抗体

人类KRT5抗体

ATGA0368

50微升

170美元

单克隆

小鼠

未共轭

AT12C4

免疫印迹, 酶联免疫吸附测定, 免疫组化, 免疫细胞化学, 流式细胞仪

人类KRT5抗体

ATGA0368

NP_000415

Keratin 5, also known as KRT5, K5, or CK5, dimerizes with keratin 14 and forms the intermediate filaments (IF) that make up the cytoskeleton of basal epithelial cells. This protein is involved in several diseases including epidermolysis bullosa simplex and breast and lung cancers. Keratin 5 (and K14) are expressed primarily in basal keratinocytes in the epidermis, specifically in the stratified epithelium lining the skin and digestive tract. Keratin intermediate filaments make up the cytoskeletal scaffold within epithelial cells, which contributes to the cell architecture and provides the cells with the ability to withstand mechanical, and non-mechanical, stresses. K5/K14 keratin pairs are able to undergo extensive bundling due to the non-helical tail of K15 acting as a weak cross-linker at the intermediate filament surface. This bundling increase the elasticity, and therefore the mechanical resilience, of the intermediate filaments.

角蛋白5, CK5, DDD, K5, KRT5A

1mg/ml (determined by BCA assay)

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Lersch R., et al. (1988) Molecular and Cellular Biology. 8(1): 486-493.;; Eckert RL., et al. (1988) Dna. 7(5): 337-345.;; Atkinson SD., et al. (2011) J Invest Dermatol. 131(10): 2079-2086.;; van de Rijn M., et al. (2002) Am J Pathol. 161(6): 1991-1996.

小鼠

IgG2a kappa

重组人类KRT5(1-590aa)纯化来自大肠杆菌

酶联免疫吸附测定, 免疫印迹, 免疫细胞化学/IF, 免疫组化, 流式细胞仪

ICC/IF analysis of KRT5 in A549 cells. The cell was stained with ATGA0368 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human KRT5 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.;;Lane 1. : HeLa cell lysate;;Lane 2. : A431 cell lysate

人类

AT12C4

The antibody has been tested by ELISA, Western blot, ICC/IF, FACS and IHC analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Flow cytometry analysis of KRT5 in HeLa cells. The cell was stained with ATGA0368 at 2-5ug for 1x10^6cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).

Paraffin embedded sections of human cervical cancer tissue were incubated with anti-human KRT5 (1:200) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB)

人类

信号转导

未共轭

单克隆

By protein-A affinity chromatography

单克隆抗体

P13647

KRT5, ATGA0368-10微克, ATGA0368-20微克, ATGA0368-50微克, ATGA0368-100微克, ATGA0368-250微克, ATGA0368-500微克, ATGA0368-1毫克, ATGA0368-10, ATGA0368-20, ATGA0368-50, ATGA0368-100, ATGA0368-250, ATGA0368-500, ATGA0368-1