抗体

人类PEDFR/PNPLA2抗体

ATGA0122

50微升

170美元

单克隆

小鼠

未共轭

AT18E6

免疫印迹, 酶联免疫吸附测定, 免疫细胞化学, 流式细胞仪

人类PEDFR/PNPLA2抗体

ATGA0122

NP_065109

Adipose triglyceride lipase (ATGL) is a 504 amino acid protein that is highly expressed in mouse and human adipose tissue. ATGL is catalyzes the initial step in triglyceride hydrolysis in adipocyte lipid droplets and has acylglycerol transacylase activity. Inhibition of ATGL markedly decreases total adipose acyl-hydrolase activity. Thus, ATGL and hormone-sensitive lipase coordinately catabolize stored triglycerides in adipose tissue of mammals.

Adipose triglyceride lipase, Calcium-independent phospholipase A2, Desnutrin IPLA2-zeta, Pigment epithelium-derived factor receptor, TTS2.2, Transport-secretion protein 2, TTS2, ATGL, Patatin-like phospholipase domain-containing protein 2, PNPLA2

1mg/ml (determined by BCA assay)

Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Fischer J, et al., (2007) Nat Genet. 39(1): 28-30.;; Elena S, et al., (2006) EMBO Rep. 7(1): 106-113.;; Zimmermann R, et al., (2004) Science. 306(5700): 1383-1386.

小鼠

IgG2b kappa

重组人类ATGL(30-504aa)纯化来自大肠杆菌

酶联免疫吸附测定, 免疫印迹, 免疫细胞化学/IF, 流式细胞仪

ICC/IF analysis of ATGL in Hep3B cells. The cell was stained with ATGA0122 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).ICC/IF analysis of ATGL in HeLa cells. The cell was stained with ATGA0122 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).{2004010103622287.jpg}ICC/IF analysis of ATGL in A549 cells. The cell was stained with ATGA0122 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).{2004010103476044.jpg} {ATGA0122-IF3b.jpg}

The A431 cell lysate (40ug) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ATGL antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. The mouse adipose tissue lysate (40ug) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ATGL antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. {ATGA0122-WB2.jpg}

人类

AT18E6

The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Flow cytometry analysis of ATGL in Hep3B cells. The cell was stained with ATGA0122 at 2-5ug for 1x10^6cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).Flow cytometry analysis of ATGL in HeLa cells. The cell was stained with ATGA0122 at 2-5ug for 1x10^6cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).{2004010103833302.jpg}

人类

Cardiovascular

ATGA0135 has been replaced with a catalog number ATGA0122.

未共轭

单克隆

By protein-G affinity chromatography

单克隆抗体

Q96AD5

TTS2.2, TTS2, Transport-secretion protein 2, PNPLA2, Pigment epithelium-derived factor receptor, PEDF PNPLA2, Patatin-like phospholipase domain-containing protein 2, DesnutrinIPLA2-zeta, Desnutrin IPLA2-zeta, Calcium-independent phospholipase A2, ATGL, Adipose triglyceride lipase, ATGA0122-10ug, ATGA0122-20ug, ATGA0122-50ug, ATGA0122-100ug, ATGA0122-250ug, ATGA0122-500ug, ATGA0122-1mg, ATGA0122-010, ATGA0122-020, ATGA0122-050, ATGA0122-100, ATGA0122-250, ATGA0122-500, ATGA0122-01M