抗体

抗GRP78抗体

11135

100微克

347美元

单克隆

小鼠

未共轭

6H4-2G7

人类, 小鼠, 大鼠, 牛

抗GRP78抗体

抗GRP78抗体(11135)

抗体

抗Grp78小鼠单克隆抗体

Grp78

Human, mouse, rat, bovine, hamster, rabbit, monkey, Xenopus, fungal (Cryphonectria parasitica)

GRP78

P20029

Endoplasmic reticulum lumen , Melanosome , Cytoplasm , Cell surface. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV (By similarity). Localizes to the cell surface in epithelial cells; high levels of free iron promotes cell surface localization (By similarity).

Endoplasmic reticulum chaperone BiP

EC 3.6.4.10, 78 kDa glucose-regulated protein, GRP-78, Binding-immunoglobulin protein, BiP, Heat shock protein 70 family protein 5, HSP70 family protein 5, Heat shock protein family A member 5, Immunoglobulin heavy chain-binding protein

Hspa5

NP_001156906.1

单克隆

液体

Lot Specific

100微克

347美元

242.9

11135

免疫印迹, 酶联免疫吸附测定

ELISA Immunoblotting: use at 0.5-1.0ug/ml. A band of ~78kDa is detected. Positive control: HeLa cell lysate.

小鼠

纯化

经免疫亲和层析纯化

IgG1

6H4-2G7

多聚组氨酸标签人类GRP78(登录号。 NP_001156906.1)

加热休克&压力蛋白

PBS, pH 7.4, 0.09% sodium azide. Purified by Protein G affinity chromatography.

磷酸盐缓冲液

pH值7.4

0.09% 叠氮化钠

GRP78 is a ubiquitously expressed, 78kDa glucose-regulated protein commonly referred to as an immunoglobulin chain binding protein (BiP). Categorized as stress response proteins, BiPs play an important role in folding and assembly of nascent proteins and in scavenging of misfolded proteins in the endoplasmic reticulum lumen. Translation of BiP is directed by an internal ribosomal entry site (IRES) in the 5- nontranslated region of BiP mRNA. BiP IRES activity increases when cells are heat-stressed. GRP78 is also important in maintenance of cell homeostasis and the prevention of apoptosis. Levels of GRP78 protein are reduced in brains of Alzheimer’s disease patients.

Endoplasmic reticulum chaperone that plays a key role in protein folding and quality control in the endoplasmic reticulum lumen (PubMed:12411443, PubMed:12475965). Involved in the correct folding of proteins and degradation of misfolded proteins via its interaction with DNAJC10/ERdj5, probably to facilitate the release of DNAJC10/ERdj5 from its substrate (PubMed:12411443). Acts as a key repressor of the ERN1/IRE1-mediated unfolded protein response (UPR) (By similarity). In the unstressed endoplasmic reticulum, recruited by DNAJB9/ERdj4 to the luminal region of ERN1/IRE1, leading to disrupt the dimerization of ERN1/IRE1, thereby inactivating ERN1/IRE1 (By similarity). Accumulation of misfolded protein in the endoplasmic reticulum causes release of HSPA5/BiP from ERN1/IRE1, allowing homodimerization and subsequent activation of ERN1/IRE1 (By similarity). Plays an auxiliary role in post-translational transport of small presecretory proteins across endoplasmic reticulum (ER). May function as an allosteric modulator for SEC61 channel-forming translocon complex, likely cooperating with SEC62 to enable the productive insertion of these precursors into SEC61 channel. Appears to specifically regulate translocation of precursors having inhibitory residues in their mature region that weaken channel gating. May also play a role in apoptosis and cell proliferation (By similarity). {UniProtKB:G3I8R9, UniProtKB:P11021, PubMed:12411443, PubMed:12475965}.

Dilute in PBS or medium which is identical to that used in the assay system.

This antibody is stable for at least one (1) year at -20C. Avoid repeated freeze-thaw cycles.

这抗体识别人类GRP78

qed-bioscience-anti-grp78-antibody-11135-1.jpg

免疫印迹