抗体

5'-Nucleotidase, CT (CD73, CD73 Antigen, Ecto-5'nucleotidase, 5'-NT, eN, eNT, NT5, NT5E, NTE, Purine 5 Prime Nucleotidase)

MBS6006096

0.05毫克

695美元

多克隆

兔

未共轭

人类, 大鼠

免疫印迹, 酶联免疫吸附测定, 免疫组化, 酶免疫法

抗体

5'-Nucleotidase, CT (CD73, CD73 Antigen, Ecto-5'nucleotidase, 5'-NT, eN, eNT, NT5, NT5E, NTE, Purine 5 Prime Nucleotidase)

[5'核苷酸酶]

[Anti -5'-Nucleotidase, CT (CD73, CD73 Antigen, Ecto-5'nucleotidase, 5'-NT, eN, eNT, NT5, NT5E, NTE, Purine 5 Prime Nucleotidase)]

[5'-nucleotidase; 5'-nucleotidase; 5'-nucleotidase; 5'-NT; ecto-5'-nucleotidase; 5' nucleotidase, ecto; Ecto-5'-nucleotidase]

[Nt5e; Nt5e; Nt5; CD73; Nt5; Nte; 5'-NT]

5NTD_RAT

多克隆

IgG

兔

人类

Recognizes human NT5E. Species sequence homology: Mouse.

Affinity Purified. Purified by Protein G affinity chromatography.

以溶解在PBS液体方式提供, 0.09% 叠氮化钠

0.25毫克/毫升

May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

酶联免疫吸附测定(EL/EIA), 免疫印迹(免疫印迹), 免疫组化(免疫组化)

Suitable for use in Western Blot, ELISA and Immunohistochemistry. Dilution: ELISA: 1:1000. Western Blot: 1:100-1:500. Immunohistochemistry (Formalin fixed paraffin embedded): 10ug/ml

试验数据

免疫印迹(免疫印迹)

Immunogen: Synthetic peptide corresponding to human NT5E at C-terminal (KLH).

Antibodies; Abs to Enzymes, Nucleotidase

CD73 is a 70kD glycophosphatidylinositol (GPI)-linked 5'-nucleotidase, known as ecto-5'-nucleotidase. It converts adenosine monophosphate (AMP) to adenosine. CD73 is expressed on subsets of T- and B-cells; mesenchymal stem cells, follicular dendritic cells, endothelial cells, and epithelial cells. It has been reported that CD73 co-stimulates T cell activation, and mediates adhesion of lymphocytes to follicular dendritic cells and endothelial cells.

148747417

NP_067587.2

NM_021576.2

P21588

63,969 Da

Metabolism Pathway (828668); Metabolism Of Nucleotides Pathway (828815); Nicotinate And Nicotinamide Metabolism Pathway (83406); Nicotinate And Nicotinamide Metabolism Pathway (400); Purine Catabolism Pathway (828819); Purine Metabolism Pathway (83337); Purine Metabolism Pathway (828816); Purine Metabolism Pathway (307); Pyrimidine Catabolism Pathway (828823); Pyrimidine Metabolism Pathway (83339)

may hydrolyze extracellular nucleotides into membrane permeable nucleosides [RGD, Feb 2006]

NT5E: Hydrolyzes extracellular nucleotides into membrane permeable nucleosides. Homodimer; disulfide-linked. Belongs to the 5 -nucleotidase family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Cofactor and Vitamin Metabolism - nicotinate and nicotinamide; Phosphatase (non-protein); Membrane protein, integral; Nucleotide Metabolism - pyrimidine; Membrane protein, GPI anchor; EC 3.1.3.5; Nucleotide Metabolism - purine. Cellular Component: cell surface; membrane; cytoplasm; plasma membrane; integral to membrane. Molecular Function: 5 -nucleotidase activity; metal ion binding; ferrous iron binding; nucleotide binding. Biological Process: dephosphorylation; negative regulation of inflammatory response; adenosine biosynthetic process; positive regulation of lipid biosynthetic process; brain development; purine nucleotide biosynthetic process; adenosine metabolic process; AMP catabolic process; response to aluminum ion

0.05毫克

695美元