POLR2A(Ab-1619)抗体 | MyBioSource MBS8502228 产品信息

产品简要

公司名称 :

MyBioSource

产品类型 :

抗体

产品名称 :

POLR2A(Ab-1619)抗体

MBS8502228

规格 :

0.1毫克

价格 :

280美元

克隆性 :

多克隆

宿主 :

兔

共轭标签 :

未共轭

反应物种 :

人类, 小鼠, 大鼠

应用 :

免疫印迹, 酶联免疫吸附测定, 免疫组化, 免疫细胞化学, 酶免疫法

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图像

图像 1 :

Peptide: - / + : Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using POLR2A (Ab-1619) antibody.

图像 2 :

Western blot analysis of extracts from COS7 cells treated with EGF (200ng/ml, 30min), using POLR2A (Ab-1619) antibody.

图像 3 :

PMA: + / - : Immunofluorescence analysis of HeLa cells, treated with PMA (125ng/ml, 30mins), using POLR2A (Ab-1619) antibody.

产品信息

目录号 :

MBS8502228

产品类型 :

抗体

产品全称 :

POLR2A(Ab-1619)抗体

产品简称 :

[POLR2A]

产品名称同义词 :

[RPO2; RPOL2; POLR2 ALPHA-AMANITIN RESISTANCE; POLYMERASE II RNA; SUBUNIT A; RNA POLYMERASE II; 220-KD SUBUNIT; RNA POLYMERASE II; LARGE SUBUNIT; RPB1; S. CEREVISIAE; HOMOLOG OF polymerase (RNA) II (DNA directed) polypeptide A]

其他名称 :

[DNA-directed RNA polymerase II subunit RPB1; DNA-directed RNA polymerase II subunit RPB1; DNA-directed RNA polymerase II subunit RPB1; RNA polymerase II subunit A; DNA-directed RNA polymerase II subunit A; DNA-directed RNA polymerase III largest subunit; RNA-directed RNA polymerase II subunit RPB1 (EC:2.7.7.48)]

产品基因名称 :

[POLR2A]

其他基因名称 :

[POLR2A;POLR2A;RPB1;RPO2;POLR2;POLRA;RPBh1;RPOL2;RpIILS;hsRPB1;hRPB220;POLR2;RNA聚合酶II亚单位B1]

克隆性 :

多克隆

抗体亚型 :

IgG

宿主 :

兔

反应物种 :

人类, 小鼠, 大鼠

序列长度 :

1970

特异性 :

POLR2A(Ab-1619)抗体检测内生水平总数POLR2A蛋白

纯度 :

Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

形式 :

In phosphate buffered saline (without Mg 2+ and Ca 2+ ), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

储存稳定性 :

Store at-20 degree C for 1 year

检测过的应用 :

免疫印迹(免疫印迹), 免疫组化(免疫组化), 免疫荧光(IF), 酶联免疫吸附测定(EIA)

应用笔记 :

WB: 1:500~1:1000。 IHC: 1:50~1:100。 IF: 1:100~1:500。 ELISA: 1:10000

image1头 :

免疫组化(免疫组化)

image2头 :

免疫印迹(免疫印迹)

image3头 :

免疫荧光(IF)

其它信息1 :

Immunogen: The antiserum was produced against synthesized non-phosphopeptide derived from human POLR2A around the phosphorylation site of serine 1619 (P-T-S-P-S).

产品种类 :

Autophagy antibody; Cancer; Cardiovascular; Cell Biology; Epigenetics & Nuclear Signaling; Developmental Biologys; Immunology; Drug Discovery Products; Metabolism; Neuroscience; Signal Transduction; Stem Cells

产品引用 :

Tracy Jo Pasieka, J. Virol., Aug 2006; 80: 7600-7612. Olaf R. Mook, Mol. Cancer Ther., Mar 2007; 6: 833-843. Colleen A. McMullen, J. Neurosci., Jan 2004; 24: 161.

NCBI GI登录号 :

4505939

NCBI登录号 :

NP_000928.1

NCBI基因登录号 :

NM_000937.4

UniProt数据库登录号 :

P24928

NCBI信号通路 :

Abortive Elongation Of HIV-1 Transcript In The Absence Of Tat Pathway (106005); DNA Repair Pathway (105837); Disease Pathway (530764); Dual Incision Reaction In TC-NER Pathway (105892); Epstein-Barr Virus Infection Pathway (585562); Epstein-Barr Virus Infection Pathway (587115); Eukaryotic Transcription Initiation Pathway (198917); Formation Of HIV-1 Elongation Complex Containing HIV-1 Tat Pathway (106004); Formation Of HIV-1 Elongation Complex In The Absence Of HIV-1 Tat Pathway (161052); Formation Of RNA Pol II Elongation Complex Pathway (105950)

NCBI总结 :

This gene encodes the largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. The product of this gene contains a carboxy terminal domain composed of heptapeptide repeats that are essential for polymerase activity. These repeats contain serine and threonine residues that are phosphorylated in actively transcribing RNA polymerase. In addition, this subunit, in combination with several other polymerase subunits, forms the DNA binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA. [provided by RefSeq, Jul 2008]

UniProt数据库总结 :

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Largest and catalytic component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Forms the polymerase active center together with the second largest subunit. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB1 is part of the core element with the central large cleft, the clamp element that moves to open and close the cleft and the jaws that are thought to grab the incoming DNA template. At the start of transcription, a single-stranded DNA template strand of the promoter is positioned within the central active site cleft of Pol II. A bridging helix emanates from RPB1 and crosses the cleft near the catalytic site and is thought to promote translocation of Pol II by acting as a ratchet that moves the RNA-DNA hybrid through the active site by switching from straight to bent conformations at each step of nucleotide addition. During transcription elongation, Pol II moves on the template as the transcript elongates. Elongation is influenced by the phosphorylation status of the C-terminal domain (CTD) of Pol II largest subunit (RPB1), which serves as a platform for assembly of factors that regulate transcription initiation, elongation, termination and mRNA processing. Regulation of gene expression levels depends on the balance between methylation and acetylation levels of tha CTD-lysines (). Initiation or early elongation steps of transcription of growth-factors-induced immediate early genes are regulated by the acetylation status of the CTD (PubMed:24207025). Methylation and dimethylation have a repressive effect on target genes expression ().

尺寸1 :

0.1毫克

价格1 :

280美元

更多信息或购买 :

MyBioSource 产品网页

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