ELISA/assay

Human Statherin, STATH ELISA Kit

MBS943465

48-Strip-Wells

510美元

酶联免疫吸附试剂盒

Human Statherin, STATH ELISA Kit

statherin

Human Statherin (STATH) ELISA kit; STR; ; statherin

statherin isoform b; Statherin; statherin; statherin

STATH

STATH;STATH;STR

STAT_HUMAN

人类

62

This assay has high sensitivity and excellent specificity for detection of human STATH. No significant cross-reactivity or interference between human STATH and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, tissue homogenates, saliva. Assay Type: Sandwich. Detection Range: 78 ng/ml -5000 ng/ml. Sensitivity: The minimum detectable dose of human STATH is typically less than 19.5 ng/ml.The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for STATH has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any STATH present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for STATH is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of STATH bound in the initial step. The color development is stopped and the intensity of the color is measured.

57164946

NP_001009181.1

NM_001009181.1

P02808

7,304 Da

Salivary Secretion Pathway (153376); Salivary Secretion Pathway (153352)

STATH: Salivary protein that stabilizes saliva supersaturated with calcium salts by inhibiting the precipitation of calcium phosphate salts. It also modulates hydroxyapatite crystal formation on the tooth surface. Belongs to the histatin/statherin family. Protein type: Secreted; Secreted, signal peptide; Extracellular matrix. Chromosomal Location of Human Ortholog: 4q13.3. Cellular Component: extracellular region. Molecular Function: protein binding; hydroxyapatite binding; structural constituent of tooth enamel; extracellular matrix constituent, lubricant activity. Biological Process: negative regulation of bone mineralization; ossification; biomineral formation; saliva secretion

48-Strip-Wells

510美元

96-Strip-Wells

725

5x96-Strip-Wells

2565

10x96-Strip-Wells

4800