ELISA/assay

Human Soluble receptor activator of nuclear factor kB ligand ELISA Kit

MBS724855

48-Strip-Wells

470美元

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Human Soluble receptor activator of nuclear factor kB ligand ELISA Kit

Soluble receptor activator of nuclear factor kB ligand

SRANKL

人类

This assay has high sensitivity and excellent specificity for detection of sRANKL. No significant cross-reactivity or interference between sRANKL and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between sRANKL and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Sandwich. Sensitivity: 1.0 pg/mL.

Intended Uses: This sRANKL ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human sRANKL. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

信号转导

. Principle of the assay: sRANKL ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for sRANKL. Standards or samples are then added to the microtiter plate wells and sRANKL if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of sRANKL present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for sRANKL are added to each well to "sandwich" the sRANKL immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain sRANKL and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The sRANKL concentration in each sample is interpolated from this standard curve.

48-Strip-Wells

470美元

96-Strip-Wells

675