产品简要
公司名称 :
MyBioSource
产品类型 :
抗体
产品名称 :
MAPE抗体(C末端)
目录 :
MBS9211992
规格 :
0.08毫升
价格 :
165美元
克隆性 :
多克隆
宿主 :
兔
共轭标签 :
未共轭
反应物种 :
人类
应用 :
免疫印迹, 酶联免疫吸附测定, 流式细胞仪, 酶免疫法, 免疫组化-石蜡切片
更多信息或购买 :
MyBioSource 产品网页
图像
图像 1 :
MyBioSource MBS9211992 图像 1
Overlay histogram showing Hela cells stained with AP6720b (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody MBS9211992, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(OH191631) at 1/200 dilution for 40 min at 37ºC. Isotypecontrol antibody (blue line) was rabbit IgG (1?g/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
图像 2 :
MyBioSource MBS9211992 图像 2
All lanes : Anti-MAPE Antibody (C-term) at 1:2000 dilution . Lane 1: Hela whole cell lysate . Lane 2: K562 whole cell lysate. Lane 3: 293 whole cell lysate. Lane 4: human testis lysate . Lane 5: human brain lysate . Lane 6: human kidney lysate Lysates/proteins at 20 ug perlane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 58 kDa . Blocking/Dilutionbuffer: 5% NFDM/TBST.
图像 3 :
MyBioSource MBS9211992 图像 3
MAPE Antibody (C-term) (RB18763) IHC analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the MAPE Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated
产品信息
目录号 :
MBS9211992
产品类型 :
抗体
产品全称 :
MAPE抗体(C末端)
产品简称 :
[MAPE]
产品名称同义词 :
[Melanoma antigen preferentially expressed in tumors; Opa-interacting protein 4; OIP-4; Preferentially expressed antigen of melanoma; PRAME; MAPE; OIP4]
其他名称 :
[melanoma antigen preferentially expressed in tumors isoform a; Melanoma antigen preferentially expressed in tumors; melanoma antigen preferentially expressed in tumors; preferentially expressed antigen in melanoma; Opa-interacting protein 4; OIP-4; Preferentially expressed antigen of melanoma]
产品基因名称 :
[PRAME]
其他基因名称 :
[PRAME;PRAME;MAPE;OIP4;CT130;OIP-4;MAPE;OIP4;OIP-4]
UniProt数据库进入名 :
PRAME_HUMAN
克隆性 :
多克隆
抗体亚型 :
兔IgE
宿主 :
兔
反应物种 :
人类
序列位置 :
[476-502]
序列长度 :
509
特异性 :
This MAPE antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 476-502 amino acids from the C-terminal region of human MAPE.
纯度 :
纯化兔多克隆抗体(多克隆抗体)
形式 :
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
储存稳定性 :
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
检测过的应用 :
流式细胞仪(FC), 免疫印迹(免疫印迹), 免疫组化P(免疫组化-P), 酶联免疫吸附测定(EIA)
应用笔记 :
FC~~ 1:25。 WB~~1:2000。 免疫组化-P~~1:50~100
image1头 :
试验数据(TD)
image2头 :
试验数据(TD)
image3头 :
试验数据(TD)
其他信息2 :
Function: Functions as a transcriptional repressor,inhibiting the signaling of retinoic acid through the retinoic acid receptors RARA, RARB and RARG. Prevents retinoic acid-induced cell proliferation arrest, differentiation and apoptosis. Cellular Location: Nucleus. Cell membrane . Tissue Location: Expressed in testis. Detected in samples of kidney, brain and skin
产品种类 :
Cancer
产品描述 :
MAPE is an antigen that is predominantly expressed in human melanomas and that is recognized by cytolytic T lymphocytes. It is not expressed in normal tissues, except testis. This expression pattern is similar to that of other CT antigens, such as MAGE, BAGE and GAGE. However, unlike these other CT antigens, its gene is also expressed in acute leukemias.
产品引用 :
Rezvani,K., Blood 113 (10), 2245-2255 (2009). Qin,Y., Leuk. Res. 33 (3), 384-390 (2009). Neumann,E., Cancer Res. 58 (18), 4090-4095 (1998)
NCBI GI登录号 :
5174641
NCBI登录号 :
NP_006106.1
NCBI基因登录号 :
NM_006115.4
UniProt数据库登录号 :
P78395
NCBI总结 :
This gene encodes an antigen that is preferentially expressed in human melanomas and that is recognized by cytolytic T lymphocytes. It is not expressed in normal tissues, except testis. The encoded protein acts as a repressor of retinoic acid receptor, and likely confers a growth advantage to cancer cells via this function. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2014]
UniProt数据库总结 :
PRAME: Functions as a transcriptional repressor, inhibiting the signaling of retinoic acid through the retinoic acid receptors RARA, RARB and RARG. Prevents retinoic acid-induced cell proliferation arrest, differentiation and apoptosis. Belongs to the PRAME family. Protein type: Transcription, coactivator/corepressor; Cancer Testis Antigen (CTA); Nuclear receptor co-regulator. Chromosomal Location of Human Ortholog: 22q11.22. Cellular Component: plasma membrane; nucleus. Molecular Function: protein binding; retinoic acid receptor binding. Biological Process: negative regulation of retinoic acid receptor signaling pathway; negative regulation of cell differentiation; transcription, DNA-dependent; apoptosis; regulation of growth; positive regulation of cell proliferation; cell differentiation; negative regulation of transcription, DNA-dependent; negative regulation of apoptosis
尺寸1 :
0.08毫升
价格1 :
165美元
尺寸2 :
0.4毫升
价格2 :
370
更多信息或购买 :
MyBioSource 产品网页
公司信息
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com
1-858-633-0165
公司总部: 美国
MyBioSource,LLC最初由三名热情澎湃的提供高品质试剂的科学家联合创立于温哥华，公司愿景是“生物研究试剂的源头”，现在位于圣地亚哥市。