抗体

CD133抗体

MBS857565

0.1毫升

290美元

多克隆

兔

未共轭

人类, 小鼠, 大鼠

免疫印迹, 免疫组化, 免疫细胞化学

抗体

CD133抗体

CD133

prominin-1 isoform 2; Prominin-1; prominin-1; hProminin; antigen AC133; prominin-like protein 1; hematopoietic stem cell antigen; prominin 1; Antigen AC133; Prominin-like protein 1; CD_antigen: CD133

CD133

PROM1;PROM1;RP41;AC133;CD133;MCDR2;STGD4;CORD12;PROML1;MSTP061;PROML1

PROM1_HUMAN

多克隆

IgG

兔

Human, Mouse, Rat

856

This antibody is produced by immunizing rabbits with a synthetic peptide (KLH-coupled) corresponding to a region of C-terminal residues between 800-900aa of human and mouse CD133.

亲和纯化

液体

100微升, 0.74毫克/毫升

Store at 4 degree C after thawing. Aliquot store at -20 degree C or -80 degree C. Avoid repeated freeze / thaw cycles.

Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)

WB: 1:1, 000, ICC: 1:200, IHC: 1:200

Positive Control: Mouse Kidney tissue

Cellular Localization: Cell membrane. Storage Buffer: 1*TBS (pH7.4), 0.5%BSA, 25%Glycerol. Preservative: 0.05% Sodium Azide.

Cancer; Cardiovascular; Cell Biology; Epigenetics & Nuclear Signaling; Developmental Biologys; Immunology; Drug Discovery Products; Metabolism; Neuroscience; Signal Transduction; Stem Cells; Autophagy antibody

Background: CD133 is a glycoprotein also known in humans and rodents as Prominin 1 (PROM1). It is the founding member of pentaspan transmembrane glycoproteins (5-transmembrane, 5-TM), which specifically localizes to cellular protrusions. CD133 is expressed in hematopoietic stem cells, endothelial progenitor cells, glioblastomas, neuronal and glial stem cells and some other cell types. CD133 is recognized as a stem cell marker for normal and cancerous tissues.

1. Yin AH, Miraglia S, Zanjani ED,et al. CD133, a novel marker for human hematopoietic stem and progenitor cells. Blood,1997; 90:5002-5012. 2. Wuchter C,Ratei R,Spahn G,et al. Impact of CD133 (CD133) and CD90 expression analysis for acute leukemia immunophenotyping. Haematologica, 2001; 86:154-161. 3. Kuci S,Schumn M,Schlegel PG,et al. Phenotypic and functional characterization of mobilized peripheral blood CD133+CD34+ hematopoietic stem cell. Blood,1999; 94(Suppl 1):559a.

224994189

NP_001139319.1

NM_001145847.1

O43490

~120kDa

Transcriptional Misregulation In Cancer Pathway (523016); Transcriptional Misregulation In Cancer Pathway (522987)

This gene encodes a pentaspan transmembrane glycoprotein. The protein localizes to membrane protrusions and is often expressed on adult stem cells, where it is thought to function in maintaining stem cell properties by suppressing differentiation. Mutations in this gene have been shown to result in retinitis pigmentosa and Stargardt disease. Expression of this gene is also associated with several types of cancer. This gene is expressed from at least five alternative promoters that are expressed in a tissue-dependent manner. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009]

CD133: Binds cholesterol in cholesterol-containing plasma membrane microdomains. Proposed to play a role in apical plasma membrane organization of epithelial cells. During early retinal development acts as a key regulator of disk morphogenesis. Involved in regulation of MAPK and Akt signaling pathways. In neuroblastoma cells suppresses cell differentiation such as neurite outgrowth in a RET-dependent manner. Interacts with CDHR1 and with actin filaments. Isoform 1 is selectively expressed on CD34 hematopoietic stem and progenitor cells in adult and fetal bone marrow, fetal liver, cord blood and adult peripheral blood. Isoform 1 is not detected on other blood cells. Isoform 1 is also expressed in a number of non-lymphoid tissues including retina, pancreas, placenta, kidney, liver, lung, brain and heart. Found in saliva within small membrane particles. Isoform 2 is predominantly expressed in fetal liver, skeletal muscle, kidney, and heart as well as adult pancreas, kidney, liver, lung, and placenta. Isoform 2 is highly expressed in fetal liver, low in bone marrow, and barely detectable in peripheral blood. Isoform 2 is expressed on hematopoietic stem cells and in epidermal basal cells. Expressed in adult retina by rod and cone photoreceptor cells. Belongs to the prominin family. 7 isoforms of the human protein are produced by alternative splicing. Protein type: Cell surface; Membrane protein, multi-pass; Membrane protein, integral. Chromosomal Location of Human Ortholog: 4p15.32. Cellular Component: extracellular space; stereocilium; photoreceptor outer segment; cell surface; integral to plasma membrane; endoplasmic reticulum; microvillus membrane; apical plasma membrane; plasma membrane; ER-Golgi intermediate compartment; vesicle; brush border. Molecular Function: actinin binding; protein binding; cadherin binding. Biological Process: photoreceptor cell maintenance; retina morphogenesis in camera-type eye. Disease: Stargardt Disease 4; Macular Dystrophy, Retinal, 2; Retinitis Pigmentosa 41; Cone-rod Dystrophy 12

0.1毫升

290美元