抗体

抗HSF1抗体

12518

100微克

371美元

单克隆

大鼠

未共轭

10H8

人类, 小鼠, 大鼠, 牛

抗HSF1抗体

抗HSF1抗体(12518)

抗体

抗HSF1大鼠单克隆抗体

HSF1

Human, mouse, rat, raabbit, bovine, guinea pig, hamster, monkey

HSF1

Q00613

Nucleus , Cytoplasm , Nucleus, nucleoplasm , Cytoplasm, perinuclear region , Cytoplasm, cytoskeleton, spindle pole , Cytoplasm, cytoskeleton, microtubule organizing center, centrosome , Chromosome, centromere, kinetochore

Heat shock factor protein 1

HSF 1, 加热休克转录因子1, HSTF 1

HSF1

NP_005517

单克隆

液体

Lot Specific

100微克

371美元

259.7

12518

免疫印迹, 免疫沉淀, 免疫细胞化学

Immunoblotting: use at 1-2ug/ml. A band of ~85 and/or ~95 kDa is detected. Immunocytochemistry: use at 10ug/ml. Immunoprecipitation: use at 12.5ug/ml These are recommended concentrations. User should determine optimal concentrations for their application. Positive control: HeLa cell lysate

大鼠

纯化

经免疫亲和层析纯化

IgG1

10H8

重组小鼠HSF-1

加热休克&压力蛋白

PBS, pH值7.4

磷酸盐缓冲液

pH值7.4

Heat shock factor 1 (HSF1) is a heat shock transcription factor that activates the transcription of genes encoding products required for protein folding, processing, targeting, degradation, and function. Up- regulation of expression of heat shock proteins in response to stress occurs at the level of transcription through a heat shock element and a HSF transcription factor. Amino acid sequences for most HSFs are highly conserved. A DNA binding domain is at the N-terminus, hydrophobic repeats (essential to the formation of active trimers) are adjacent to this binding domain, and another short hydrophobic repeat (necessary for suppression of trimerization) occurs toward the C-terminus. In higher eukaryotes, HSF1 is distributed diffusely in the cell cytoplasm and nucleus in unstressed cells. On exposure to heat shock or other stresses, HSF1 localizes to discrete nuclear granules; on recovery from stress, it returns to a diffuse nuclear- cytoplasmic distribution.

Functions as a stress-inducible and DNA-binding transcription factor that plays a central role in the transcriptional activation of the heat shock response (HSR), leading to the expression of a large class of molecular chaperones heat shock proteins (HSPs) that protect cells from cellular insults' damage (PubMed:1871105, PubMed:11447121, PubMed:1986252, PubMed:7760831, PubMed:7623826, PubMed:8946918, PubMed:8940068, PubMed:9341107, PubMed:9121459, PubMed:9727490, PubMed:9499401, PubMed:9535852, PubMed:12659875, PubMed:12917326, PubMed:15016915, PubMed:25963659, PubMed:26754925, PubMed:18451878). In unstressed cells, is present in a HSP90-containing multichaperone complex that maintains it in a non-DNA-binding inactivated monomeric form (PubMed:9727490, PubMed:11583998, PubMed:16278218). Upon exposure to heat and other stress stimuli, undergoes homotrimerization and activates HSP gene transcription through binding to site-specific heat shock elements (HSEs) present in the promoter regions of HSP genes (PubMed:1871105, PubMed:1986252, PubMed:8455624, PubMed:7935471, PubMed:7623826, PubMed:8940068, PubMed:9727490, PubMed:9499401, PubMed:10359787, PubMed:11583998, PubMed:12659875, PubMed:16278218, PubMed:25963659, PubMed:26754925). Upon heat shock stress, forms a chromatin-associated complex with TTC5/STRAP and p300/EP300 to stimulate HSR transcription, therefore increasing cell survival (PubMed:18451878). Activation is reversible, and during the attenuation and rPubMed:10359787, PubMed:11447121, PubMed:11583998, PubMed:12659875, PubMed:12917326, PubMed:14707147, PubMed:15016915, PubMed:16278218, PubMed:17897941, PubMed:18451878, PubMed:1871105, PubMed:18794143, PubMed:1986252, PubMed:25963659, PubMed:26359349, PubMed:26727489, PubMed:26754925, PubMed:7623826, PubMed:7760831, PubMed:7935471, PubMed:8455624, PubMed:8940068, PubMed:8946918, PubMed:9121459, PubMed:9341107, PubMed:9499401, PubMed:9535852, PubMed:9727490}.; (Microbial infection) Plays a role in latent human immunodeficiency virus (HIV-1) transcriptional reactivation. Binds to the HIV-1 long terminal repeat promoter (LTR) to reactivate viral transcription by recruiting cellular transcriptional elongation factors, such as CDK9, CCNT1 and EP300. {PubMed:27189267}.

Dilute in PBS or medium which is identical to that used in the assay system.

This product is stable for at least 1 year at -20C. Freeze in multiple aliquots to avoid repeated freeze-thaw cycles.

This antibody recognizes an ~85 kDa protein (inactive form) in unstressed cell lysates and an ~95 kDa protein (active form) in heat-shocked cell lysates of human, mouse, rat, rabbit, bovine, guinea pig, hamster, and monkey.

qed-bioscience-anti-hsf1-antibody-12518-1.png

免疫细胞化学

qed-bioscience-anti-hsf1-antibody-12518-2.png

免疫细胞化学

qed-bioscience-anti-hsf1-antibody-12518-3.png

免疫细胞化学

qed-bioscience-anti-hsf1-antibody-12518-4.png

Immunohistochemistry analysis using Rat Anti-HSF1 Monoclonal Antibody, Clone 10H8 (12518). Tissue: Breast carcinoma. Species: Human. Fixation: 10% Formalin Solution for 20 hours at RT. Primary Antibody: Rat Anti-HSF1 Monoclonal Antibody (12518) at 1:1000 for 40 min. Secondary Antibody: Dako labeled Polymer HRP Anti-rat IgG, DAB Chromogen (brown) (Dako Envision+ System) for 30 min at RT. Counterstain: Mayer's Hematoxylin (purple/blue) nuclear stain for 1 minute at RT. Localization: Nuclear. Magnification: 100X. Courtesy of: Dr. Sandro Santagata, Harvard Medical School.

免疫组化

qed-bioscience-anti-hsf1-antibody-12518-5.png

Western Blot analysis of Human Breast adenocarcinoma cell line (MCF7) showing detection of ~65 kDa HSF1 protein using Rat Anti-HSF1 Monoclonal Antibody, Clone 10H8 (12518). Lane 1: MW ladder. Lane 2: HSF1 null lysate prepared from mouse embryonic fibroblasts. Lane 3: MCF7 lysate (5 µg). Lane 4: MCF7 lysate (10 µg). Lane 5: MCF7 lysate (20 µg). Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Rat Anti-HSF1 Monoclonal Antibody (12518) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rat IgG: HRP for 1 hour at RT. Predicted/Observed Size: ~65 kDa. Courtesy of: Dr. Sandro Santagata, Harvard Medical School.

免疫印迹