ELISA/assay

Human Nuclease-sensitive element-binding protein 1, YBX1 ELISA Kit

MBS939527

48-Strip-Wells

510美元

酶联免疫吸附试剂盒

Human Nuclease-sensitive element-binding protein 1, YBX1 ELISA Kit

Y盒结合蛋白

Human Nuclease-sensitive element-binding protein 1 (YBX1) ELISA kit; BP-8; CSDA2; CSDB; DBPB; MDR-NF1; MGC104858; MGC110976; MGC117250; NSEP-1; NSEP1; YB-1; YB1; DNA-binding protein B; major histocompatibility complex; class II; Y box-binding protein I; nuclease sensit; Y box binding protein 1

nuclease-sensitive element-binding protein 1; Nuclease-sensitive element-binding protein 1; nuclease-sensitive element-binding protein 1; CBF-A; EFI-A; DNA-binding protein B; Y-box-binding protein 1; Y-box transcription factor; enhancer factor I subunit A; nuclease sensitive element binding protein 1; CCAAT-binding transcription factor I subunit A; major histocompatibility complex, class II, Y box-binding protein I; Y box binding protein 1; CCAAT-binding transcription factor I subunit A; CBF-A; DNA-binding protein B; DBPB; Enhancer factor I subunit A; EFI-A; Y-box transcription factor; Y-box-binding protein 1; YB-1

YBX1

YBX1;YBX1;YB1;碱基对-8;CSDB;DBPB;YB-1;CSDA2;NSEP1;NSEP-1;MDR-NF1;NSEP1;YB1;CBF-A;DBPB;EFI-A;YB-1

YBOX1_HUMAN

人类

324

This assay has high sensitivity and excellent specificity for detection of Human YBX1. No significant cross-reactivity or interference between Human YBX1 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, tissue homogenates, Cell lysates. Assay Type: Sandwich. Detection Range: 31.25 pg/ml-2000 pg/ml. Sensitivity: 7.81 pg/ml

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul. Protein Biological Process 1: Transcription/Transcription regulation. Protein Biological Process 3: Transcription

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for YBX1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any YBX1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for YBX1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of YBX1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

34098946

NP_004550.2

NM_004559.3

P67809

35,924 Da

Gene Expression Pathway (105937); IL-2 Signaling Pathway (198885); Processing Of Capped Intron-Containing Pre-mRNA Pathway (160950); SIDS Susceptibility Pathways (198901); Signaling Events Mediated By PTP1B Pathway (138017); MRNA Splicing Pathway (105951); MRNA Splicing - Major Pathway (105952); MRNA Processing Pathway (198843)

YB-1: a nuclear protein that binds to splice sites in pre-mRNA and regulates splice site selection. Binds and stabilizes cytoplasmic mRNA. Contributes to the regulation of translation by modulating the interaction between the mRNA and eukaryotic initiation factors CCAAT-containing Y-box of HLA class II genes. Component of cytoplasmic messenger ribonucleoprotein particles (mRNPs). Interacts with AKT1, SFRS9, THOC4, MSH2, XRCC5, WRN and NCL. Can bind to DNA as a homomeric form, (EFI-A)n or as a heteromeric form in association with EFI-B. Homodimer in the presence of ATP. Involved in cisplatin resistance. Seems to be a negative regulatory factor. May play a role in both transcriptional and translational regulation of spermatogenesis. Found at very low levels at day 10 and levels increase at day 15 and persist throughout adulthood. Protein type: RNA processing; Transcription factor; RNA splicing; RNA-binding. Chromosomal Location of Human Ortholog: 1p34. Cellular Component: nucleoplasm; nuclear membrane; intracellular membrane-bound organelle; stress granule; cytoplasm; ribonucleoprotein complex; nucleus; U12-dependent spliceosome. Molecular Function: protein binding; DNA binding; RNA binding; double-stranded DNA binding; transcription factor activity; single-stranded DNA binding. Biological Process: nuclear mRNA splicing, via spliceosome; negative regulation of striated muscle cell differentiation; transcription from RNA polymerase II promoter; regulation of transcription, DNA-dependent; in utero embryonic development; positive regulation of cell division; RNA splicing; gene expression; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription from RNA polymerase II promoter

48-Strip-Wells

510美元

96-Strip-Wells

725

5x96-Strip-Wells

2565

10x96-Strip-Wells

4800