产品简要
公司名称 :
QED Bioscience
产品类型 :
抗体
产品名称 :
Anti-DMPO Nitrone Adduct Antibody
目录 :
12531
规格 :
100微克
价格 :
448美元
克隆性 :
单克隆
宿主 :
小鼠
共轭标签 :
未共轭
克隆名称 :
N1664A
更多信息或购买 :
QED Bioscience 产品网页
图像
图像 1 :
QED Bioscience 12531 图像 1
Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-DMPO Monoclonal Antibody, Clone N1664A (12531). Tissue: macrophage cell line (Raw 264.7). Species: Mouse. Primary Antibody: Mouse Anti-DMPO Monoclonal Antibody (12531) at 1:100. Secondary Antibody: Alexa Fluor 488 Goat Anti-Mouse (green) at 1:1000. Counterstain: DAPI (blue) nuclear stain.
图像 2 :
QED Bioscience 12531 图像 2
Western Blot analysis of Human HL 60 clone 15 eosinophils lysates showing detection of DMPO protein using Mouse Anti-DMPO Monoclonal Antibody, Clone N1664A (12531). Primary Antibody: Mouse Anti-DMPO Monoclonal Antibody (12531) at 1:200.
产品信息
目录号 :
12531
产品名称 :
Anti-DMPO Nitrone Adduct Antibody
NAME+SKU :
Anti-DMPO Nitrone Adduct Antibody (12531)
产品_TYPE :
抗体
描述 :
Anti-DMPO Nitrone Adduct Mouse Monoclonal Antibody
SPECIFICITY SHORT :
DMPO Nitrone Adduct
物种活性 :
都是
靶标名 :
DMPO Nitrone Adduct
克隆性 :
单克隆
形式 :
液体
浓度 :
Lot Specific
规格1 :
100微克
标价规格1 :
448美元
DISTIRBUTOR价格 :
313.6
SKU号规格1 :
12531
应用 :
免疫印迹, 酶联免疫吸附测定, 免疫细胞化学
应用INSTRUCTIONS :
Immunoblotting: use at 1-10ug/ml. Immunofluorescence: use at10ug/ml. These are recommended concentrations; enduser should determine optimal concentrations for their applications. ELISA: use at 1-10ug/ml Positive control: DMPO nitrone adducts of metmyoglobin.
宿主 :
小鼠
格式 :
纯化
纯化 :
经免疫亲和层析纯化
抗体亚型 :
IgG1
克隆编号 :
N1664A
免疫原 :
5,5-dimethyl-2-(8-octanoic acid)-1-pyrrolone-N-oxide conjugated to ovalbumin.
研究领域 :
脱氧核糖核酸
配方 :
PBS, pH值7.4, 50% 甘油, 0.09% 叠氮化钠
缓冲液 :
磷酸盐缓冲液
缓冲液pH值 :
pH值7.4
缓冲液抗MICROBIAL :
0.09% 叠氮化钠
缓冲液CRYOPRESERVATIVE :
50% 甘油
靶标背景 :
Free radicals are highly reactive and generally short-lived species. Hence, most studies of free radicals have been restricted to analysis of end products formed following their interaction with cellular biomolecules. One technology that has permitted direct study of free radicals is electron spin resonance (ESR). For very short lived radicals, spin traps have been employed to generate longer lived radicals that are more amenable to ESR analysis. The most widely used of these spin traps is 5,5-dimethyl-1-pyrroline-N-oxide (DMPO). The reaction of DMPO with a protein radical gives rise to a nitroxide radical which can be disproportionate to hydrozylamine and nitrone adducts. Of these species, only the nitrone adduct can be considered stable.
稀释INSTRUCTIONS :
Dilute in PBS or medium that is identical to that used in the assay system.
STORAGE空调 :
This antibody is stable for 1 month at 4°C or one year at -20°C. Avoid multiple freeze-thaw cycles.
特异性 :
This antibody recognizes DMPO, DMPO-octanoic acid, DMPO-protein adducts, and DMPO-DNA adducts. It does not cross-react with non-adducted proteins or DNA. Species independent.
图像Name_1 :
qed-bioscience-anti-dmpo-nitrone-adduct-antibody-12531-1.png
应用1 :
免疫细胞化学
图像Name_2 :
qed-bioscience-anti-dmpo-nitrone-adduct-antibody-12531-2.png
应用2 :
免疫印迹
更多信息或购买 :
QED Bioscience 产品网页
公司信息
QED Bioscience
10919 Technology Place, Suite C San Diego, CA 92127
info@qedbio.com
http://www.qedbio.com
800-929-2114 858-675-2405
公司总部: 美国