抗体

EN1 (Engrailed 1) Antibody (N-term)

MBS9211211

0.08毫升

165美元

多克隆

兔

未共轭

免疫印迹, 酶联免疫吸附测定, 免疫组化, 免疫细胞化学, 酶免疫法

抗体

EN1 (Engrailed 1) Antibody (N-term)

[EN1 (Engrailed 1)]

[Homeobox protein engrailed-1; Homeobox protein en-1; Hu-En-1; EN1]

[homeobox protein engrailed-1; Homeobox protein engrailed-1; homeobox protein engrailed-1; engrailed homeobox 1]

[EN1]

[EN1; EN1; Homeobox protein en-1; Hu-En-1]

HME1_HUMAN

多克隆

兔IgE

兔

人类

[1-30]

392

This EN1 (Engrailed 1) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human EN1 (Engrailed 1).

肽亲和纯化兔多克隆抗体(多克隆抗体)

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Vial Concentration: 0.5

Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

免疫印迹(免疫印迹), 酶联免疫吸附测定(EIA), 免疫组化(免疫组化), 免疫荧光(IF)

WB~~1:1000。 IF~~1:10~50。 免疫组化-P~~1:10~50

免疫印迹(免疫印迹)

免疫印迹

免疫印迹

Immunofluorescent

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0. 1% Triton X-100 permeabilized U-2 OS (Human Sarcoma cell line) cells labeling Pdx1 with MBS9211211 at 1/25 dilution, followed by Alexa Fluor 488-conjugated goat anti-rabbit IgG (1583138) secondary antibody at 1/400 dilution (green). Immunofluorescence image showing nucleus and nucleoli staining on U-2 OS cell line. Cytoplasmic actin is detected with Alexa Fluor® 555 conjugated with Phalloidin (OB16636430) at 1/100 dilution (red).

免疫组化

MBS9211211 staining EN1 in Human tonsil tissue sections by Immunohistochemistry (IHC-P-paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.

Antigen Type: Synthetic Peptide. Crown Antibody: Yes

Antigen Source: HUMAN. Clone Names: RB13885

牙冠抗体;发育生物学;神经科学;信号转导

Homeobox-containing genes are thought to have a role in controlling development. In Drosophila, the 'engrailed' (en) gene plays an important role during development in segmentation, where it is required for the formation of posterior compartments. Different mutations in the mouse homologs, En1 and En2, produced different developmental defects that frequently are lethal. The human engrailed homologs 1 and 2 encode homeodomain-containing proteins and have been implicated in the control of pattern formation during development of the central nervous system.

Bachar-Dahan,L., Mol. Biol. Cell 17 (6), 2572-2580 (2006). Kohler,A., Genomics 15 (1), 233-235 (1993)

126090909

NP_001417.3

NM_001426.3

Q05925

40115

Homeobox-containing genes are thought to have a role in controlling development. In Drosophila, the 'engrailed' (en) gene plays an important role during development in segmentation, where it is required for the formation of posterior compartments. Different mutations in the mouse homologs, En1 and En2, produced different developmental defects that frequently are lethal. The human engrailed homologs 1 and 2 encode homeodomain-containing proteins and have been implicated in the control of pattern formation during development of the central nervous system. [provided by RefSeq, Jul 2008]

EN1: Homeobox-containing genes are thought to have a role in controlling development. In Drosophila, the engrailed (en) gene plays an important role during development in segmentation, where it is required for the formation of posterior compartments. Different mutations in the mouse homologs, En1 and En2, produced different developmental defects that frequently are lethal. The human engrailed homologs 1 and 2 encode homeodomain-containing proteins and have been implicated in the control of pattern formation during development of the central nervous system. [provided by RefSeq, Jul 2008]. Protein type: Cell development/differentiation; DNA-binding; Transcription factor. Chromosomal Location of Human Ortholog: 2q14.2. Cellular Component: membrane; nucleus. Biological Process: embryonic forelimb morphogenesis; anatomical structure morphogenesis; dorsal/ventral pattern formation; pigmentation; multicellular organism growth; midbrain development; midbrain-hindbrain boundary development; neuron development; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription from RNA polymerase II promoter; response to cocaine; skeletal development; proximal/distal pattern formation; hindbrain development

0.08毫升

165美元

0.4毫升

370