ELISA/assay

Human Actin, cytoplasmic 2, ACTG1 ELISA Kit

MBS944021

48-Strip-Wells

510美元

酶联免疫吸附试剂盒

Human Actin, cytoplasmic 2, ACTG1 ELISA Kit

肌动蛋白, 丙1

Human Actin; cytoplasmic 2 (ACTG1) ELISA kit; ACT; ACTG; DFNA20; DFNA26; actin; cytoplasmic 2; actin; gamma 1 propeptide; cytoskeletal gamma-actin; actin; gamma 1

actin, cytoplasmic 2; Actin, cytoplasmic 2; actin, cytoplasmic 2; cytoskeletal gamma-actin; epididymis luminal protein 176; actin, gamma 1; Gamma-actinCleaved into the following chain:Actin, cytoplasmic 2, N-terminally processed

ACTG1

ACTG1;ACTG1;ACT;ACTG;BRWS2;DFNA20;DFNA26;HEL-176;ACTB;ACTG

ACTG_HUMAN

人类

375

This assay has high sensitivity and excellent specificity for detection of Human ACTG1. No significant cross-reactivity or interference between Human ACTG1 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, tissue homogenates, Cell lysates. Assay Type: Sandwich. Detection Range: 25 pg/ml-1600 pg/ml. Sensitivity: 6.25 pg/ml

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for ACTG1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ACTG1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ACTG1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ACTG1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

316659409

NP_001186883.1

NM_001199954.1

P63261

41,793 Da

Adherens Junction Pathway (83070); Adherens Junction Pathway (481); Arrhythmogenic Right Ventricular Cardiomyopathy (ARVC) Pathway (117293); Arrhythmogenic Right Ventricular Cardiomyopathy (ARVC) Pathway (116129); Bacterial Invasion Of Epithelial Cells Pathway (149807); Bacterial Invasion Of Epithelial Cells Pathway (148661); Dilated Cardiomyopathy Pathway (121494); Dilated Cardiomyopathy Pathway (121285); Focal Adhesion Pathway (198795); Focal Adhesion Pathway (83067)

Actins are highly conserved proteins that are involved in various types of cell motility, and maintenance of the cytoskeleton. In vertebrates, three main groups of actin isoforms, alpha, beta and gamma have been identified. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins co-exist in most cell types as components of the cytoskeleton, and as mediators of internal cell motility. Actin, gamma 1, encoded by this gene, is a cytoplasmic actin found in non-muscle cells. Mutations in this gene are associated with DFNA20/26, a subtype of autosomal dominant non-syndromic sensorineural progressive hearing loss. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Jan 2011]

ACTG1: Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to 4 others. Belongs to the actin family. Protein type: Motility/polarity/chemotaxis; Cytoskeletal. Chromosomal Location of Human Ortholog: 17q25. Cellular Component: filamentous actin; extracellular space; cytoskeleton; myofibril; membrane; plasma membrane; cytosol; nucleus. Molecular Function: identical protein binding; protein binding; structural constituent of cytoskeleton; ubiquitin protein ligase binding; ATP binding. Biological Process: intercellular junction assembly and maintenance; retinal homeostasis; axon guidance; ephrin receptor signaling pathway; innate immune response; sarcomere organization; vascular endothelial growth factor receptor signaling pathway; cell motility. Disease: Baraitser-winter Syndrome 2; Deafness, Autosomal Dominant 20

48-Strip-Wells

510美元

96-Strip-Wells

725

5x96-Strip-Wells

2565

10x96-Strip-Wells

4800