抗体

APC-Linked Monoclonal Antibody to Receptor Activator Of Nuclear Factor Kappa B Ligand (RANkL)

MBS2048741

0.1毫克

245美元

单克隆

小鼠

APC

免疫印迹, 免疫组化, 免疫细胞化学

抗体

APC-Linked Monoclonal Antibody to Receptor Activator Of Nuclear Factor Kappa B Ligand (RANkL)

[Receptor Activator Of Nuclear Factor Kappa B Ligand (RANkL)]

[receptor activator of nuclear factor kappa B ligand 1; Tumor necrosis factor ligand superfamily member 11; tumor necrosis factor ligand superfamily member 11; tumor necrosis factor superfamily member 11; Osteoclast differentiation factor; ODF]

[RANkL]

[TNFSF11; TNFSF11; ODF; OPGL; sOdf; CD254; OPTB2; RANKL; TNLG6B; TRANCE; hRANKL2; OPGL; RANKL; TRANCE; ODF; OPGL; RANKL; TRANCE]

单克隆

小鼠

蛋白质A/G亲和色谱

Supplied as solution form in 0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.

0.39毫克/毫升

Storage: Avoid repeated freeze/thaw cycles. Store at 4°C for frequent use. Aliquot and store at -20°C for 12 months. Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

免疫印迹(免疫印迹), 免疫组化(免疫组化), 免疫细胞化学(免疫细胞化学), 免疫荧光(IF)

Western blotting: 0.5-2 ug/mL;1:200-80. Immunohistochemistry: 5-20 ug/mL;1:20-80. Immunocytochemistry: 5-20 ug/mL;1:20-80. Optimal working dilutions must be determined by end user.

Organism Species: Rattus norvegicus (Rat). Source: Monoclonal antibody preparation. Label: APC. Traits: Liquid. Immunogen: Recombinant RANkL (Glu106~Met240) expressed in E.coli

18143619

BAB79694.1

Cytokine-cytokine Receptor Interaction Pathway (83051); Cytokine-cytokine Receptor Interaction Pathway (460); IL6-mediated Signaling Events Pathway (137932); NF-kappa B Signaling Pathway (634527); Osteoblast Signaling Pathway (198842); Osteoclast Signaling Pathway (198769); Osteoclast Differentiation Pathway (193147); Osteoclast Differentiation Pathway (193096); Prolactin Signaling Pathway (814182); Prolactin Signaling Pathway (814248)

This gene encodes a member of the tumor necrosis factor (TNF) cytokine family which is a ligand for osteoprotegerin and functions as a key factor for osteoclast differentiation and activation. This protein was shown to be a dentritic cell survival factor and is involved in the regulation of T cell-dependent immune response. T cell activation was reported to induce expression of this gene and lead to an increase of osteoclastogenesis and bone loss. This protein was shown to activate antiapoptotic kinase AKT/PKB through a signaling complex involving SRC kinase and tumor necrosis factor receptor-associated factor (TRAF) 6, which indicated this protein may have a role in the regulation of cell apoptosis. Targeted disruption of the related gene in mice led to severe osteopetrosis and a lack of osteoclasts. The deficient mice exhibited defects in early differentiation of T and B lymphocytes, and failed to form lobulo-alveolar mammary structures during pregnancy. Two alternatively spliced transcript variants have been found. [provided by RefSeq, Jul 2008]

TNFSF11: Cytokine that binds to TNFRSF11B/OPG and to TNFRSF11A/RANK. Osteoclast differentiation and activation factor. Augments the ability of dendritic cells to stimulate naive T-cell proliferation. May be an important regulator of interactions between T-cells and dendritic cells and may play a role in the regulation of the T-cell-dependent immune response. May also play an important role in enhanced bone-resorption in humoral hypercalcemia of malignancy. Homotrimer. Up-regulated by T-cell receptor stimulation. Highest in the peripheral lymph nodes, weak in spleen, peripheral blood Leukocytes, bone marrow, heart, placenta, skeletal muscle, stomach and thyroid. Belongs to the tumor necrosis factor family. 3 isoforms of the human protein are produced by alternative splicing. Protein type: Membrane protein, integral. Chromosomal Location of Human Ortholog: 13q14.11. Cellular Component: extracellular region; plasma membrane. Molecular Function: cytokine activity; tumor necrosis factor receptor superfamily binding. Biological Process: activation of NF-kappaB transcription factor; calcium-mediated signaling; cytokine and chemokine mediated signaling pathway; monocyte chemotaxis; osteoclast differentiation; positive regulation of bone resorption; positive regulation of corticotropin-releasing hormone secretion; positive regulation of homotypic cell-cell adhesion; positive regulation of MAP kinase activity; positive regulation of osteoclast differentiation; positive regulation of T cell activation; positive regulation of transcription factor activity; positive regulation of transcription from RNA polymerase II promoter; tumor necrosis factor-mediated signaling pathway. Disease: Osteopetrosis, Autosomal Recessive 2

0.1毫克

245美元

0.2毫克

330

1毫克

780