抗体

抗Hsp70抗体

11096

100微克

293美元

单克隆

小鼠

未共轭

C92F3A-5

人类, 小鼠, 大鼠, 牛

抗Hsp70抗体

抗Hsp70抗体(11096)

抗体

抗Hsp70小鼠单克隆抗体

Hsp70

Human, mouse, rat, rabbit, monkey, guinea pig, hamster, chicken, bovine, canine, ovine, porcine, fish, Drosophila, C. elegans

Hsp70

P08107

NP_005336.3

单克隆

液体

Lot Specific

100微克

293美元

205.1

11096

免疫印迹, 免疫沉淀, 免疫组化, 酶联免疫吸附测定, 流式细胞仪

Immunoblotting: 1ug/ml. A band of 70kDa is detected. Immunoblotting of Hsp70 in cell lysates from human cancer cell lines with #11096. Immunohistochemistry: 1-10ug/ml Immunohistochemical staining of inflammatory cells in human colon tissue with #11096. Immunoprecipitation: 1-10ug/ml ELISA: 1-10ug/ml Flow cytometry: 10ug/ml These are recommended concentrations. User should determine optimal concentrations for their application. Positive control: HeLa cell lysate.

小鼠

纯化

经免疫亲和层析纯化

IgG1

C92F3A-5

人类Hsp70纯化来自HeLa细胞

加热休克&压力蛋白

PBS含0.1% 叠氮化钠

磷酸盐缓冲液

0.1% 叠氮化钠

Hsp70 genes, members of a multigene family, encode heat-inducible 70kDa heat- shock proteins that function as molecular chaperones. They have been identified in most organelles of eukaryotic cells as well as in bacteria. The N-terminus of Hsp70 binds ATP with high affinity and the C- terminus binds proteins and polypeptides. Hsp70s bind nascent polypeptides as well as partially folded intermediates of proteins, thus preventing their aggregation and/or misfolding. Binding of ATP triggers a conformational change that results in the release of bound protein. Hsp70s function in protein synthesis, protein folding and oligomerization, and protein transport.

Dilute in PBS or medium which is identical to that used in the assay system.

This antibody is stable for at least one (1) year at 4C. Precipitation may occur upon freezing and thawing.

This antibody recognizes Hsp70 of human, mouse, rat, rabbit, monkey, guinea pig, hamster, chicken, bovine, canine, ovine, porcine, fish, Drosophila, and C. elegans and does not cross-react with Hsc70. The epitope is in the region of amino acids 436- 503 of human Hsp70.

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免疫细胞化学

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免疫组化

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免疫组化

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Western Blot analysis of Human cell lysates from various cell lines showing detection of Hsp70 protein using Mouse Anti-Hsp70 Monoclonal Antibody, Clone C92F3A-5 (11096). Load: 15 µg. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Hsp70 Monoclonal Antibody (11096) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.

免疫印迹

qed-bioscience-anti-hsp70-antibody-11096-5.png

Fluorescence Activated Cell Sorting analysis using Mouse Anti-Hsp70: FITC Monoclonal Antibody, Clone C92F3A-5 (11096). Tissue: Heat Shocked CD3+ CD8+ T cells . Species: Mouse. Primary Antibody: Mouse Anti-Hsp70: FITC Monoclonal Antibody (11096) at 1:1000. Courtesy of: Cheryl Cameron, Vaccine and Gene Therapy Instit. Florida.

流式细胞仪