产品简要
公司名称 :
QED Bioscience
产品类型 :
抗体
产品名称 :
抗HSF2抗体
目录 :
11105
规格 :
100微克
价格 :
374美元
克隆性 :
单克隆
宿主 :
大鼠
共轭标签 :
未共轭
克隆名称 :
3-E2
反应物种 :
人类, 小鼠, 大鼠, 犬, 牛
更多信息或购买 :
图像
图像 1 :
Immunocytochemistry/Immunofluorescence analysis using Rat Anti-HSF2 Monoclonal Antibody, Clone 3E2 (11105). Tissue: Heat Shocked cervical cancer cells (HeLa). Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rat Anti-HSF2 Monoclonal Antibody (11105) at 1:100 for 12 hours at 4°C. Secondary Antibody: R-PE Goat Anti-Rat (yellow) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Diffuse nuclear and cytoplasmic staining. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-HSF2 Antibody. (C) Composite. Heat Shocked at 42°C for 1h.
图像 2 :
Immunocytochemistry/Immunofluorescence analysis using Rat Anti-HSF2 Monoclonal Antibody, Clone 3E2 (11105). Tissue: Heat Shocked cervical cancer cells (HeLa). Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rat Anti-HSF2 Monoclonal Antibody (11105) at 1:100 for 12 hours at 4°C. Secondary Antibody: APC Goat Anti-Rat (red) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Diffuse nuclear and cytoplasmic staining. Magnification: 20x. (A) DAPI (blue) nuclear stain. (B) Anti-HSF2 Antibody. (C) Composite. Heat Shocked at 42°C for 1h.
图像 3 :
Western Blot analysis of Human K562 cell lysates showing detection of HSF2 protein using Rat Anti-HSF2 Monoclonal Antibody, Clone 3E2 (11105). Primary Antibody: Rat Anti-HSF2 Monoclonal Antibody (11105) at 1:1000. Cells transiently transfected with control, HSF1 or HSF2 shRNA constructs. Courtesy of: Lea Sistonen, Abo Akademi University, Finland.
产品信息
目录号 :
11105
产品名称 :
抗HSF2抗体
NAME+SKU :
抗HSF2抗体(11105)
产品_TYPE :
抗体
描述 :
抗HSF2大鼠单克隆抗体
SPECIFICITY SHORT :
HSF2
物种活性 :
Human, mouse, rat, rabbit, bovine, canine, porcine, guinea pig, hamster, sheep, monkey
靶标名 :
HSF2
UniProt数据库 :
P38533
LOCATION :
Cytoplasm. Nucleus. Note=Cytoplasmic during normal growth and moves to the nucleus upon activation.
蛋白名 :
Heat shock factor protein 2
登录号 :
NP_001129036.1
克隆性 :
单克隆
形式 :
液体
浓度 :
Lot Specific
规格1 :
100微克
标价规格1 :
374美元
DISTIRBUTOR价格 :
261.8
SKU号规格1 :
11105
应用 :
免疫印迹, 免疫沉淀, 免疫组化
应用INSTRUCTIONS :
Immunoblotting: use at 1-5ug/ml. A band of ~69 kDa is detected.
Immunohistochemistry / Immuno- fluorescence: use at 1-5 ug/ml.
Immunoprecipitation: use 1-2ug per 100- 500ug total protein.
These are recommended concentrations. User should determine optimal concentrations for their application.
Positive control: Heat-shocked HeLa cell lysate, K562 cell nuclear extract, human pancreatic tumor cells.
宿主 :
大鼠
格式 :
纯化
纯化 :
经免疫亲和层析纯化
抗体亚型 :
IgG1
克隆编号 :
3-E2
免疫原 :
重组全长小鼠HSF2
研究领域 :
加热休克&压力蛋白
配方 :
PBS, pH值7.4
缓冲液 :
磷酸盐缓冲液
缓冲液pH值 :
pH值7.4
靶标背景 :
Heat shock factor 1 (HSF1) is a heat shock transcription factor that activates the transcription of genes encoding products required for protein folding, processing, targeting, degradation, and function. Up- regulation of expression of heat shock proteins in response to stress occurs at the level of transcription through a heat shock element and a HSF transcription factor. Amino acid sequences for most HSFs are highly conserved. A DNA binding domain is at the N-terminus, hydrophobic repeats (essential to the formation of active trimers) are adjacent to this binding domain, and another short hydrophobic repeat (necessary for suppression of trimerization) occurs toward the C-terminus. HSF2 exists as two isoforms, the alpha form being more transcriptionally active than the smaller beta form. Various experiments have suggested that HSF2 may play roles in differentiation and development.
FUNCTION :
DNA-binding protein that specifically binds heat shock promoter elements (HSE) and activates transcription. In higher eukaryotes, HSF is unable to bind to the HSE unless the cells are heat shocked. HSF2 is expressed in a form that binds DNA constitutively but loses DNA binding by incubation at greater than 41 degrees C.
稀释INSTRUCTIONS :
Dilute in PBS or medium which is identical to that used in the assay system.
STORAGE空调 :
This antibody is stable for at least one (1) year at -20C. Avoid repeated freezing and thawing.
特异性 :
This antibody recognizes HSF2 in heat- shocked cell lysates of human, mouse, rat, rabbit, bovine, canine, porcine, guinea pig, hamster, sheep, and monkey.
图像Name_1 :
qed-bioscience-anti-hsf2-antibody-11105-1.png
应用1 :
免疫细胞化学
图像Name_2 :
qed-bioscience-anti-hsf2-antibody-11105-2.png
应用2 :
免疫细胞化学
图像Name_3 :
qed-bioscience-anti-hsf2-antibody-11105-3.png
应用3 :
免疫印迹
更多信息或购买 :
公司信息
QED Bioscience
10919 Technology Place, Suite C San Diego, CA 92127
info@qedbio.com
http://www.qedbio.com800-929-2114 858-675-2405
公司总部: 美国
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