产品简要
公司名称 :
QED Bioscience
产品类型 :
抗体
产品名称 :
抗HSF1抗体
目录 :
12518
规格 :
100微克
价格 :
371美元
克隆性 :
单克隆
宿主 :
大鼠
共轭标签 :
未共轭
克隆名称 :
10H8
反应物种 :
人类, 小鼠, 大鼠, 牛
更多信息或购买 :
图像
图像 1 :
![QED Bioscience 12518 图像 1](http://www.labome.com/product/QED-Bioscience/6378953_0.png)
Immunocytochemistry/Immunofluorescence analysis using Rat Anti-HSF1 Monoclonal Antibody, Clone 10H8 (12518). Tissue: Heat Shocked cervical cancer cells (HeLa). Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rat Anti-HSF1 Monoclonal Antibody (12518) at 1:100 for 12 hours at 4°C. Secondary Antibody: FITC Goat Anti-Rat (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Diffuse nuclear and cytoplasmic staining. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-HSF1 Antibody. (C) Composite. Heat Shocked at 42°C for 1h.
图像 2 :
![QED Bioscience 12518 图像 2](http://www.labome.com/product/QED-Bioscience/6378953_1.png)
Immunocytochemistry/Immunofluorescence analysis using Rat Anti-HSF1 Monoclonal Antibody, Clone 10H8 (12518). Tissue: Heat Shocked cervical cancer cells (HeLa). Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rat Anti-HSF1 Monoclonal Antibody (12518) at 1:100 for 12 hours at 4°C. Secondary Antibody: APC Goat Anti-Rat (red) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Diffuse nuclear and cytoplasmic staining. Magnification: 20x. (A) DAPI (blue) nuclear stain. (B) Anti-HSF1 Antibody. (C) Composite. Heat Shocked at 42°C for 1h.
图像 3 :
![QED Bioscience 12518 图像 3](http://www.labome.com/product/QED-Bioscience/6378953_2.png)
Immunocytochemistry/Immunofluorescence analysis using Rat Anti-HSF1 Monoclonal Antibody, Clone 10H8 (12518). Tissue: Heat Shocked mitotic HeLa cells. Species: Human. Primary Antibody: Rat Anti-HSF1 Monoclonal Antibody (12518) at 1:1000. HSF1 stained green. Courtesy of: Morimoto Lab, Northwestern University, USA.
产品信息
目录号 :
12518
产品名称 :
抗HSF1抗体
NAME+SKU :
抗HSF1抗体(12518)
产品_TYPE :
抗体
描述 :
抗HSF1大鼠单克隆抗体
SPECIFICITY SHORT :
HSF1
物种活性 :
Human, mouse, rat, raabbit, bovine, guinea pig, hamster, monkey
靶标名 :
HSF1
UniProt数据库 :
Q00613
LOCATION :
Nucleus , Cytoplasm , Nucleus, nucleoplasm , Cytoplasm, perinuclear region , Cytoplasm, cytoskeleton, spindle pole , Cytoplasm, cytoskeleton, microtubule organizing center, centrosome , Chromosome, centromere, kinetochore
蛋白名 :
Heat shock factor protein 1
别称 :
HSF 1, 加热休克转录因子1, HSTF 1
基因名称 :
HSF1
登录号 :
NP_005517
克隆性 :
单克隆
形式 :
液体
浓度 :
Lot Specific
规格1 :
100微克
标价规格1 :
371美元
DISTIRBUTOR价格 :
259.7
SKU号规格1 :
12518
应用 :
免疫印迹, 免疫沉淀, 免疫细胞化学
应用INSTRUCTIONS :
Immunoblotting: use at 1-2ug/ml. A band of ~85 and/or ~95 kDa is detected.
Immunocytochemistry: use at 10ug/ml.
Immunoprecipitation: use at 12.5ug/ml
These are recommended concentrations. User should determine optimal concentrations for their application.
Positive control: HeLa cell lysate
宿主 :
大鼠
格式 :
纯化
纯化 :
经免疫亲和层析纯化
抗体亚型 :
IgG1
克隆编号 :
10H8
免疫原 :
重组小鼠HSF-1
研究领域 :
加热休克&压力蛋白
配方 :
PBS, pH值7.4
缓冲液 :
磷酸盐缓冲液
缓冲液pH值 :
pH值7.4
靶标背景 :
Heat shock factor 1 (HSF1) is a heat shock transcription factor that activates the transcription of genes encoding products required for protein folding, processing, targeting, degradation, and function. Up- regulation of expression of heat shock proteins in response to stress occurs at the level of transcription through a heat shock element and a HSF transcription factor. Amino acid sequences for most HSFs are highly conserved. A DNA binding domain is at the N-terminus, hydrophobic repeats (essential to the formation of active trimers) are adjacent to this binding domain, and another short hydrophobic repeat (necessary for suppression of trimerization) occurs toward the C-terminus. In higher eukaryotes, HSF1 is distributed diffusely in the cell cytoplasm and nucleus in unstressed cells. On exposure to heat shock or other stresses, HSF1 localizes to discrete nuclear granules; on recovery from stress, it returns to a diffuse nuclear- cytoplasmic distribution.
FUNCTION :
Functions as a stress-inducible and DNA-binding transcription factor that plays a central role in the transcriptional activation of the heat shock response (HSR), leading to the expression of a large class of molecular chaperones heat shock proteins (HSPs) that protect cells from cellular insults' damage (PubMed:1871105, PubMed:11447121, PubMed:1986252, PubMed:7760831, PubMed:7623826, PubMed:8946918, PubMed:8940068, PubMed:9341107, PubMed:9121459, PubMed:9727490, PubMed:9499401, PubMed:9535852, PubMed:12659875, PubMed:12917326, PubMed:15016915, PubMed:25963659, PubMed:26754925, PubMed:18451878). In unstressed cells, is present in a HSP90-containing multichaperone complex that maintains it in a non-DNA-binding inactivated monomeric form (PubMed:9727490, PubMed:11583998, PubMed:16278218). Upon exposure to heat and other stress stimuli, undergoes homotrimerization and activates HSP gene transcription through binding to site-specific heat shock elements (HSEs) present in the promoter regions of HSP genes (PubMed:1871105, PubMed:1986252, PubMed:8455624, PubMed:7935471, PubMed:7623826, PubMed:8940068, PubMed:9727490, PubMed:9499401, PubMed:10359787, PubMed:11583998, PubMed:12659875, PubMed:16278218, PubMed:25963659, PubMed:26754925). Upon heat shock stress, forms a chromatin-associated complex with TTC5/STRAP and p300/EP300 to stimulate HSR transcription, therefore increasing cell survival (PubMed:18451878). Activation is reversible, and during the attenuation and rPubMed:10359787, PubMed:11447121, PubMed:11583998, PubMed:12659875, PubMed:12917326, PubMed:14707147, PubMed:15016915, PubMed:16278218, PubMed:17897941, PubMed:18451878, PubMed:1871105, PubMed:18794143, PubMed:1986252, PubMed:25963659, PubMed:26359349, PubMed:26727489, PubMed:26754925, PubMed:7623826, PubMed:7760831, PubMed:7935471, PubMed:8455624, PubMed:8940068, PubMed:8946918, PubMed:9121459, PubMed:9341107, PubMed:9499401, PubMed:9535852, PubMed:9727490}.; (Microbial infection) Plays a role in latent human immunodeficiency virus (HIV-1) transcriptional reactivation. Binds to the HIV-1 long terminal repeat promoter (LTR) to reactivate viral transcription by recruiting cellular transcriptional elongation factors, such as CDK9, CCNT1 and EP300. {PubMed:27189267}.
稀释INSTRUCTIONS :
Dilute in PBS or medium which is identical to that used in the assay system.
STORAGE空调 :
This product is stable for at least 1 year at -20C. Freeze in multiple aliquots to avoid repeated freeze-thaw cycles.
特异性 :
This antibody recognizes an ~85 kDa protein (inactive form) in unstressed cell lysates and an ~95 kDa protein (active form) in heat-shocked cell lysates of human, mouse, rat, rabbit, bovine, guinea pig, hamster, and monkey.
图像Name_1 :
qed-bioscience-anti-hsf1-antibody-12518-1.png
应用1 :
免疫细胞化学
图像Name_2 :
qed-bioscience-anti-hsf1-antibody-12518-2.png
应用2 :
免疫细胞化学
图像Name_3 :
qed-bioscience-anti-hsf1-antibody-12518-3.png
应用3 :
免疫细胞化学
图像Name_4 :
qed-bioscience-anti-hsf1-antibody-12518-4.png
图像Caption_4 :
Immunohistochemistry analysis using Rat Anti-HSF1 Monoclonal Antibody, Clone 10H8 (12518). Tissue: Breast carcinoma. Species: Human. Fixation: 10% Formalin Solution for 20 hours at RT. Primary Antibody: Rat Anti-HSF1 Monoclonal Antibody (12518) at 1:1000 for 40 min. Secondary Antibody: Dako labeled Polymer HRP Anti-rat IgG, DAB Chromogen (brown) (Dako Envision+ System) for 30 min at RT. Counterstain: Mayer's Hematoxylin (purple/blue) nuclear stain for 1 minute at RT. Localization: Nuclear. Magnification: 100X. Courtesy of: Dr. Sandro Santagata, Harvard Medical School.
应用4 :
免疫组化
图像Name_5 :
qed-bioscience-anti-hsf1-antibody-12518-5.png
图像Caption_5 :
Western Blot analysis of Human Breast adenocarcinoma cell line (MCF7) showing detection of ~65 kDa HSF1 protein using Rat Anti-HSF1 Monoclonal Antibody, Clone 10H8 (12518). Lane 1: MW ladder. Lane 2: HSF1 null lysate prepared from mouse embryonic fibroblasts. Lane 3: MCF7 lysate (5 µg). Lane 4: MCF7 lysate (10 µg). Lane 5: MCF7 lysate (20 µg). Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Rat Anti-HSF1 Monoclonal Antibody (12518) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rat IgG: HRP for 1 hour at RT. Predicted/Observed Size: ~65 kDa. Courtesy of: Dr. Sandro Santagata, Harvard Medical School.
应用5 :
免疫印迹
更多信息或购买 :
公司信息
![logo](http://aws.labome.com/img/273.png)
QED Bioscience
10919 Technology Place, Suite C San Diego, CA 92127
info@qedbio.com
http://www.qedbio.com800-929-2114 858-675-2405
公司总部: 美国
微博分享
关注我们的微博
- 来邦网
- 英文来邦
问题和意见