抗体

胰岛素受体, 乙(内部评级)

MBS632810

0.5毫升

725美元

单克隆

小鼠

未共轭

[3F225]

人类, 小鼠, 大鼠

免疫印迹, 酶联免疫吸附测定, 免疫组化

抗体

胰岛素受体, 乙(内部评级)

[胰岛素受体, 乙]

[Anti -Insulin Receptor, beta (IRb)]

[insulin receptor isoform Long preproprotein; Insulin receptor; insulin receptor; IR; insulin receptor]

[INSR;INSR;HHF5;CD220;IR]

INSR_HUMAN

单克隆

IgG1

[3F225]

小鼠

monkey, mouse and rat.

识别人类胰岛素受体, 乙(95kD)

Purified by Protein G affinity chromatography from ascites.

Supplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide, before the addition of glycerol to 40%. Also available without BSA and azide at 1mg/ml. See MBS632969.

0.2毫克/毫升

May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Affinity Purification, Conjugation, ELISA, Western Blot, Tyrosine Kinase Assay, Immunohistochemistry.

Suitable for use in Affinity Purification, Conjugation, ELISA, Western Blot, Tyrosine Kinase Assay, Immunohistochemistry. Not suitable for use in Immunoprecipitation. Dilution: Affinity Purification: With peptide elution. Conjugation: Use MBS632969. ELISA (Capture Ab): Use MBS632969. Tyrosine Kinase Assay: Ab-mediated capture on microtitre plates. Western Blot: 1-2ug/ml for 2hrs at RT. Immunohistochemistry (Formalin/paraffin): 1:50 for 30 min at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 1mM EDTA, pH 8.0, for 10-20 min followed by cooling at RT for 20 min. Optimal dilution to be determined by researcher.

免疫组化(免疫组化)

免疫组化(免疫组化)

免疫组化(免疫组化)

Grade: Affinity Purified. Immunogen: Recombinant fragment including the C-terminal 100 amino acids of human insulin receptor. Cellular Localization: Cell membrane

Positive Control: HEP-G-2, IM-9 lymphocyte cells, placenta or brain.

Antibodies; Abs to Receptors

The human insulin receptor is a heterotetrameric membrane glycoprotein consisting of disulfide-linked subunits in a b-a-a-b configuration. The b-subunit (95kD) possesses a single transmembrane domain with tyrosine kinase acivity, whereas the a-subunit (135kD) is completely extracellular.

119395736

NP_000199.2

NM_000208.2

P06213

156,333 Da

AGE/RAGE Pathway (698754); AMPK Signaling Pathway (198868); Adherens Junction Pathway (83070); Adherens Junction Pathway (481); Aldosterone-regulated Sodium Reabsorption Pathway (130626); Aldosterone-regulated Sodium Reabsorption Pathway (130590); DNA Damage Response (only ATM Dependent) Pathway (198827); HIF-1 Signaling Pathway (695200); IRS Activation Pathway (106425); IRS-related Events Pathway (106424)

After removal of the precursor signal peptide, the insulin receptor precursor is post-translationally cleaved into two chains (alpha and beta) that are covalently linked. Binding of insulin to the insulin receptor (INSR) stimulates glucose uptake. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

INSR: a receptor tyrosine kinase that mediates the pleiotropic actions of insulin. Binding of insulin leads to phosphorylation of several intracellular substrates, including, insulin receptor substrates (IRS1, 2, 3, 4), SHC, GAB1, CBL and other signaling intermediates. Each of these phosphorylated proteins serve as docking proteins for other signaling proteins that contain Src-homology-2 domains (SH2 domain) that specifically recognize different phosphotyrosines residues, including the p85 regulatory subunit of PI3K and SHP2. Phosphorylation of IRSs proteins lead to the activation of two main signaling pathways: the PI3K-AKT pathway, which is responsible for most of the metabolic actions of insulin, and the Ras-MAPK pathway, which regulates expression of some genes and cooperates with the PI3K pathway to control cell growth and differentiation. In addition to binding insulin, the insulin receptor can bind insulin-like growth factors (IGFI and IGFII). The holoenzyme is cleaved into two chains, the alpha and beta subunits. The active complex is a tetramer containing 2 alpha and 2 beta chains linked by disulfide bonds. The alpha chains constitute the ligand- binding domain, while the beta chains carry the kinase domain. Interacts with SORBS1 but dissociates from it following insulin stimulation. Familial mutations associated with insulin resistant diabetes, acanthosis nigricans, pineal hyperplasia, and polycystic ovary syndrome. SNP variants may be associated with polycystic ovary syndrome, atypical migraine and diabetic hyperlipidemia. Mutations also cause leprechaunism, a severe insulin resistance syndrome causing growth retardation and death in early infancy. Two isoforms of the human protein are produced by alternative splicing. The Short isoform has a higher affinity for insulin than the longer. Isoform Long and isoform Short are predominantly expressed in tissue targets of insulin metabolic effects: liver, adipose tissue and skeletal muscle but are also expressed in the peripheral nerve, kidney, pulmonary alveoli, pancreatic acini, placenta vascular endothelium, fibroblasts, monocytes, granulocytes, erythrocytes and skin. Isoform Short is preferentially expressed in fetal cells such as fetal fibroblasts, muscle, liver and kidney. Found as a hybrid receptor with IGF1R in muscle, heart, kidney, adipose tissue, skeletal muscle, hepatoma, fibroblasts, spleen and placenta. Overexpressed in several tumors, including breast, colon, lung, ovary, and thyroid carcinomas. Protein type: Kinase, protein; Membrane protein, integral; Protein kinase, TK; EC 2.7.10.1; Protein kinase, tyrosine (receptor); TK group; InsR family. Chromosomal Location of Human Ortholog: 19p13.3-p13.2. Cellular Component: intracellular membrane-bound organelle; membrane; integral to plasma membrane; plasma membrane; endosome membrane; caveola; receptor complex. Molecular Function: insulin binding; insulin-like growth factor receptor binding; protein binding; insulin-like growth factor I binding; GTP binding; insulin receptor substrate binding; protein-tyrosine kinase activity; PTB domain binding; phosphoinositide 3-kinase binding; receptor signaling protein tyrosine kinase activity; insulin-like growth factor II binding; ATP binding; insulin receptor activity. Biological Process: heart morphogenesis; epidermis development; peptidyl-tyrosine phosphorylation; positive regulation of nitric oxide biosynthetic process; activation of MAPK activity; protein amino acid autophosphorylation; regulation of embryonic development; positive regulation of glycogen biosynthetic process; exocrine pancreas development; glucose homeostasis; positive regulation of glucose import; positive regulation of MAPKKK cascade; regulation of transcription, DNA-dependent; male sex determination; positive regulation of cell proliferation; protein heterotetramerization; positive regulation of developmental growth; positive regulation of mitosis; activation of protein kinase B; G-protein coupled receptor protein signaling pathway; positive regulation of protein kinase B signaling cascade; cellular response to insulin stimulus; carbohydrate metabolic process; positive regulation of glycolysis; insulin receptor signaling pathway; activation of protein kinase activity; positive regulation of protein amino acid phosphorylation; positive regulation of DNA replication; positive regulation of cell migration; transformation of host cell by virus. Disease: Diabetes Mellitus, Insulin-resistant, With Acanthosis Nigricans; Hyperinsulinemic Hypoglycemia, Familial, 5; Pineal Hyperplasia, Insulin-resistant Diabetes Mellitus, And Somatic Abnormalities; Donohue Syndrome

0.5毫升

725美元