抗体

抗Hsp70/Hsc70抗体

11099

100微克

293美元

单克隆

小鼠

未共轭

BB70

人类, 小鼠, 大鼠, 犬, 鸡, 牛

抗Hsp70/Hsc70抗体

抗Hsp70/Hsc70抗体(11099)

抗体

抗Hsp70/Hsc70小鼠单克隆抗体

Hsp70/Hsc70

Human, mouse, rat, rabbit, monkey, hamster, guinea pig, dog, bovine, sheep, pig, chicken, yeast, fish, Xenopus

Hsp70/Hsc70

P08106

Heat shock 70 kDa protein

HSP70

NP_001006686.1

单克隆

液体

Lot Specific

100微克

293美元

205.1

11099

免疫印迹, 免疫组化

Immunoblotting: use at 1ug/ml. Immunohistochemistry: use at 1-5ug/ml. These are recommended concentrations. Endusers should determine optimal concentrations for their applications.

小鼠

纯化

Purified by Protein G affinity chromatography.

IgG2a

BB70

Chicken Hsp70/Hsp90 complex.

加热休克&压力蛋白

PBS, pH值7.4, 0.1% 叠氮化钠

磷酸盐缓冲液

pH值7.4

0.1% 叠氮化钠

Members of the Hsp70 family are molecular chaperones that function in protein folding, transport, maturation and degradation by binding to nascent polypeptide chains as well as partially folded protein intermediates and preventing their aggregation and misfolding. The 70kDa heat shock cognate protein, Hsc70, is closely related biochemically and biologically to Hsp70.

Molecular chaperone implicated in a wide variety of cellular processes, including protection of the proteome from stress, folding and transport of newly synthesized polypeptides, activation of proteolysis of misfolded proteins and the formation and dissociation of protein complexes. Plays a pivotal role in the protein quality control system, ensuring the correct folding of proteins, the re-folding of misfolded proteins and controlling the targeting of proteins for subsequent degradation. This is achieved through cycles of ATP binding, ATP hydrolysis and ADP release, mediated by co-chaperones. The affinity for polypeptides is regulated by its nucleotide bound state. In the ATP-bound form, it has a low affinity for substrate proteins. However, upon hydrolysis of the ATP to ADP, it undergoes a conformational change that increases its affinity for substrate proteins. It goes through repeated cycles of ATP hydrolysis and nucleotide exchange, which permits cycles of substrate binding and release. {UniProtKB:P0DMV8}.

Dilute in PBS or medium that is identical to that used in the assay system.

This antibody is stable for 1 month at 4°C or one year at -20°C. Avoid multiple freeze-thaw cycles.

This antibody detects 72 and 73kDa proteins corresponding to the predicted molecular masses of Hsp70 and Hsc70, respectively, on immunoblots of human, mouse, rat, rabbit, monkey, hamster, guinea pig, dog, bovine, sheep, pig, chicken yeast, fish, and Xenopus samples. This antibody recognizes the inducible and constitutive forms of Hsp70 and does not cross-react with Hsp90.

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免疫细胞化学

qed-bioscience-anti-hsp70-hsc70-antibody-11099-2.png

免疫组化

qed-bioscience-anti-hsp70-hsc70-antibody-11099-3.png

免疫组化

qed-bioscience-anti-hsp70-hsc70-antibody-11099-4.png

Immunohistochemistry analysis using Mouse Anti-Hsp70 Monoclonal Antibody, Clone BB70 (11099). Tissue: hepatocytes. Species: Rat. Fixation: Paraffin Embedded. Primary Antibody: Mouse Anti-Hsp70 Monoclonal Antibody (11099) at 1:200. Liver sections were paraffin embedded. First pictures in series show two hours after exposure to stress, the second shows the control. Courtesy of: G. Matic, University of Belgrade, Serbia.

免疫组化

qed-bioscience-anti-hsp70-hsc70-antibody-11099-5.png

Western Blot analysis of Bovine MDBK cell lysates showing detection of Hsp70 protein using Mouse Anti-Hsp70 Monoclonal Antibody, Clone BB70 (11099). Primary Antibody: Mouse Anti-Hsp70 Monoclonal Antibody (11099) at 1:1000.

免疫印迹

qed-bioscience-anti-hsp70-hsc70-antibody-11099-6.png

Western Blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of Hsp70 protein using Mouse Anti-Hsp70 Monoclonal Antibody, Clone BB70 (11099). Primary Antibody: Mouse Anti-Hsp70 Monoclonal Antibody (11099) at 1:1000. Secondary Antibody: HRP Goat Anti-Rat.

免疫印迹