514
产品名称 :
抗VGlut1转运蛋白抗体
NAME+SKU :
抗VGlut1转运蛋白抗体(56514)
产品_TYPE :
抗体
描述 :
抗VGlut1转运蛋白小鼠单克隆抗体
SPECIFICITY SHORT :
VGlut1转运蛋白
物种活性 :
人类, 小鼠, 大鼠
靶标名 :
VGlut1转运蛋白
UniProt数据库 :
Q62634
LOCATION :
Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane , Membrane , Cell junction, synapse, synaptosome
蛋白名 :
Vesicular glutamate transporter 1
别称 :
VGluT1, Brain-specific Na(+-dependent inorganic phosphate cotransporter, Solute carrier family 17 member 7
基因名称 :
Slc17a7
克隆性 :
单克隆
形式 :
液体
浓度 :
1.0毫克/毫升
规格1 :
100微克
标价规格1 :
436美元
DISTIRBUTOR价格 :
305.2
SKU号规格1 :
56514
应用 :
免疫印迹, 免疫组化
应用INSTRUCTIONS :
Immunoblotting: use at 1-2ug/ml. A band of ~52kDa is detected.
Immunohistochemistry: use at 1-5ug/ml.
These are recommended concentrations. Enduser should determine optimal concentrations for their applications.
Positive control: rat brain lysate.
宿主 :
小鼠
格式 :
纯化
纯化 :
经免疫亲和层析纯化
抗体亚型 :
IgG1
克隆编号 :
S28-9
免疫原 :
Fusion protein corresponding to aa 493-560 (cytoplasmic C-terminus) of rat VGlut1 (accession no. NP_446311).
研究领域 :
神经科学
配方 :
PBS, pH 7.4, 50% glycerol, 0.09% sodium azide.
Purified by Protein G affinity chromatography.
缓冲液 :
磷酸盐缓冲液
缓冲液pH值 :
pH值7.4
缓冲液抗MICROBIAL :
0.09% 叠氮化钠
缓冲液CRYOPRESERVATIVE :
50% 甘油
靶标背景 :
VGlut1 is expressed in a subset of glutamate neurons where it transports glutamate into native synaptic vesicles from the brain. Glutamate transport by VGlut1 is saturated with a K(m) of approximately 2mM. Whereas plasma membrane glutamate transporters recognize both aspartate and glutamate as substrates, VGlut1 recognizes only glutamate.
FUNCTION :
Mediates the uptake of glutamate into synaptic vesicles at presynaptic nerve terminals of excitatory neural cells. May also mediate the transport of inorganic phosphate. {PubMed:10938000, PubMed:11001057, PubMed:11698619, PubMed:11698620, PubMed:18080752, PubMed:8202535}.
稀释INSTRUCTIONS :
Dilute in PBS or medium which is identical to that used in the assay system.
STORAGE空调 :
This product is stable for at least 1 year at -20C. Freeze in multiple aliquots to avoid repeated freeze-thaw cycles.
特异性 :
This antibody recognizes human, mouse, and rat VGlut1. It does not cross-react with VGlut2.
图像Name_1 :
qed-bioscience-anti-vglut1-transporter-antibody-56514-1.png
应用1 :
免疫细胞化学
图像Name_2 :
qed-bioscience-anti-vglut1-transporter-antibody-56514-2.png
应用2 :
免疫细胞化学
图像Name_3 :
qed-bioscience-anti-vglut1-transporter-antibody-56514-3.png
应用3 :
免疫组化
图像Name_4 :
qed-bioscience-anti-vglut1-transporter-antibody-56514-4.png
图像Caption_4 :
Western Blot analysis of Rat brain membrane lysate showing detection of VGLUT1 protein using Mouse Anti-VGLUT1 Monoclonal Antibody, Clone S28-9 (56514). Primary Antibody: Mouse Anti-VGLUT1 Monoclonal Antibody (56514) at 1:1000.
应用4 :
免疫印迹
更多信息或购买 :
公司信息
QED Bioscience
10919 Technology Place, Suite C San Diego, CA 92127
info@qedbio.com
http://www.qedbio.com800-929-2114 858-675-2405
公司总部: 美国