产品简要
公司名称 :
QED Bioscience
产品类型 :
抗体
产品名称 :
抗TSG101抗体
目录 :
3352
规格 :
100微升
价格 :
461美元
克隆性 :
单克隆
宿主 :
小鼠
共轭标签 :
未共轭
克隆名称 :
4A10
反应物种 :
人类, 小鼠, 大鼠
更多信息或购买 :
图像
图像 1 :
QED Bioscience 3352 图像 1
TSG101 antibody [4A10] detects TSG101 protein by western blot analysis. A. 30 ug NIH-3T3 whole cell lysate/extract B. 30 ug JC whole cell lysate/extract C. 30 ug BCL-1 whole cell lysate/extract 10% SDS-PAGE TSG101 antibody [4A10] (3352) dilution: 1:500 The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
图像 2 :
QED Bioscience 3352 图像 2
ELISA detection of TSG101 using for capture at a concentration of 5 ug/mL and 3352 for detection at a concentration of 1.5 ug/mL.
图像 3 :
QED Bioscience 3352 图像 3
Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with TSG101 antibody [4A10] (3352) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
产品信息
目录号 :
3352
产品名称 :
抗TSG101抗体
NAME+SKU :
抗TSG101抗体(3352)
产品_TYPE :
抗体
描述 :
抗TSG101小鼠单克隆抗体
SPECIFICITY SHORT :
TSG101
物种活性 :
Human, mouse, rat, hamster, and monkey
靶标名 :
TSG101
UniProt数据库 :
Q99816
LOCATION :
Cytoplasm , Early endosome membrane , Late endosome membrane , Midbody, Midbody ring , Nucleus
蛋白名 :
Tumor susceptibility gene 101 protein
别称 :
ESCRT-I complex subunit TSG101
基因名称 :
TSG101
登录号 :
NP_006283
克隆性 :
单克隆
形式 :
液体
浓度 :
Lot Specific
规格1 :
100微升
标价规格1 :
461美元
DISTIRBUTOR价格 :
322.7
SKU号规格1 :
3352
应用 :
ELSIA, 免疫印迹, 免疫组化
应用INSTRUCTIONS :
ELISA: use at 1-5ug/ml. Immunoblotting: use at a 1:500-1:3,000 dilution. A band of 46kDa is detected. Detection of TSG101 protein in (A) NIH-3T3 cell lysate, (B) JC cell lysate, and (C) BCL-1 cell lysate with #3352 diluted 1:500. Endusers should determine optimal antibody concentrations for their applications. IHC: use at a dilution of 1:100 with antigen retrieval: citrate buffer, pH 6.0, 15 minutes. Detection of TSG101 protein in paraffin-embedded human breast carcinoma. Detection of TSG101 protein in paraffin-embedded human ovarian carcinoma.
宿主 :
小鼠
格式 :
纯化
抗体亚型 :
IgG1
克隆编号 :
4A10
免疫原 :
Recombinant protein corresponding to aa 167-374 of TSG101 protein
研究领域 :
Cancer Research
配方 :
PBS, pH值7.2
缓冲液 :
磷酸盐缓冲液
缓冲液pH值 :
pH值7.2
FUNCTION :
Component of the ESCRT-I complex, a regulator of vesicular trafficking process. Binds to ubiquitinated cargo proteins and is required for the sorting of endocytic ubiquitinated cargos into multivesicular bodies (MVBs). Mediates the association between the ESCRT-0 and ESCRT-I complex. Required for completion of cytokinesis; the function requires CEP55. May be involved in cell growth and differentiation. Acts as a negative growth regulator. Involved in the budding of many viruses through an interaction with viral proteins that contain a late-budding motif P-[ST]-A-P. This interaction is essential for viral particle budding of numerous retroviruses. Required for the exosomal release of SDCBP, CD63 and syndecan (PubMed:22660413). It may also play a role in the extracellular release of microvesicles that differ from the exosomes (PubMed:22315426). {PubMed:11916981, PubMed:17556548, PubMed:17853893, PubMed:21070952, PubMed:21757351, PubMed:22315426, PubMed:22660413}.
STORAGE空调 :
This antibody is stable for at least one (1) year at -20C.
特异性 :
This antibody recognizes human, mouse, rat, hamster, and monkey TSG101 protein.
图像Name_1 :
qed-bioscience-anti-tsg101-antibody-3352-1.jpg
应用1 :
免疫印迹
图像Name_2 :
qed-bioscience-anti-tsg101-antibody-3352-2.jpg
应用2 :
酶联免疫吸附测定
图像Name_3 :
qed-bioscience-anti-tsg101-antibody-3352-3.jpg
应用3 :
免疫印迹
图像Name_4 :
qed-bioscience-anti-tsg101-antibody-3352-4.jpg
图像Caption_4 :
TSG101 antibody [4A10] detects TSG101 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. TSG101 stained by TSG101 antibody [4A10] (3352) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
应用4 :
免疫组化
图像Name_5 :
qed-bioscience-anti-tsg101-antibody-3352-5.jpg
图像Caption_5 :
TSG101 antibody [4A10] detects TSG101 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded human ovarian cancer. TSG101 stained by TSG101 antibody [4A10] (3352) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
应用5 :
免疫组化
图像Name_6 :
qed-bioscience-anti-tsg101-antibody-3352-6.jpg
图像Caption_6 :
TSG101 antibody [4A10] (3352) detects TSG101 protein by flow cytometry analysis. Sample: THP-1 cell. Black: Unlabelled sample was used as a control. Red: TSG101 antibody [4A10] (3352) dilution: 1:25. Acquisition of 20,000 events were collected using a Dylight 488-conjugated secondary antibody for FACS analysis.
应用6 :
FC
图像Name_7 :
qed-bioscience-anti-tsg101-antibody-3352-7.jpg
图像Caption_7 :
Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with TSG101 antibody [4A10] (3352) diluted at 1:500. The HRP-conjugated anti-mouset IgG antibody was used to detect the primary antibody.
应用7 :
免疫印迹
图像Name_8 :
qed-bioscience-anti-tsg101-antibody-3352-8.jpg
图像Caption_8 :
Various tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with TSG101 antibody [4A10] (3352) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
应用8 :
免疫印迹
图像Name_9 :
qed-bioscience-anti-tsg101-antibody-3352-9.jpg
图像Caption_9 :
TSG101 antibody [4A10] detects TSG101 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded human lung cancer. TSG101 stained by TSG101 antibody [4A10] (3352) diluted at 1:50. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
应用9 :
免疫组化
图像Name_10 :
qed-bioscience-anti-tsg101-antibody-3352-10.jpg
图像Caption_10 :
Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with TSG101 antibody [4A10] (3352) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
应用10 :
免疫印迹
图像Name_11 :
qed-bioscience-anti-tsg101-antibody-3352-11.jpg
图像Caption_11 :
Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with TSG101 antibody [4A10] (3352) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
更多信息或购买 :
公司信息
QED Bioscience
10919 Technology Place, Suite C San Diego, CA 92127
info@qedbio.com
http://www.qedbio.com
800-929-2114 858-675-2405
公司总部: 美国