抗体

人类eIF-5A1/EIF5A抗体

ATGA0128

50微升

170美元

单克隆

小鼠

未共轭

AT4D1

免疫印迹, 酶联免疫吸附测定, 免疫细胞化学, 流式细胞仪

人类eIF-5A1/EIF5A抗体

ATGA0128

NP_001961

Eukaryotic translation initiation factor 5A (eIF5A) is the only protein Known to contain unusual amino acid formed by the action of deoxyhypusine synthase and deoxyhypusine hydroxylase using spermidine as the substrate. This protein was previously reported to be involved in the first step of peptide bond formation in translation; however more recent work implicates it as a universally conserved translation elongation factor. Modulation of eIF5A has been linked to proliferation and cancer.

Eukaryotic translation initiation factor 5A-1 isoform B, Eukaryotic translation initiation factor 5A, Eukaryotic translation initiation factor 5A-1, eIF-5A-1, Eukaryotic initiation factor 5A isoform 1, eIF-5A, Rev-binding factor, eIF-4D

1mg/ml (determined by BCA assay)

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Saini P., et al. (2009) Nature. 459(7243): 118-21.;; Gosslau A., et al. (2009) J Cell Physiol. 219(2): 485-93.;;

小鼠

IgG1 kappa

重组人类EIF5A(1-154aa)纯化来自大肠杆菌

酶联免疫吸附测定, 免疫印迹, 免疫细胞化学/IF, 流式细胞仪

ICC/IF analysis of EIF5A in Balb/3T3 cells. The cell was stained with ATGA0128 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human EIF5A antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.;;Lane 1.: Jurkat cell lysate;;Lane 2.: 293T cell lysate;;Lane 3.: HeLa cell lysate;;Lane 4.: MCF7 cell lysate;;Lane 5.: NIH-3T3 cell lysate

人类

AT4D1

The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Flow cytometry analysis of EIF5A in Balb/3T3 cell line, staining at 2-5ug for 1x10^6cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).

人类

Epigenomics (Transcription & Translation)

未共轭

单克隆

By protein-G affinity chromatography

单克隆抗体

P63241

eIF5A, ATGA0128-10微克, ATGA0128-20微克, ATGA0128-50微克, ATGA0128-100微克, ATGA0128-250微克, ATGA0128-500微克, ATGA0128-1毫克, ATGA0128-10, ATGA0128-20, ATGA0128-50, ATGA0128-100, ATGA0128-250, ATGA0128-500, ATGA0128-1