产品简要
公司名称 :
QED Bioscience
产品类型 :
抗体
产品名称 :
抗Hsp70抗体
目录 :
11117
规格 :
100微克
价格 :
418美元
克隆性 :
单克隆
宿主 :
小鼠
共轭标签 :
未共轭
克隆名称 :
3A3
反应物种 :
人类, 小鼠, 大鼠
更多信息或购买 :
QED Bioscience 产品网页
图像
图像 1 :
QED Bioscience 11117 图像 1
Western Blot analysis of Artemia franciscanna (brine shrimp) cell lysates showing detection of Hsp70 protein using Mouse Anti-Hsp70 Monoclonal Antibody, Clone 3A3 (11117). Primary Antibody: Mouse Anti-Hsp70 Monoclonal Antibody (11117) at 1:1000. Courtesy of: Alison King.
图像 2 :
QED Bioscience 11117 图像 2
Western Blot analysis of Rat cell lysates showing detection of Hsp70 protein using Mouse Anti-Hsp70 Monoclonal Antibody, Clone 3A3 (11117). Load: 15 µg. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Hsp70 Monoclonal Antibody (11117) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.
图像 3 :
QED Bioscience 11117 图像 3
Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-HSP70 Monoclonal Antibody, Clone 3A3 (11117). Tissue: Cervical cancer cell line (HeLa). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-HSP70 Monoclonal Antibody (11117) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: DAPI (blue) nuclear stain at 1:5000 for 5 min RT. Localization: Cytoplasm. Magnification: 40X.
产品信息
目录号 :
11117
产品名称 :
抗Hsp70抗体
NAME+SKU :
抗Hsp70抗体(11117)
产品_TYPE :
抗体
描述 :
抗Hsp70克隆3A3小鼠单克隆抗体
SPECIFICITY SHORT :
Hsp70克隆3A3
物种活性 :
Human, mouse, rat, fish, avian, amphibian, Drosophila, yeast
靶标名 :
Hsp70
UniProt数据库 :
P08107
登录号 :
NP_005336.3
克隆性 :
单克隆
形式 :
液体
浓度 :
Lot Specific
规格1 :
100微克
标价规格1 :
418美元
DISTIRBUTOR价格 :
292.6
SKU号规格1 :
11117
应用 :
免疫印迹, 免疫组化, 免疫沉淀
应用INSTRUCTIONS :
Immunoblotting: use at 0.2ug/ml (ECL). A band of ~70 kDa is detected. Immunoprecipitation: use at 1-2ug/ml Immunohistochemistry/Immunocyto- chemistry: use at 2 ug/ml User should determine optimal concentrations for their applications. Positive control: Heat-shocked HeLa cell lysate.
宿主 :
小鼠
格式 :
纯化
纯化 :
Purified by Protein G affinity chromatography.
抗体亚型 :
IgG1
克隆编号 :
3A3
免疫原 :
重组人类Hsp70在大肠杆菌中表达
研究领域 :
加热休克&压力蛋白
配方 :
PBS, pH值7.4和50% 甘油
缓冲液 :
磷酸盐缓冲液
缓冲液pH值 :
pH值7.4
缓冲液CRYOPRESERVATIVE :
50% 甘油
靶标背景 :
Hsp70 binds ATP with high affinity and possesses a weak ATPase activity which can be stimulated by binding to unfolded proteins and synthetic peptides. Hsp70 recognizes and binds to nascent polypeptide chains as well as partially folded intermediates of proteins preventing their aggregation and misfolding. The binding of ATP triggers a critical conformational change leading to the release of the bound protein. The ability of Hsp70 to undergo cycles of binding and release from hydrophobic stretches of partially unfolded proteins is the basis for their role in a variety of intracellular functions such as protein synthesis, protein folding and oligomerization and protein transport.
稀释INSTRUCTIONS :
Dilute in PBS or medium which is identical to that used in the assay system.
STORAGE空调 :
This antibody is stable for at least one (1) year at -20C.
特异性 :
This antibody recognizes human, mouse, rat, fish, avian, amphibian, Drosophila, and yeast Hsp70, Hsc70, p75, and, following heat shock, Hsp72.
图像Name_1 :
qed-bioscience-anti-hsp70-antibody-11117-1.png
应用1 :
免疫印迹
图像Name_2 :
qed-bioscience-anti-hsp70-antibody-11117-2.png
应用2 :
免疫印迹
图像Name_3 :
qed-bioscience-anti-hsp70-antibody-11117-3.png
应用3 :
免疫细胞化学
更多信息或购买 :
QED Bioscience 产品网页
公司信息
QED Bioscience
10919 Technology Place, Suite C San Diego, CA 92127
info@qedbio.com
http://www.qedbio.com
800-929-2114 858-675-2405
公司总部: 美国