产品简要
公司名称 :
MyBioSource
产品类型 :
ELISA/assay
产品名称 :
Monkey Vascular Endothelial cell Growth Factor, VEGF ELISA Kit
目录 :
MBS706135
规格 :
48-Strip-Wells
价格 :
565美元
更多信息或购买 :
产品信息
目录号 :
MBS706135
产品类型 :
酶联免疫吸附试剂盒
产品全称 :
Monkey Vascular Endothelial cell Growth Factor, VEGF ELISA Kit
产品简称 :
血管内皮细胞生长因子(血管内皮生长因子)
产品名称同义词 :
Monkey Vascular Endothelial cell Growth Factor (VEGF) ELISA Kit; Vascular Endothelial cell Growth Factor (VEGF)
其他名称 :
血管内皮生长因子;血管内皮生长因子;血管内皮生长因子;血管通透性因子;血管内皮生长因子;血管通透性因子;VPF
产品基因名称 :
血管内皮生长因子
其他基因名称 :
血管内皮生长因子A;血管内皮生长因子A;VPF;血管内皮生长因子;MVCD1;血管内皮生长因子;血管内皮生长因子-A;VPF
UniProt数据库进入名 :
VEGFA_HUMAN
反应物种 :
Monkey
序列长度 :
191
特异性 :
This assay has high sensitivity and excellent specificity for detection of monkey VEGF. No significant cross-reactivity or interference between monkey VEGF and analogues was observed.
储存稳定性 :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
其它信息1 :
Samples: Serum, plasma, cell culture supernates, Cell lysates. Assay Type: Sandwich. Detection Range: 125 pg/ml -8000 pg/ml. Sensitivity: The minimum detectable dose of monkey VEGF is typically less than 31.25 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
其他信息2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
产品描述 :
Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for VEGF has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any VEGF present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for VEGF is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of VEGF bound in the initial step. The color development is stopped and the intensity of the color is measured.
NCBI GI登录号 :
181971
NCBI登录号 :
AAA35789.1
UniProt数据库登录号 :
P15692
NCBI分子量 :
34,406 Da
NCBI信号通路 :
Angiogenesis Pathway (198772); Axon Guidance Pathway (105688); Bladder Cancer Pathway (83115); Bladder Cancer Pathway (527); Cellular Response To Hypoxia Pathway (645259); Cellular Responses To Stress Pathway (645258); Cytokine-cytokine Receptor Interaction Pathway (83051); Cytokine-cytokine Receptor Interaction Pathway (460); Developmental Biology Pathway (477129); EPH-Ephrin Signaling Pathway (1016174)
NCBI总结 :
This gene is a member of the PDGF/VEGF growth factor family and encodes a protein that is often found as a disulfide linked homodimer. This protein is a glycosylated mitogen that specifically acts on endothelial cells and has various effects, including mediating increased vascular permeability, inducing angiogenesis, vasculogenesis and endothelial cell growth, promoting cell migration, and inhibiting apoptosis. Elevated levels of this protein is linked to POEMS syndrome, also known as Crow-Fukase syndrome. Mutations in this gene have been associated with proliferative and nonproliferative diabetic retinopathy. Alternatively spliced transcript variants, encoding either freely secreted or cell-associated isoforms, have been characterized. There is also evidence for the use of non-AUG (CUG) translation initiation sites upstream of, and in-frame with the first AUG, leading to additional isoforms. [provided by RefSeq, Jul 2008]
UniProt数据库总结 :
Function: Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. NRP1/Neuropilin-1 binds isoforms VEGF-165 and VEGF-145. Isoform VEGF165B binds to KDR but does not activate downstream signaling pathways, does not activate angiogenesis and inhibits tumor growth.3 PublicationsManual assertion based on experiment in:Ref.27
尺寸1 :
48-Strip-Wells
价格1 :
565美元
尺寸2 :
96-Strip-Wells
价格2 :
810
size3 :
5x96-Strip-Wells
价格3 :
2750
size4 :
10x96-Strip-Wells
price4 :
5150
更多信息或购买 :
公司信息
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MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com1-858-633-0165
公司总部: 美国
MyBioSource,LLC最初由三名热情澎湃的提供高品质试剂的科学家联合创立于温哥华,公司愿景是“生物研究试剂的源头”,现在位于圣地亚哥市。
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