蛋白

ADK, 22-362aa, 人类, 多聚组氨酸标签, 大肠杆菌

MBS203486

0.05毫克

310美元

重组蛋白

ADK, 22-362aa, 人类, 多聚组氨酸标签, 大肠杆菌

ADK

ADK;AK;腺苷激酶;腺苷5'磷酸转移酶类

adenosine kinase isoform b; Adenosine kinase; adenosine kinase; adenosine 5'-phosphotransferase; adenosine kinase; Adenosine 5'-phosphotransferase

ADK

ADK;ADK;AK;AK

ADK_HUMAN

362

MGSSHHHHHH SSGLVPRGSH MRENILFGMG NPLLDISAVV DKDFLDKYSL KPNDQILAED KHKELFDELV KKFKVEYHAG GSTQNSIKVA QWMIQQPHKA ATFFGCIGID KFGEILKRKA AEAHVDAHYY EQNEQPTGTC AACITGDNRS LIANLAAANC YKKEKHLDLE KNWMLVEKAR VCYIAGFFLT VSPESVLKVA HHASENNRIF TLNLSAPFIS QFYKESLMKV MPYVDILFGN ETEAATFARE QGFETKDIKE IAKKTQALPK MNSKRQRIVI FTQGRDDTIM ATESEVTAFA VLDQDQKEII DTNGAGDAFV GGFLSQLVSD KPLTECIRAG HYAASIIIRR TGCTFPEKPD FH

> 95% by SDS - PAGE

液体。 在20mM三羟甲基氨基甲烷氯化氢缓冲液(pH值8.0)含1mM DTT, 20% 甘油, 1mM乙二胺四乙酸, 50mM氯化钠

0.5毫克/毫升(由布拉德福德Bradford检测确定)

Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.

十二烷基磺酸钠-PAGE

Antigen Species: Human. Tag: His-tag

Expression System: E Coli

Cancer

Adenosine kinase, also known as ADK is an abundant enzyme in mammalian tissues that catalyzes the transfer of the gamma-phosphate from ATP to adenosine, thereby serving as a regulator of concentrations of both extracellular adenosine and intracellular adenine nucleotides. Adenosine kinase has widespread effects on the cardiovascular, nervous, respiratory, and immune systems and inhibitors of the enzyme could play an important pharmacological role in increasing intravascular adenosine concentrations and acting as anti-inflammatory agents. Recombinant human Adenosine kinase, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Chakraborty, A., et al. (2002) J. Biol. Chem. 277:47451-47451. Sakowicz M., et al. (2001) Acta Biochim Pol. 48(3): 745-54.

32484975

NP_006712

NM_006721.3

40.5 kDa (362aa), confirmed by MALDI-TOF

Metabolic Pathways (132956); Metabolism Pathway (477135); Metabolism Of Nucleotides Pathway (106263); Purine Metabolism Pathway (82944); Purine Metabolism Pathway (106265); Purine Metabolism Pathway (307); Purine Salvage Pathway (106273); Adenine And Adenosine Salvage II Pathway (547496); Adenine And Adenosine Salvage II Pathway (545491); Superpathway Of Purine Nucleotide Salvage (907942)

This gene an enzyme which catalyzes the transfer of the gamma-phosphate from ATP to adenosine, thereby serving as a regulator of concentrations of both extracellular adenosine and intracellular adenine nucleotides. Adenosine has widespread effects on the cardiovascular, nervous, respiratory, and immune systems and inhibitors of the enzyme could play an important pharmacological role in increasing intravascular adenosine concentrations and acting as anti-inflammatory agents. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2011]

ADK: ATP dependent phosphorylation of adenosine and other related nucleoside analogs to monophosphate derivatives. Serves as a potential regulator of concentrations of extracellular adenosine and intracellular adenine nucleotides. Defects in ADK are the cause of hypermethioninemia due to adenosine kinase deficiency (HMAKD). A metabolic disorder characterized by global developmental delay, early-onset seizures, mild dysmorphic features, and characteristic biochemical anomalies, including persistent hypermethioninemia with increased levels of S-adenosylmethionine and S-adenosylhomocysteine. Homocysteine levels are typically normal. Belongs to the carbohydrate kinase PfkB family. 3 isoforms of the human protein are produced by alternative splicing. Protein type: EC 2.7.1.20; Kinase, other; Nucleotide Metabolism - purine. Chromosomal Location of Human Ortholog: 10q22 10q11-q24. Cellular Component: nucleoplasm; cytoplasm; cytosol. Molecular Function: metal ion binding; adenosine kinase activity; phosphotransferase activity, alcohol group as acceptor; ATP binding. Biological Process: nucleobase, nucleoside and nucleotide metabolic process; dATP biosynthetic process; ribonucleoside monophosphate biosynthetic process; positive regulation of T cell proliferation; circadian regulation of gene expression; adenosine metabolic process; phosphorylation; purine salvage; purine base metabolic process. Disease: Hypermethioninemia Due To Adenosine Kinase Deficiency

0.05毫克

310美元

0.25毫克

720