抗体

抗Hsp70抗体

11108

100微升

436美元

多克隆

domestic rabbit

未共轭

人类, 小鼠, 大鼠, 牛

抗Hsp70抗体

抗Hsp70抗体(11108)

抗体

抗Hsp70兔多克隆抗体

Hsp70

Human, mouse, rat, beluga, bovine, canine, fish, guinea pig, hamster, mokey, porcine, ovine, coral

Hsp70

P08107

NP_005336.3

多克隆

液体

Lot Specific

100微升

436美元

305.2

11108

免疫印迹, 免疫沉淀

Immunoblotting: use at 1:10,000- 1:25,000 (ECL). A band of ~70 kDa is detected. Immunoprecipitation: use at 1:100 dilution Positive control: HeLa cell lysate.

兔

血清

全长Hsp70蛋白

加热休克&压力蛋白

Whole rabbit serum.

Whole rabbit serum.

Hsp70 binds ATP with high affinity and possesses a weak ATPase activity which can be stimulated by binding to unfolded proteins and synthetic peptides. Hsp70 recognizes and binds to nascent polypeptide chains as well as partially folded intermediates of proteins preventing their aggregation and misfolding. The binding of ATP triggers a critical conformational change leading to the release of the bound protein. The ability of Hsp70 to undergo cycles of binding and release from hydrophobic stretches of partially unfolded proteins is the basis for their role in a variety of intracellular functions such as protein synthesis, protein folding and oligomerization and protein transport.

Dilute in PBS or medium which is identical to that used in the assay system.

This antibody is stable for at least one (1) year at -20C. Avoid repeated freezing and thawing.

This antibody recognizes human, mouse, rat, beluga, bovine, canine, fish, guinea pig, hamster, monkey, porcine, ovine, and coral Hsp70.

qed-bioscience-anti-hsp70-antibody-11108-1.png

免疫细胞化学

qed-bioscience-anti-hsp70-antibody-11108-2.png

免疫组化

qed-bioscience-anti-hsp70-antibody-11108-3.png

免疫印迹

qed-bioscience-anti-hsp70-antibody-11108-4.png

Immunohistochemistry analysis using Rabbit Anti-HSP70 Polyclonal Antibody (11108). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative Solution. Primary Antibody: Rabbit Anti-HSP70 Polyclonal Antibody (11108) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:50 for 1 hour at RT. Localization: Cytoplasm.

免疫组化

qed-bioscience-anti-hsp70-antibody-11108-5.png

Western blot analysis of Mouse Pam212 cells showing detection of HSP70 protein using Rabbit Anti-HSP70 Polyclonal Antibody (11108). Load: 15 µgprotein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Rabbit Anti-HSP70 Polyclonal Antibody (11108) at 1:1000 for 2 hours at RT. Secondary Antibody: Donkey Anti-Rabbit IgG: HRP for 1 hour at RT.

免疫印迹

qed-bioscience-anti-hsp70-antibody-11108-6.png

Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Hsp70 Polyclonal Antibody (11108). Tissue: Heat Shocked Cervical cancer cell line (HeLa). Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-Hsp70 Polyclonal Antibody (11108) at 1:100 for 12 hours at 4°C. Secondary Antibody: APC Goat Anti-Rabbit (red) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cytoplasm. Magnification: 20x. (A) DAPI (blue) nuclear stain. (B) Anti-Hsp70 Antibody. (C) Composite. Heat Shocked at 42°C for 1h.

免疫细胞化学

qed-bioscience-anti-hsp70-antibody-11108-7.png

Immunohistochemistry analysis using Rabbit Anti-HSP70 Polyclonal Antibody (11108). Tissue: Inflamed colon. Species: Mouse. Fixation: Formalin. Primary Antibody: Rabbit Anti-HSP70 Polyclonal Antibody (11108) at 1:1000 for 12 hours at 4°C. Secondary Antibody: Biotin Goat Anti-Rabbit at 1:2000 for 1 hour at RT. Counterstain: Methyl Green at 200uL for 2 min at RT.

免疫组化