抗体

人类OSTF1抗体

ATGA0125

50微升

170美元

单克隆

小鼠

未共轭

AT9G4

免疫印迹, 酶联免疫吸附测定, 免疫细胞化学, 流式细胞仪

人类OSTF1抗体

ATGA0125

AAH07459

Osteoclast-stimulating factor (OSTF1) is an intracellular signaling protein, highly expressed by osteoclasts (OCL). Indirectly, it enhances osteoclast (OCL) formation and bone resorption through a cellular signal transduction cascade by possibly interacting with c-Src or other c-Src-related proteins. OSTF1 contains a short proline-rich N-terminal region, an SH3 domain, three ankyrin repeats and an aspartate-rich C-terminal region, suggesting that it is potentially involved in protein-protein interactions.

Osteoclast stimulating factor 1, SH3P2, OSF, Osteoclast stimulating factor 1

1mg/ml (determined by BCA assay)

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Kurihara N., et al. (2001) J. Biol. Chem. 276(44): 41035-9. ;; Reddy S., et al. (1999) J Cell Physiol. 177(4): 636-45.

小鼠

IgG2a kappa

重组人类OSTF1(1-217aa)纯化来自大肠杆菌

酶联免疫吸附测定, 免疫印迹, 免疫细胞化学/IF, 流式细胞仪

ICC/IF analysis of OSTF1 in MCF7 cells. The cell was stained with ATGA0125 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).ICC/IF analysis of OSTF1 in A431 cells. The cell was stained with ATGA0125 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).{ATGA0125-IF2.jpg}

The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human OSTF1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.;;Lane 1.: HepG2 cell lysate;;Lane 2.: MCF7 cell lysate;;Lane 3.: Jurkat cell lysate;;Lane 4.: LnCap cell lysate The cell lysates (10ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human OSTF1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.;;Lane 1.: 293T cell lysate;;Lane 2.: OSTF1 Transfected 293T cell lysate {ATGA0125-WB2.jpg}

人类

AT9G4

The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Flow cytometry analysis of OSTF1 in HeLa cell line, staining at 2-5ug for 1x10^6cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).

人类

代谢

未共轭

单克隆

By protein-G affinity chromatography

单克隆抗体

Q92882

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