抗体

抗Hsp60抗体

11100

100微克

239美元

单克隆

小鼠

未共轭

LK-1

人类, 小鼠, 大鼠, 犬, 鸡, 牛

抗Hsp60抗体

抗Hsp60抗体(11100)

抗体

抗Hsp60小鼠单克隆抗体

Hsp60

Human, mouse, rat, rabbit, bovine, canine, porcine, ovine, guinea pig, hamster, chicken, monkey, Xenopus, Drosophila

Hsp60

P10809

Mitochondrion matrix.

60 kDa heat shock protein, mitochondrial

EC 5.6.1.7, 60 kDa chaperonin, Chaperonin 60, CPN60, Heat shock protein 60, HSP-60, Hsp60, HuCHA60, Mitochondrial matrix protein P1, P60 lymphocyte protein

HSPD1

NP_002147.2

单克隆

液体

Lot Specific

100微克

239美元

167.3

11100

免疫印迹, 免疫沉淀, 流式细胞仪

Immunoblotting: use at 0.1-1ug/ml. A band of ~60 kDa is detected. Immunoprecipitation: use at 12.5ug/ml Flow cytometry: use at 10ug/ml These are recommended concentrations. User should determine optimal concentrations for their application. Positive control: Heat-shocked HeLa cell lysate.

小鼠

纯化

经免疫亲和层析纯化

IgG1

LK-1

重组人类Hsp60在大肠杆菌中表达

加热休克&压力蛋白

PBS, pH值7.4

磷酸盐缓冲液

pH值7.4

Hsp60 is an abundant protein synthesized constitutively in various cell types that is induced to higher concentrations after cell shock. It is present in mitochondria of many mammalian species and has highly similar counterparts in bacteria and plants (where it is localized to chloroplasts). In general, Hsp60 proteins are present in high concentrations, are induced in response to environmental stresses (such as heat shock) are homo-oligomeric structures of 7 or 14 subunits that dissociate reversibly in the presence of Mg2+ and ATP, have ATPase activity, and play a role in folding and assembly of oligomeric protein structures. Hsp60 has been linked to Alzheimer’s disease, coronary artery disease, multiple sclerosis, diabetes, and other autoimmune diseases.

Chaperonin implicated in mitochondrial protein import and macromolecular assembly. Together with Hsp10, facilitates the correct folding of imported proteins. May also prevent misfolding and promote the refolding and proper assembly of unfolded polypeptides generated under stress conditions in the mitochondrial matrix (PubMed:1346131, PubMed:11422376). The functional units of these chaperonins consist of heptameric rings of the large subunit Hsp60, which function as a back-to-back double ring. In a cyclic reaction, Hsp60 ring complexes bind one unfolded substrate protein per ring, followed by the binding of ATP and association with 2 heptameric rings of the co-chaperonin Hsp10. This leads to sequestration of the substrate protein in the inner cavity of Hsp60 where, for a certain period of time, it can fold undisturbed by other cell components. Synchronous hydrolysis of ATP in all Hsp60 subunits results in the dissociation of the chaperonin rings and the release of ADP and the folded substrate protein (Probable). {PubMed:11422376, PubMed:1346131, PubMed:25918392}.

Dilute in PBS or medium which is identical to that used in the assay system.

This antibody is stable for at least one (1) year at -20C. Avoid repeated freezing and thawing.

This antibody recognizes human, mouse, rat, rabbit, bovine, canine, porcine, ovine, guinea pig, hamster, chicken, monkey, Xenopus, and Drosophila Hsp60 (60 kDa). It does not cross-react with bacterial or yeast Hsp60. The epitope recognized is within aa 383-447 of human Hsp60.

qed-bioscience-anti-hsp60-antibody-11100-1.png

免疫细胞化学

qed-bioscience-anti-hsp60-antibody-11100-2.png

免疫细胞化学

qed-bioscience-anti-hsp60-antibody-11100-3.png

免疫组化

qed-bioscience-anti-hsp60-antibody-11100-4.png

Immunohistochemistry analysis using Mouse Anti-Hsp60 Monoclonal Antibody, Clone LK-1 (11100). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-Hsp60 Monoclonal Antibody (11100) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Epidermis.

免疫组化

qed-bioscience-anti-hsp60-antibody-11100-5.png

Western Blot analysis of Human Cell line lysates showing detection of Hsp60 protein using Mouse Anti-Hsp60 Monoclonal Antibody, Clone LK-1 (11100). Load: 15 µg. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Hsp60 Monoclonal Antibody (11100) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.

免疫印迹