ELISA/assay

大鼠PDGF-C(血小板衍生生长因子C)酶联免疫吸附试剂盒

MBS2503234

24-Strip-Wells

215美元

酶联免疫吸附试剂盒

大鼠PDGF-C(血小板衍生生长因子C)酶联免疫吸附试剂盒

[PDGF-C]

[platelet-derived growth factor C; Platelet-derived growth factor C; platelet-derived growth factor C; PDGF-C; VEGF-E; secretory growth factor-like protein; spinal cord-derived growth factor; platelet derived growth factor C; Fallotein; Spinal cord-derived growth factor; SCDGF; VEGF-ECleaved into the following 2 chains:Platelet-derived growth factor C, latent form; PDGFC latent form; Platelet-derived growth factor C, receptor-binding form; PDGFC receptor-binding form]

[PDGF-C]

[PDGFC; PDGFC; SCDGF; FALLOTEIN; SCDGF; PDGF-C; SCDGF; PDGFC latent form; PDGFC receptor-binding form]

PDGFC_HUMAN

大鼠

345

This assay has high sensitivity and excellent specificity for detection of Rat PDGF-C. No significant cross-reactivity or interference between Rat PDGF-C and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Rat PDGF-C and all the analogues, therefore, cross reaction may still exist.

Store at 4 degree C.

Typical Testing Data/Standard Curve (for reference only)

Samples: Serum, Plasma, Biological Fluids. Assay Type: Sandwich. Detection Range: 15.625-1000pg/mL. Sensitivity: Min: 9.375pg/mL; Max: 1000pg/mL

Description: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of PDGF-C in Rat serum, plasma and other biological fluids. Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat PDGF-C. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Rat PDGF-C and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat PDGF-C, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Rat PDGF-C. You can calculate the concentration of Rat PDGF-C in the samples by comparing the OD of the samples to the standard curve.

9994187

NP_057289.1

NM_016205.2

Q9NRA1

20,609 Da

Choline Metabolism In Cancer Pathway (1059539); Choline Metabolism In Cancer Pathway (1084232); Cytokine-cytokine Receptor Interaction Pathway (83051); Cytokine-cytokine Receptor Interaction Pathway (460); Focal Adhesion Pathway (198795); Focal Adhesion Pathway (83067); Focal Adhesion Pathway (478); Gap Junction Pathway (83072); Gap Junction Pathway (483); Melanoma Pathway (83114)

The protein encoded by this gene is a member of the platelet-derived growth factor family. The four members of this family are mitogenic factors for cells of mesenchymal origin and are characterized by a core motif of eight cysteines. This gene product appears to form only homodimers. It differs from the platelet-derived growth factor alpha and beta polypeptides in having an unusual N-terminal domain, the CUB domain. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Sep 2010]

PDGFC: Growth factor that plays an essential role in the regulation of embryonic development, cell proliferation, cell migration, survival and chemotaxis. Potent mitogen and chemoattractant for cells of mesenchymal origin. Required for normal skeleton formation during embryonic development, especially for normal development of the craniofacial skeleton and for normal development of the palate. Required for normal skin morphogenesis during embryonic development. Plays an important role in wound healing, where it appears to be involved in three stages: inflammation, proliferation and remodeling. Plays an important role in angiogenesis and blood vessel development. Involved in fibrotic processes, in which transformation of interstitial fibroblasts into myofibroblasts plus collagen deposition occurs. The CUB domain has mitogenic activity in coronary artery smooth muscle cells, suggesting a role beyond the maintenance of the latency of the PDGF domain. In the nucleus, PDGFC seems to have additional function. Belongs to the PDGF/VEGF growth factor family. 3 isoforms of the human protein are produced by alternative splicing. Protein type: Secreted, signal peptide; Secreted. Chromosomal Location of Human Ortholog: 4q32. Cellular Component: Golgi membrane; extracellular space; cell surface; endoplasmic reticulum lumen; cytoplasm; plasma membrane; extracellular region; nucleus. Molecular Function: protein binding; protein homodimerization activity; growth factor activity; platelet-derived growth factor receptor binding. Biological Process: embryonic development; organ morphogenesis; positive regulation of fibroblast proliferation; transmembrane receptor protein tyrosine kinase activation (dimerization); central nervous system development; positive regulation of cell division; platelet-derived growth factor receptor signaling pathway; regulation of peptidyl-tyrosine phosphorylation; positive regulation of DNA replication

24-Strip-Wells

215美元

48-Strip-Wells

410

96-Strip-Wells

490

5x96-Strip-Wells

2040

10x96-Strip-Wells

3590