ELISA/assay

Mouse Kelch-like ECH-associated protein 1, KEAP1 ELISA Kit

MBS924165

48-Strip-Wells

510美元

酶联免疫吸附试剂盒

Mouse Kelch-like ECH-associated protein 1, KEAP1 ELISA Kit

kelch-like ECH-associated protein 1

Mouse Kelch-like ECH-associated protein 1 (KEAP1) ELISA kit; INrf2; KIAA0132; KLHL19; MGC10630; MGC1114; MGC20887; MGC4407; MGC9454; cytosolic inhibitor of Nrf2; kelch-like ECH-associated protein 1

kelch-like ECH-associated protein 1; Kelch-like ECH-associated protein 1; kelch-like ECH-associated protein 1; ring canal protein; NRF2 cytosolic inhibitor; cytosolic inhibitor of Nrf2; kelch-like ECH-associated protein 1; Cytosolic inhibitor of Nrf2; INrf2

KEAP1

Keap1;Keap1;INRF2;mKIAA0132;Inrf2;Kiaa0132;INrf2

KEAP1_MOUSE

小鼠

624

This assay has high sensitivity and excellent specificity for detection of mouse KEAP1. No significant cross-reactivity or interference between mouse KEAP1 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, tissue homogenates, cell lysates. Assay Type: Sandwich. Detection Range: 4.7 pg/ml -300 pg/ml. Sensitivity: 1.17 pg /ml.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the Assay This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for KEAP1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any KEAP1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for KEAP1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of KEAP1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

160333659

NP_001103775.1

NM_001110305.1

Q9Z2X8

69,553 Da

Keap1-Nrf2 Pathway (198378); Ubiquitin Mediated Proteolysis Pathway (83253); Ubiquitin Mediated Proteolysis Pathway (466)

KEAP1: a substrate adapter protein for the E3 ubiquitin ligase complex formed by CUL3 and RBX1. Has tumor suppressor activity. Retains NRF2, BPTF and PGAM5 in the cytosol. Targets NRF2 for ubiquitination and degradation by the proteasome, thus resulting in the suppression of its transcriptional activity and of antioxidant response element-mediated detoxifying enzyme gene expression. Somatic mutations of KEAP1 or NRF2 commonly occur in solid cancers, irrespective of histological type, resulting in the constitutive transcription of cytoprotective genes. Genetic disruption of the KEAP1/CUL3 E3 ubiquitin ligase complex leads to the activation of the NF-kappaB pathway in lung cancer. Alternative activation of the cytoprotective NRF2 pathway exist in the absence of mutations in KEAP1 and HRF2: WTX, PALB2, SQSTM1, and DPP3 can competitively bind KEAP1. These proteins and others that bind KEAP1 contain an ¿ETGE¿ amino acid motif, which matches the KEAP1 interaction motif found in NRF2. Binding of these ¿ETGE¿-containing proteins can displace NRF2, freeing it to translocate to the nucleus and implement its cytoprotective transcriptional pathway. The DPP3 gene is frequently overexpressed in squamous cell lung carcinomas with high wt NRF2 activity. Its expression level significantly correlates with the response rate and progression-free survival of platinum-based first-line chemotherapy. May be a useful biomarker for predicting the chemosensitivity of patients with advanced-stage NSCLC. Broadly expressed, with highest levels in skeletal muscle. Ubiquitination and subsequent degradation of PGAM5 is inhibited by oxidative stress and sulforaphane. Protein type: Motility/polarity/chemotaxis; Ubiquitin conjugating system; Endoplasmic reticulum; Transcription regulation. Cellular Component: nucleoplasm; cell-cell adherens junction; focal adhesion; endoplasmic reticulum; cytoplasm; microtubule organizing center; midbody; nucleus. Molecular Function: protein binding; transcription factor binding. Biological Process: cytoplasmic sequestering of transcription factor; regulation of transcription, DNA-dependent; transcription, DNA-dependent; in utero embryonic development; proteasomal ubiquitin-independent protein catabolic process; protein ubiquitination; regulation of epidermal cell differentiation; protein oligomerization

48-Strip-Wells

510美元

96-Strip-Wells

725

5x96-Strip-Wells

2565

10x96-Strip-Wells

4800