产品简要
公司名称 :
QED Bioscience
产品类型 :
抗体
产品名称 :
抗DNMT1抗体
目录 :
12595
规格 :
100微克
价格 :
347美元
克隆性 :
单克隆
宿主 :
小鼠
共轭标签 :
未共轭
克隆名称 :
4G11-C7
反应物种 :
人类, 小鼠, 斑马鱼
更多信息或购买 :
产品信息
目录号 :
12595
产品名称 :
抗DNMT1抗体
NAME+SKU :
抗DNMT1抗体(12595)
产品_TYPE :
抗体
描述 :
抗DNMT1小鼠单克隆抗体
SPECIFICITY SHORT :
DNMT1
物种活性 :
人类, 小鼠, 斑马鱼
靶标名 :
DNMT1
UniProt数据库 :
P26358
LOCATION :
Nucleus
蛋白名 :
脱氧核糖核酸
别称 :
胞嘧啶5 1
基因名称 :
DNMT1
登录号 :
NP_001370
克隆性 :
单克隆
形式 :
液体
浓度 :
Lot Specific
规格1 :
100微克
标价规格1 :
347美元
DISTIRBUTOR价格 :
242.9
SKU号规格1 :
12595
应用 :
免疫印迹, 酶联免疫吸附测定
应用INSTRUCTIONS :
ELISA Immunoblotting: use at 2ug/ml. A band of ~190kDa is detected.
These are recommended concentrations; enduser should determine optimal concentrations for their applications.
Positive controls: ES and H1299 cell lysates.
宿主 :
小鼠
格式 :
纯化
纯化 :
经免疫亲和层析纯化
抗体亚型 :
IgG2b
克隆编号 :
4G11-C7
免疫原 :
合成多肽对应于氨基酸637-650人的DNMT1(登录号。 NP_001370)
研究领域 :
脱氧核糖核酸
配方 :
PBS, pH 7.4, 0.09% sodium azide.
Purified by Protein G affinity chromatography.
缓冲液 :
磷酸盐缓冲液
缓冲液pH值 :
pH值7.4
缓冲液抗MICROBIAL :
0.09% 叠氮化钠
靶标背景 :
Methylation of DNA at cytosine residues is important in regulation of gene expression and genomic imprinting. Hyper- methylation of CpG islands in tumor suppressor genes or hypomethylation of bulk genomic DNA may be linked to development of cancer. Thus far three families of mammalian DNA methyl- transferase genes have been identified: DNMT1, DNMT2, and DNMT3. DNMT1 is constitutively expressed in proliferating cells, and inactivation of this gene causes global demethylation of genomic DNA and embryonic lethality. DNMT1 co-purifies with the retinoblastoma (Rb) tumor suppressor gene product E2F1 and with HDAC1. DNMT1 cooperates with Rb to repress transcription from promoters containing E2F-binding sites which suggests a link between DNA methylation, histone deacetylase and sequence- specific DNA binding activity as well as a growth-regulatory pathway that is disrupted in cancer cells.
FUNCTION :
Methylates CpG residues. Preferentially methylates hemimethylated DNA. Associates with DNA replication sites in S phase maintaining the methylation pattern in the newly synthesized strand, that is essential for epigenetic inheritance. Associates with chromatin during G2 and M phases to maintain DNA methylation independently of replication. It is responsible for maintaining methylation patterns established in development. DNA methylation is coordinated with methylation of histones. Mediates transcriptional repression by direct binding to HDAC2. In association with DNMT3B and via the recruitment of CTCFL/BORIS, involved in activation of BAG1 gene expression by modulating dimethylation of promoter histone H3 at H3K4 and H3K9. Probably forms a corepressor complex required for activated KRAS-mediated promoter hypermethylation and transcriptional silencing of tumor suppressor genes (TSGs) or other tumor-related genes in colorectal cancer (CRC) cells (PubMed:24623306). Also required to maintain a transcriptionally repressive state of genes in undifferentiated embryonic stem cells (ESCs) (PubMed:24623306). Associates at promoter regions of tumor suppressor genes (TSGs) leading to their gene silencing (PubMed:24623306). Promotes tumor growth (PubMed:24623306). {PubMed:16357870, PubMed:18413740, PubMed:18754681, PubMed:24623306}.
稀释INSTRUCTIONS :
Dilute in PBS or medium which is identical to that used in the assay system.
STORAGE空调 :
This antibody is stable for at least one (1) year at -20C. Can be stored at 4C for short-term use.
特异性 :
这抗体识别人类, 小鼠, 和斑马鱼DNMT1
更多信息或购买 :
公司信息
QED Bioscience
10919 Technology Place, Suite C San Diego, CA 92127
info@qedbio.com
http://www.qedbio.com800-929-2114 858-675-2405
公司总部: 美国