蛋白

PAPSS1, 24-624aa, 人类, 多聚组氨酸标签, 大肠杆菌

MBS204296

0.1毫克

375美元

重组蛋白

PAPSS1, 24-624aa, 人类, 多聚组氨酸标签, 大肠杆菌

PAPSS1

Bifunctional 3'-phosphoadenosine 5'-phosphosulfate synthase 1; ATPSK1; PAPSS; SK1

bifunctional 3'-phosphoadenosine 5'-phosphosulfate synthase 1; Bifunctional 3'-phosphoadenosine 5'-phosphosulfate synthase 1; bifunctional 3'-phosphoadenosine 5'-phosphosulfate synthase 1; 3-prime-phosphoadenosine 5-prime-phosphosulfate synthase 1; PAPS synthase 1; PAPSS 1; SK 1; sulfurylase kinase 1; 3'-phosphoadenosine 5'-phosphosulfate synthase 1; Sulfurylase kinase 1; SK 1; SK1

PAPSS1

PAPSS1; PAPSS1; SK1; PAPSS; ATPSK1; ATPSK1; PAPSS; PAPS synthase 1; PAPSS 1; SK 1; SK1; SAT

PAPS1_HUMAN

624

MGSSHHHHHH SSGLVPRGSH MGSHMRATNV TYQAHHVSRN KRGQVVGTRG GFRGCTVWLT GLSGAGKTTV SMALEEYLVC HGIPCYTLDG DNIRQGLNKN LGFSPEDREE NVRRIAEVAK LFADAGLVCI TSFISPYTQD RNNARQIHEG ASLPFFEVFV DAPLHVCEQR DVKGLYKKAR AGEIKGFTGI DSEYEKPEAP ELVLKTDSCD VNDCVQQVVE LLQERDIVPV DASYEVKELY VPENKLHLAK TDAETLPALK INKVDMQWVQ VLAEGWATPL NGFMREREYL QCLHFDCLLD GGVINLSVPI VLTATHEDKE RLDGCTAFAL MYEGRRVAIL RNPEFFEHRK EERCARQWGT TCKNHPYIKM VMEQGDWLIG GDLQVLDRVY WNDGLDQYRL TPTELKQKFK DMNADAVFAF QLRNPVHNGH ALLMQDTHKQ LLERGYRRPV LLLHPLGGWT KDDDVPLMWR MKQHAAVLEE GVLNPETTVV AIFPSPMMYA GPTEVQWHCR ARMVAGANFY IVGRDPAGMP HPETGKDLYE PSHGAKVLTM APGLITLEIV PFRVAAYNKK KKRMDYYDSE HHEDFEFISG TRMRKLAREG QKPPEGFMAP KAWTVLTEYY KSLEKA

> 85% by SDS - PAGE

液体。 20mM三羟甲基氨基甲烷氯化氢缓冲液(pH8.0)含20% 甘油, 0.1M氯化钠

0.5毫克/毫升(由布拉德福德Bradford检测确定)

Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.

十二烷基磺酸钠-PAGE

Antigen Species: Human. Tag: His-tag

Expression System: E Coli

酶&蛋白酶

PAPSS1, also known as bifunctional 3'-phosphoadenosine 5'-phosphosulfate synthase 1, is a bifunctional enzyme with both ATP sulfurylase and APS kinase activity, which mediates two steps in the sulfate activation pathway, yielding 3'-phosphoadenylylsulfate (PAPS). PAPSS1 is also involved in the biosynthesis of sulfated L-selectin ligands in endothelial cells. Recombinant human PAPSS1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.

Girard J.-P., et al. (1998) FASEB J. 12:603-612. Venkatachalam K.V., et al. (1999) J. Biol. Chem. 274:2601-2604

46094058

NP_005434

NM_005443.4

70.9 kDa (626aa)

Biological Oxidations Pathway (105698); Cytosolic Sulfonation Of Small Molecules Pathway (105724); Defective AHCY Causes Hypermethioninemia With S-adenosylhomocysteine Hydrolase Deficiency (HMAHCHD) Pathway (1127639); Defective B3GAT3 Causes JDSSDHD Pathway (1127588); Defective B4GALT1 Causes B4GALT1-CDG (CDG-2d) Pathway (1127592); Defective B4GALT7 Causes EDS, Progeroid Type Pathway (1127587); Defective CHST14 Causes EDS, Musculocontractural Type Pathway (1127591); Defective CHST3 Causes SEDCJD Pathway (1127590); Defective CHST6 Causes MCDC1 Pathway (1127593); Defective CHSY1 Causes TPBS Pathway (1127589)

Three-prime-phosphoadenosine 5-prime-phosphosulfate (PAPS) is the sulfate donor cosubstrate for all sulfotransferase (SULT) enzymes (Xu et al., 2000 [PubMed 10679223]). SULTs catalyze the sulfate conjugation of many endogenous and exogenous compounds, including drugs and other xenobiotics. In humans, PAPS is synthesized from adenosine 5-prime triphosphate (ATP) and inorganic sulfate by 2 isoforms, PAPSS1 and PAPSS2 (MIM 603005).[supplied by OMIM, Mar 2008]

PAPSS1: Bifunctional enzyme with both ATP sulfurylase and APS kinase activity, which mediates two steps in the sulfate activation pathway. The first step is the transfer of a sulfate group to ATP to yield adenosine 5 -phosphosulfate (APS), and the second step is the transfer of a phosphate group from ATP to APS yielding 3 -phosphoadenylylsulfate (PAPS: activated sulfate donor used by sulfotransferase). In mammals, PAPS is the sole source of sulfate; APS appears to be only an intermediate in the sulfate- activation pathway. Also involved in the biosynthesis of sulfated L-selectin ligands in endothelial cells. Protein type: Energy Metabolism - sulfur; Nucleotide Metabolism - purine; EC 2.7.1.25; EC 2.7.7.4; Other Amino Acids Metabolism - selenoamino acid; Kinase, other. Chromosomal Location of Human Ortholog: 4q24. Cellular Component: cytosol. Molecular Function: adenylylsulfate kinase activity; sulfate adenylyltransferase (ATP) activity; ATP binding; nucleotidyltransferase activity. Biological Process: sulfate assimilation; glycosaminoglycan metabolic process; xenobiotic metabolic process; carbohydrate metabolic process; pathogenesis; phosphorylation; skeletal development; 3 -phosphoadenosine 5 -phosphosulfate metabolic process; 3 -phosphoadenosine 5 -phosphosulfate biosynthetic process

0.1毫克

375美元

0.5毫克

925