抗体

抗ATF4(磷酸化Ser245)抗体

43053

100微升

345美元

多克隆

domestic rabbit

未共轭

抗ATF4(磷酸化Ser245)抗体

抗ATF4(磷酸化Ser245)抗体(43053)

抗体

抗ATF4(磷酸化Ser245)兔多克隆抗体

ATF4(磷酸化Ser245)

人类

ATF4(磷酸化Ser245)

磷酸化ser245

P15336

Nucleus. Cytoplasm. Mitochondrion outer membrane. Note=Shuttles between the cytoplasm and the nucleus and heterodimerization with JUN is essential for the nuclear localization. Localization to the cytoplasm is observed under conditions of cellular stress and in disease states. Localizes at the mitochondrial outer membrane in response to genotoxic stress. Phosphorylation at Thr-52 is required for its nuclear localization and negatively regulates its mitochondrial localization. Co-localizes with the MRN complex in the IR-induced foci (IRIF).

Cyclic AMP-dependent transcription factor ATF-2

ATF2

多克隆

液体

1.0毫克/毫升

100微升

345美元

241.5

43053

免疫印迹, 免疫组化

Immunoblotting: use at dilution of 1:500-1:1,000. A band of ~45kDa is detected. Immunohistochemistry: use at dilution of 1:50- 1:100. These are recommended working dilutions. Enduser should determine optimal dilutions for their applications.

兔

纯化

经免疫亲和层析纯化

Peptide sequence that includes phosphorylation sites of serine 245 (N-R-S(p)-L-P) derived from human ATF4 and conjugated to KLH.

磷酸化特异性抗体

PBS (without Mg2 and Ca2 ), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

磷酸盐缓冲液

pH值7.4

0.02% 叠氮化钠

50% 甘油

ATF4 encodes a transcription factor that was originally identified as a widely expressed mammalian DNA-binding protein that could bind a tax-responsive enhancer element in the LTR of HTLV-1. ATF4 belongs to a family of DNA-binding proteins that includes the AP-1 family of transcription factors, cAMP-response element binding proteins (CREBs) and CREB-like proteins. These transcription factors share a leucine zipper region that is involved in protein-protein interactions located C-terminal to a stretch of basic amino acids that functions as a DNA binding domain. Diseases associated with ATF4 include leukoencephalopathy with vanishing white matter and T-cell leukemia.

Transcriptional activator which regulates the transcription of various genes, including those involved in anti-apoptosis, cell growth, and DNA damage response. Dependent on its binding partner, binds to CRE (cAMP response element) consensus sequences (5'-TGACGTCA-3') or to AP-1 (activator protein 1) consensus sequences (5'-TGACTCA-3'). In the nucleus, contributes to global transcription and the DNA damage response, in addition to specific transcriptional activities that are related to cell development, proliferation and death. In the cytoplasm, interacts with and perturbs HK1- and VDAC1-containing complexes at the mitochondrial outer membrane, thereby impairing mitochondrial membrane potential, inducing mitochondrial leakage and promoting cell death. The phosphorylated form (mediated by ATM) plays a role in the DNA damage response and is involved in the ionizing radiation (IR)-induced S phase checkpoint control and in the recruitment of the MRN complex into the IR-induced foci (IRIF). Exhibits histone acetyltransferase (HAT) activity which specifically acetylates histones H2B and H4 in vitro (PubMed:10821277). In concert with CUL3 and RBX1, promotes the degradation of KAT5 thereby attenuating its ability to acetylate and activate ATM. Can elicit oncogenic or tumor suppressor activities depending on the tissue or cell type. {PubMed:10821277, PubMed:15916964, PubMed:18397884, PubMed:22304920}.

Dilute in PBS or medium that is identical to that used in the assay system.

This antibody is stable for at least one (1) year at -20C. Can be stored at 4C for short-term use.

This antibody detects endogenous human ATF4 only when phosphorylated at serine 245.