抗体

抗Hsp25/Hsp27抗体

11110

100微克

388美元

单克隆

小鼠

未共轭

8A7

人类, 小鼠, 大鼠, 犬, 牛

抗Hsp25/Hsp27抗体

抗Hsp25/Hsp27抗体(11110)

抗体

抗Hsp25/Hsp27克隆8A7小鼠单克隆抗体

Hsp25/Hsp27克隆8A7

人类, 小鼠, 大鼠, 牛, 狗, 豚鼠, 仓鼠

Hsp25/Hsp27

P04792

Cytoplasm , Nucleus , Cytoplasm, cytoskeleton, spindle

热休克蛋白乙1

HspB1, 28 kDa heat shock protein, Estrogen-regulated 24 kDa protein, Heat shock 27 kDa protein, HSP 27, Stress-responsive protein 27, SRP27

HSPB1

NP_001532.1

单克隆

液体

Lot Specific

100微克

388美元

271.6

11110

免疫印迹, IF

Immunoblotting: use at 0.2ug/ml (ECL). A band of 25-27 kDa is detected. Immunofluorescence: use at 5ug/ml. Positive control: HeLa cell lysate

小鼠

纯化

经免疫亲和层析纯化

IgG1

8A7

Hsp27肽

加热休克&压力蛋白

PBS, pH值7.4

磷酸盐缓冲液

pH值7.4

Hsp25 is the mouse homologue of human Hsp27, a member of the small heat shock protein family comprised of proteins of ~15->30kDa. Oligomers of Hsp27 consist of as many as 8-40 Hsp27 monomers; large oligomers have high chaperone activity whereas dimers have no chaperone activity. Hsp27 is localized to the cytoplasm of unstressed cells but redistributes to the nucleus in response to stress where it may stabilize DNA and/or the nuclear membrane. It can be rapidly phosphorylated in response to physiological stimuli, and, therefore, has been suggested as an important intermediate in second messenger- mediated signaling pathways. Hsp27 appears to be involved in cell differentiation and may play a role in termination of cell growth.

Small heat shock protein which functions as a molecular chaperone probably maintaining denatured proteins in a folding-competent state (PubMed:10383393, PubMed:20178975). Plays a role in stress resistance and actin organization (PubMed:19166925). Through its molecular chaperone activity may regulate numerous biological processes including the phosphorylation and the axonal transport of neurofilament proteins (PubMed:23728742). {PubMed:10383393, PubMed:19166925, PubMed:20178975, PubMed:23728742}.

Dilute in PBS or medium which is identical to that used in the assay system.

This antibody is stable for at least one (1) year at -20C. Avoid repeated freezing and thawing.

This antibody recognizes human, mouse, rat, bovine, canine, guinea pig, and hamster Hsp 25/27. Some cross- reactivity with alpha B crystallin.

qed-bioscience-anti-hsp25-hsp27-antibody-11110-1.png

免疫组化

qed-bioscience-anti-hsp25-hsp27-antibody-11110-2.png

免疫组化

qed-bioscience-anti-hsp25-hsp27-antibody-11110-3.png

免疫组化

qed-bioscience-anti-hsp25-hsp27-antibody-11110-4.png

Immunohistochemistry analysis using Mouse Anti-Hsp27 Monoclonal Antibody, Clone 8A7 (11110). Tissue: embryo somites. Species: Rat. Primary Antibody: Mouse Anti-Hsp27 Monoclonal Antibody (11110) at 1:1000. Secondary Antibody: FITC Goat Anti-Mouse (green). Counterstain: Rhodamine-phalloidin labeled actin (red). Investigation at 11 days gestation. Courtesy of: Mike Welsh, Umich.

免疫组化

qed-bioscience-anti-hsp25-hsp27-antibody-11110-5.png

Western Blot analysis of Rat Lung tissue lysates showing detection of Hsp27 protein using Mouse Anti-Hsp27 Monoclonal Antibody, Clone 8A7 (11110). Load: 15 µg. Block: 5% blocking solution. Primary Antibody: Mouse Anti-Hsp27 Monoclonal Antibody (11110) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse: HRP for 1 hour at RT.

免疫印迹