产品简要
公司名称 :
QED Bioscience
产品类型 :
抗体
产品名称 :
抗哺乳动物雷帕霉素靶蛋白抗体
目录 :
13036
规格 :
100微克
价格 :
503美元
克隆性 :
多克隆
宿主 :
domestic rabbit
共轭标签 :
未共轭
反应物种 :
人类, 小鼠, 大鼠
更多信息或购买 :
图像
图像 1 :
![QED Bioscience 13036 图像 1](http://www.labome.com/product/QED-Bioscience/6378981_0.jpg)
Detection of human mTOR by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-mTOR antibody 13036 (lot 13036-2) used for IP at 6 µg per reaction. mTOR was also immunoprecipitated by rabbit anti-mTOR antibody. For blotting immunoprecipitated mTOR, 13036 was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
图像 2 :
![QED Bioscience 13036 图像 2](http://www.labome.com/product/QED-Bioscience/6378981_1.jpg)
Detection of human mTOR by western blot. Samples: Whole cell lysate (50 µg) from HeLa and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-mTOR antibody 13036 (lot 13036-2) used for WB at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
产品信息
目录号 :
13036
产品名称 :
抗哺乳动物雷帕霉素靶蛋白抗体
NAME+SKU :
抗哺乳动物雷帕霉素靶蛋白抗体(13036)
产品_TYPE :
抗体
描述 :
抗哺乳动物雷帕霉素靶蛋白兔多克隆抗体
SPECIFICITY SHORT :
哺乳动物雷帕霉素靶蛋白
物种活性 :
人类, 小鼠, 大鼠
靶标名 :
哺乳动物雷帕霉素靶蛋白
UniProt数据库 :
P42345
LOCATION :
Endoplasmic reticulum membrane , Golgi apparatus membrane , Mitochondrion outer membrane , Lysosome , Cytoplasm , Nucleus, PML body , Microsome membrane , Lysosome membrane , Cytoplasmic vesicle, phagosome
蛋白名 :
丝氨酸/苏氨酸蛋白激酶哺乳动物雷帕霉素靶蛋白
别称 :
EC 2.7.11.1, FK506-binding protein 12-rapamycin complex-associated protein 1, FKBP12-rapamycin complex-associated protein, Mammalian target of rapamycin, mTOR, Mechanistic target of rapamycin, Rapamycin and FKBP12 target 1, Rapamycin target protein 1
基因名称 :
哺乳动物雷帕霉素靶蛋白
登录号 :
NP_004949
克隆性 :
多克隆
形式 :
液体
浓度 :
Lot Specific
规格1 :
100微克
标价规格1 :
503美元
DISTIRBUTOR价格 :
352.1
SKU号规格1 :
13036
应用 :
免疫印迹, 免疫沉淀
应用INSTRUCTIONS :
Western Blot: 0.1-1 ug/ml.
Note: Overnight incubation at 4°C is recommended for primary antibody. MTOR requires detergent and extended Western Blotting transfer times (e.g. in 5% acrylamide gels to PVDF, use 50V for 3 hours in CAPS buffer with 10% methanol and 0.1% SDS).
Immunoprecipitation: Use Catalog Number 13034.
宿主 :
兔
格式 :
纯化
纯化 :
经免疫亲和层析纯化
免疫原 :
Synthetic peptide representing a portion of the protein encoded in part by exons 5 and 6 (LocusLink ID 1981).
研究领域 :
酶
配方 :
PBS含0.1% 叠氮化钠
缓冲液 :
磷酸盐缓冲液
缓冲液抗MICROBIAL :
0.1% 叠氮化钠
FUNCTION :
Serine/threonine protein kinase which is a central regulator of cellular metabolism, growth and survival in response to hormones, growth factors, nutrients, energy and stress signals (PubMed:12087098, PubMed:12150925, PubMed:12150926, PubMed:12231510, PubMed:12718876, PubMed:14651849, PubMed:15268862, PubMed:15467718, PubMed:15545625, PubMed:15718470, PubMed:18497260, PubMed:18762023, PubMed:18925875, PubMed:20516213, PubMed:20537536, PubMed:21659604, PubMed:23429703, PubMed:23429704, PubMed:25799227, PubMed:26018084). MTOR directly or indirectly regulates the phosphorylation of at least 800 proteins. Functions as part of 2 structurally and functionally distinct signaling complexes mTORC1 and mTORC2 (mTOR complex 1 and 2) (PubMed:15268862, PubMed:15467718, PubMed:18925875, PubMed:18497260, PubMed:20516213, PubMed:21576368, PubMed:21659604, PubMed:23429704). Activated mTORC1 up-regulates protein synthesis by phosphorylating key regulators of mRNA translation and ribosome synthesis (PubMed:12087098, PubMed:12150925, PubMed:12150926, PubMed:12231510, PubMed:12718876, PubMed:14651849, PubMed:15268862, PubMed:15467718, PubMed:15545625, PubMed:15718470, PubMed:18497260, PubMed:18762023, PubMed:18925875, PubMed:20516213, PubMed:20537536, PubMed:21659604, PubMed:23429703, PubMed:23429704, PubMed:25799227, PubMed:26018084). This includes phosphorylation of EIF4EBP1 and release of its inhibition toward the elongation initiation factor 4E (eiF4E) (By similarity). Moreover, phosphorylates and activates RPS6KB1 and RPS6KB2 that promote protein synthesis by modulating the activity of their downstream targets including ribosomal protein S6, eukaryotic translation initiation factor EIF4B, and the inhibitor of translation initiation PDCD4 (PubMed:12150925, PubMed:12087098, PubMed:18925875). This also includes mTORC1 signaling cascade controlling the MiT/TFE factors TFEB and TFE3: in the presence of nutrients, mediates phosphorylation of TFEB and TFE3, promoting their cytosolic retention and inactivation (PubMed:22576015, PubMed:22343943, PubMed:22692423). Upon starvation or lysosomal stress, inhibition of mTORC1 induces dephosphorylation and nuclear translocation of TFEB and TFE3, promoting their transcription factor activity (PubMed:22576015, PubMed:22343943, PubMed:22692423). Stimulates the pyrimidine biosynthesis pathway, both by acute regulation through RPS6KB1-mediated phosphorylation of the biosynthetic enzyme CAD, and delayed regulation, through transcriptional enhancement of the pentose phosphate pathway which produces 5-phosphoribosyl-1-pyrophosphate (PRPP), an allosteric activator of CAD at a later step in synthesis, this function is dependent on the mTORC1 complex (PubMed:23429704, PubMed:23429703). Regulates ribosome synthesis by activating RNA polymerase III-dependent transcription through phosphorylation and inhibition of MAF1 an RNA polymerase III-repressor (PubMed:20516213). In parallel to protein synthesis, also regulates lipid synthesis through SREBF1/SREBP1 and LPIN1 (By similarity). To maintain energy homeostasis mTORC1 may also regulate mitochondrial biogenesis through regulation of PPARGC1A (By similarity). mTORC1 also negatively regulates autophagy through phosphorylation of ULK1 (By similarity). Under nutrient sufficiency, phosphorylates ULK1 at 'Ser-758', disrupting the interaction with AMPK and preventing activation of ULK1 (By similarity). Also prevents autophagy through phosphorylation of the autophagy inhibitor DAP (PubMed:20537536). Also prevents autophagy by phosphorylating RUBCNL/Pacer under nutrient-rich conditions (PubMed:30704899). Prevents autophagy by mediating phosphorylation of AMBRA1, thereby inhibiting AMBRA1 ability to mediate ubiquitination of ULK1 and interaction between AMBRA1 and PPP2CA (PubMed:23524951, PubMed:25438055). mTORC1 exerts a feedback control on upstream growth factor signaling that includes phosphorylation and activation of GRB10 a INSR-dependent signaling suppressor (PubMed:21659604). Among other potential targets mTORC1 may phosphorylate CLIP1 and regulate microtubules (PubMed:12231510). As part of the mTORC2 complex MTOR may regulate other cellular processes including survival and organization of the cytoskeleton (PubMed:15268862, PubMed:15467718). Plays a critical role in the phosphorylation at 'Ser-473' of AKT1, a pro-survival effector of phosphoinositide 3-kinase, facilitating its activation by PDK1 (PubMed:15718470). mTORC2 may regulate the actin cytoskeleton, through phosphorylation of PRKCA, PXN and activation of the Rho-type guanine nucleotide exchange factors RHOA and RAC1A or RAC1B (PubMed:15268862). mTORC2 also regulates the phosphorylation of SGK1 at 'Ser-422' (PubMed:18925875). Regulates osteoclastogenesis by adjusting the expression of CEBPB isoforms (By similarity). Plays an important regulatory role in the circadian clock function; regulates period length and rhythm amplitude of the suprachiasmatic nucleus (SCN) and liver clocks (By similarity). Phosphorylates SQSTM1, promoting interaction between SQSTM1 and KEAP1 and subsequent inactivation of the BCR(KEAP1) complex (By similarity). {UniProtKB:P42346, UniProtKB:Q9JLN9, PubMed:12087098, PubMed:12150925, PubMed:12150926, PubMed:12231510, PubMed:12718876, PubMed:14651849, PubMed:15268862, PubMed:15467718, PubMed:15545625, PubMed:15718470, PubMed:18497260, PubMed:18762023, PubMed:18925875, PubMed:20516213, PubMed:20537536, PubMed:21576368, PubMed:21659604, PubMed:22343943, PubMed:22576015, PubMed:22692423, PubMed:23429703, PubMed:23429704, PubMed:23524951, PubMed:25438055, PubMed:25799227, PubMed:26018084, PubMed:30704899}.
STORAGE空调 :
Store at 2 – 8C. Antibody is stable at 2 – 8C for 1 year.
特异性 :
This antibody reacts with human, mouse and rat. Antibody is affinity purified.
图像Name_1 :
qed-bioscience-mtor-antibody-13036-1.jpg
应用1 :
免疫印迹
图像Name_2 :
qed-bioscience-mtor-antibody-13036-2.jpg
应用2 :
免疫印迹
更多信息或购买 :
公司信息
![logo](http://aws.labome.com/img/273.png)
QED Bioscience
10919 Technology Place, Suite C San Diego, CA 92127
info@qedbio.com
http://www.qedbio.com800-929-2114 858-675-2405
公司总部: 美国
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