抗体

HSF1(磷酸化Ser307)抗体

MBS857430

0.1毫克

215美元

多克隆

兔

未共轭

免疫印迹, 免疫细胞化学

抗体

HSF1(磷酸化Ser307)抗体

HSF1

heat shock factor protein 1; Heat shock factor protein 1; heat shock factor protein 1; HSF 1; HSTF 1; heat shock transcription factor 1; Heat shock transcription factor 1; HSTF 1

HSF1

HSF1;HSF1;HSTF1;HSTF1;HSF 1;HSTF 1

HSF1_HUMAN

多克隆

IgG

兔

人类

529

HSF1 (Phospho-Ser307) Antibody detects endogenous levels of HSF1 only when phosphorylated at serine 307.

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

50微克/50微升

Store at -20 degree C for 1 year

免疫印迹(免疫印迹), 免疫荧光(IF)

WB: 1:500~1:1000, IF: 1:100-200

Immunogen: The antiserum was produced against synthesized phosphopeptide derived from human HSF1 around the phosphorylation site of serine 307 (P-Q-SP-P-R). Pathway: MAPK, Chromatin/Transcription

Cancer; Cardiovascular; Cell Biology; Epigenetics & Nuclear Signaling; Developmental Biologys; Immunology; Drug Discovery Products; Metabolism; Neuroscience; Signal Transduction; Stem Cells; Autophagy antibody

Guettouche T, et al. (2005) BMC Biochem ; 6(1): 4. Wang X, et al. (2003) Mol Cell Biol ; 23(17): 6013-6026. Jolly C, et al. (1999) Proc Natl Acad Sci USA; 96(12): 6769-6774.

5031767

NP_005517.1

NM_005526.2

Q00613

52,881 Da

Attenuation Phase Pathway (980473); Cellular Response To Heat Stress Pathway (980470); Cellular Responses To Stress Pathway (645258); HSF1 Activation Pathway (980471); HSF1-dependent Transactivation Pathway (980472); Legionellosis Pathway (469200); Legionellosis Pathway (469186); Regulation Of HSF1-mediated Heat Shock Response Pathway (980474)

The product of this gene is a heat-shock transcription factor. Transcription of heat-shock genes is rapidly induced after temperature stress. Hsp90, by itself and/or associated with multichaperone complexes, is a major repressor of this gene. [provided by RefSeq, Jul 2008]

HSF1: a transcription factor that specifically binds heat shock promoter elements (HSE) and activates transcription. Induced in response to heat, heavy metals, and oxidative stress. In higher eukaryotes, HSF is unable to bind to HSEs unless the cells are stressed. Becomes phosphorylated in response to stress, forming homotrimers that bind DNA and activate transcription. Phosphorylation by PLK1 enhances nuclear translocation, and phosphorylation by CaMKII enhances transactivation. Phosphorylation by GSK3 and ERK1 induces binding by 14-3-3 and sequestration in the cytoplasm. In addition, during attenuation from the heat shock response, HSF1 is repressed by direct binding of Hsp70, HSP40, and HSF binding protein 1 (HSBP1). Four alternatively spliced isoforms have been described. Protein type: DNA-binding; Transcription factor. Chromosomal Location of Human Ortholog: 8q24.3. Cellular Component: nucleoplasm; protein complex; pronucleus; cytoplasm; cytosol. Molecular Function: protein binding; chromatin binding; transcription factor activity. Biological Process: embryonic placenta development; embryonic process involved in female pregnancy; female meiosis; defense response; response to lipopolysaccharide; negative regulation of transcription from RNA polymerase II promoter; negative regulation of tumor necrosis factor production; positive regulation of multicellular organism growth; protein amino acid phosphorylation; negative regulation of cell proliferation; mRNA transcription; positive regulation of transcription from RNA polymerase II promoter; spermatogenesis

0.1毫克

215美元

0.05毫克

225