抗体

细胞周期蛋白D2(磷酸化Thr280)抗体

MBS854617

0.1毫克

215美元

多克隆

兔

未共轭

人类, 小鼠, 大鼠

免疫印迹, 酶联免疫吸附测定, 酶免疫法

抗体

细胞周期蛋白D2(磷酸化Thr280)抗体

细胞周期蛋白D2

cyclin D2; G1/S-specific cyclin D2

G1/S-specific cyclin-D2; G1/S-specific cyclin-D2; G1/S-specific cyclin-D2; cyclin D2

CCND2;CCND2;MPPH3;KIAK0002

CCND2_HUMAN

兔

人类, 小鼠, 大鼠

289

Cyclin D2 (Phospho-Thr280) antibody detects endogenous levels of Cyclin D2 only when phosphorylated at threonine 280.

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody againstnon-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation s

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Store at -20 degree C for 1 year

免疫印迹(免疫印迹), 酶联免疫吸附测定(EIA)

WB: 1:500~1:1000, ELISA: 1:1000

Reactivity Note: Human (Identities = 100%, Positives = 100%); Mouse (Identities = 90%, Positives = 100%); Rat (Identities = 90%, Positives = 100%)

Immunogen: The antiserum was produced against synthesized phosphopeptide derived from human Cyclin D2 around the phosphorylation site of threonine 280 (A-S-TP-P-T). All Sites: Human: Thr280; Mouse: Thr279; Rat: Thr279. Pathway: Phospho-specific

Cancer; Cardiovascular; Cell Biology; Epigenetics & Nuclear Signaling; Developmental Biologys; Immunology; Drug Discovery Products; Metabolism; Neuroscience; Signal Transduction; Stem Cells; Autophagy antibody

Xiong Y., Genomics 13:575-584(1992). Palmero I., Oncogene 8:1049-1054(1993). Kalnine N., Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases.

4502617

NP_001750.1

NM_001759.3

P30279

33,067 Da

B Cell Receptor Signaling Pathway (198909); Cell Cycle Pathway (530733); Cell Cycle, Mitotic Pathway (105765); Cell Cycle Pathway (198811); Cell Cycle Pathway (83054); Cell Cycle Pathway (463); Cyclin D Associated Events In G1 Pathway (105767); DNA Damage Response Pathway (198788); DNA Damage Response (only ATM Dependent) Pathway (198827); Focal Adhesion Pathway (198795)

The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with CDK4 or CDK6 and functions as a regulatory subunit of the complex, whose activity is required for cell cycle G1/S transition. This protein has been shown to interact with and be involved in the phosphorylation of tumor suppressor protein Rb. Knockout studies of the homologous gene in mouse suggest the essential roles of this gene in ovarian granulosa and germ cell proliferation. High level expression of this gene was observed in ovarian and testicular tumors. Mutations in this gene are associated with megalencephaly-polymicrogyria-polydactyly-hydrocephalus syndrome 3 (MPPH3). [provided by RefSeq, Sep 2014]

CCND2: Regulatory component of the cyclin D2-CDK4 (DC) complex that phosphorylates and inhibits members of the retinoblastoma (RB) protein family including RB1 and regulates the cell-cycle during G(1)/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complex and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase. Hypophosphorylates RB1 in early G(1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also substrate for SMAD3, phosphorylating SMAD3 in a cell-cycle-dependent manner and repressing its transcriptional activity. Component of the ternary complex, cyclin D2/CDK4/p27Kip1, required for nuclear translocation and activity of the cyclin D-CDK4 complex. Interacts with either CDK4 or CDK6 protein kinase to form a serine/threonine kinase holoenzyme complex. The cyclin subunit imparts substrate specificity to the complex. Component of the ternary complex cyclin D/CDK4/p27Kip1 required for nuclear translocation and modulation of CDK4-mediated kinase activity. Belongs to the cyclin family. Cyclin D subfamily. Protein type: Motility/polarity/chemotaxis; Cell cycle regulation. Chromosomal Location of Human Ortholog: 12p13. Cellular Component: nucleoplasm; nuclear membrane; nucleolus; cyclin-dependent protein kinase holoenzyme complex; chromatin; nucleus; cytosol. Molecular Function: protein binding; protein kinase binding. Biological Process: positive regulation of cyclin-dependent protein kinase activity; cell division; positive regulation of protein amino acid phosphorylation; cell cycle. Disease: Megalencephaly-polymicrogyria-polydactyly-hydrocephalus Syndrome 3

0.1毫克

215美元