蛋白

Recombinant human 14-3-3 epsilon protein

YWE0701

20微克

105美元

Recombinant human 14-3-3 epsilon protein

1-255aa

YWE0701

NP_006752.1

The 14-3-3 family of proteins plays a key regulatory role in signal transduction, checkpoint control, apoptotic and nutrient-sensing pathways. 14-3-3 proteins are highly conserved and ubiquitously expressed. There are at least seven isoforms, beta, gamma, epsilon, sigma, zeta, tau and eta that have been identified in mammals. The 14-3-3 epsilon, a subtype of the 14-3-3 family of proteins, was thought to be brain and neuron-specific. It has been shown to interact with CDC25 phosphatases, RAF1 and IRS1 proteins, suggesting its role in diverse biochemical activities related to signal transduction, such as cell division and regulation of insulin sensitivity. It has also been implicated in the pathogenesis of small cell lung cancer. Recombinant human 14-3-3 epsilon was expressed in E. coli and purified by using conventional chromatography techniques.

YWHAE, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein epsilon, 14-3-3E, HEL2, KCIP-1, MDCR, MDS

1毫克/毫升(由布拉德福德Bradford检测确定)

液体。 20mM三羟甲基氨基甲烷氯化氢缓冲液(pH值7.5)

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Oriente F, et al.(2005) J Biol Chem. 280(49):40642-9.;; Conklin D, et al.(1995) PNAS. 92(17):7892-6;;

> 95% by SDS-PAGE

十二烷基磺酸钠-PAGE

人类

信号转导

29 kDa (255aa) confirmed by MALDI-TOF

MDDREDLVYQ AKLAEQAERY DEMVESMKKV AGMDVELTVE ERNLLSVAYK NVIGARRASW RIISSIEQKE ENKGGEDKLK MIREYRQMVE TELKLICCDI LDVLDKHLIP AANTGESKVF YYKMKGDYHR YLAEFATGND RKEAAENSLV AYKAASDIAM TELPPTHPIR LGLALNFSVF YYEILNSPDR ACRLAKAAFD DAIAELDTLS EESYKDSTLI MQLLRDNLTL WTSDMQGDGE EQNKEALQDV EDENQ

Non-Tagged

大肠杆菌

重组蛋白

P62258

1433 epsilon, Tyrosine 3-monooxygenase/ tryptophan 5-monooxygenase activation protein epsilon polypeptide, YWHAE, MDS, MDCR, KCIP-1, FLJ45465, 14-3-3 epsilon, Tyrosine 3/tryptophan 5-monooxygenase activation protein, epsilon polypeptide, Tyrosine 3-monooxygenase/ tryptophan 5-monooxygenase activation protein, epsilon polypeptide 14 3 3 E, 14 3 3E, KCIP 1, KCIP1, Mitochondrial import stimulation factor L subunit, Protein kinase C inhibitor protein1, Tyrosine 3/tryptophan 5 monooxygenase activation protein epsilon polypeptide.,YWE0701-10ug,YWE0701-20ug,YWE0701-50ug,YWE0701-100ug,YWE0701-250ug,YWE0701-500ug,YWE0701-1mg,YWE0701-10,YWE0701-20,YWE0701-50,YWE0701-100,YWE0701-250,YWE0701-500,YWE0701-1

Wu JS, et al. Ligand-activated peroxisome proliferator-activated receptor-gamma protects against ischemic cerebral infarction and neuronal apoptosis by 14-3-3 epsilon upregulation. (Circulation. 2009){https://pubmed.ncbi.nlm.nih.gov/19221220/}