产品简要
公司名称 :
QED Bioscience
产品类型 :
抗体
产品名称 :
抗Grp94抗体
目录 :
11097
规格 :
100微克
价格 :
293美元
克隆性 :
单克隆
宿主 :
大鼠
共轭标签 :
未共轭
克隆名称 :
9G10
反应物种 :
人类, 小鼠, 大鼠, 犬, 鸡, 牛
更多信息或购买 :
图像
图像 1 :
Immunocytochemistry/Immunofluorescence analysis using Rat Anti-GRP94 Monoclonal Antibody, Clone 9G10 (11097). Tissue: Heat Shocked cervical cancer cells (HeLa). Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rat Anti-GRP94 Monoclonal Antibody (11097) at 1:100 for 12 hours at 4°C. Secondary Antibody: R-PE Goat Anti-Rat (yellow) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Endoplasmic reticulum lumen. Melanosome. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-GRP94 Antibody. (C) Composite. Heat Shocked at 42°C for 1h.
图像 2 :
Immunocytochemistry/Immunofluorescence analysis using Rat Anti-GRP94 Monoclonal Antibody, Clone 9G10 (11097). Tissue: Heat Shocked cervical cancer cells (HeLa). Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rat Anti-GRP94 Monoclonal Antibody (11097) at 1:100 for 12 hours at 4°C. Secondary Antibody: APC Goat Anti-Rat (red) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Endoplasmic reticulum lumen. Melanosome. Magnification: 20x. (A) DAPI (blue) nuclear stain. (B) Anti-GRP94 Antibody. (C) Composite. Heat Shocked at 42°C for 1h.
图像 3 :
Western Blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of GRP94 protein using Rat Anti-GRP94 Monoclonal Antibody, Clone 9G10 (11097). Primary Antibody: Rat Anti-GRP94 Monoclonal Antibody (11097) at 1:1000. Secondary Antibody: HRP Goat Anti-Rat.
产品信息
目录号 :
11097
产品名称 :
抗Grp94抗体
NAME+SKU :
抗Grp94抗体(11097)
产品_TYPE :
抗体
描述 :
抗Grp94大鼠单克隆抗体
SPECIFICITY SHORT :
Grp94
物种活性 :
Human, mouse, rat, rabbit, monkey, hamster, guinea pig, dog, bovine, sheep, pig, chicken, Xenopus
靶标名 :
Grp94
UniProt数据库 :
P14625
LOCATION :
Endoplasmic reticulum lumen , Sarcoplasmic reticulum lumen , Melanosome
蛋白名 :
Endoplasmin
别称 :
94 kDa glucose-regulated protein, GRP-94, Heat shock protein 90 kDa beta member 1, Tumor rejection antigen 1, gp96 homolog
基因名称 :
HSP90B1
登录号 :
NP_003290.1
克隆性 :
单克隆
形式 :
液体
浓度 :
Lot Specific
规格1 :
100微克
标价规格1 :
293美元
DISTIRBUTOR价格 :
205.1
SKU号规格1 :
11097
应用 :
免疫印迹, 免疫沉淀, 免疫组化
应用INSTRUCTIONS :
Immunoblotting: use at 0.5-1ug/ml. A band of ~98 kDa is detected.
Immunohistochemistry: use at 2-30ug/ml.
Immunoprecipitation: 10ug/ml.
These are recommended concentrations. User should determine optimal concentrations for their application.
Positive control: HeLa cell lysate.
宿主 :
大鼠
格式 :
纯化
纯化 :
经免疫亲和层析纯化
抗体亚型 :
IgG2a
克隆编号 :
9G10
免疫原 :
Purified Grp94 isolated from chicken oviducts.
研究领域 :
加热休克&压力蛋白
配方 :
PBS, pH值7.4
缓冲液 :
磷酸盐缓冲液
缓冲液pH值 :
pH值7.4
靶标背景 :
Glucose-regulated protein 94 (Grp94) is an endoplasmic reticulum luminal stress protein which belongs to the Hsp90 family of molecular chaperones. The sequence of Grp94 includes the COOH-terminal tetrapeptide Lys-Asp-Glu-Leu (KDEL) which is a signal sequence that causes proteins to be retained in the pre-Golgi compartments. Expression of Grp94 is up-regulated by stress conditions such as glucose starvation and heat shock, and it functions in intracellular trafficking of peptides from the extracellular space to the MHC class I antigen processing pathway of antigen- presenting cells.
FUNCTION :
Molecular chaperone that functions in the processing and transport of secreted proteins (By similarity). When associated with CNPY3, required for proper folding of Toll-like receptors (By similarity). Functions in endoplasmic reticulum associated degradation (ERAD) (PubMed:18264092). Has ATPase activity (By similarity). May participate in the unfolding of cytosolic leaderless cargos (lacking the secretion signal sequence) such as the interleukin 1/IL-1 to facilitate their translocation into the ERGIC (endoplasmic reticulum-Golgi intermediate compartment) and secretion; the translocation process is mediated by the cargo receptor TMED10 (PubMed:32272059). {UniProtKB:P08113, PubMed:18264092, PubMed:32272059}.
稀释INSTRUCTIONS :
Dilute in PBS or medium which is identical to that used in the assay system.
STORAGE空调 :
This antibody is stable for at least one (1) year at -20C. Avoid multiple freeze-thaw cycles.
特异性 :
This antibody recognizes an ~98 kDa protein corresponding to the predicted molecular mass of Grp94 on SDS-PAGE immunoblots in human, mouse, rat, rabbit, monkey, hamster, guinea pig, dog, bovine, sheep, pig, chicken and Xenopus.
图像Name_1 :
qed-bioscience-anti-grp94-antibody-11097-1.png
应用1 :
免疫细胞化学
图像Name_2 :
qed-bioscience-anti-grp94-antibody-11097-2.png
应用2 :
免疫细胞化学
图像Name_3 :
qed-bioscience-anti-grp94-antibody-11097-3.png
应用3 :
免疫印迹
更多信息或购买 :
公司信息
QED Bioscience
10919 Technology Place, Suite C San Diego, CA 92127
info@qedbio.com
http://www.qedbio.com800-929-2114 858-675-2405
公司总部: 美国
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