产品简要
公司名称 :
MyBioSource
产品类型 :
蛋白
产品名称 :
FEN1, 1-380aa, 人类, 大肠杆菌
目录 :
MBS203445
规格 :
0.1毫克
价格 :
375美元
更多信息或购买 :
产品信息
目录号 :
MBS203445
产品类型 :
重组蛋白
产品全称 :
FEN1, 1-380aa, 人类, 大肠杆菌
产品简称 :
FEN1
产品名称同义词 :
FEN-1; MF1; RAD2; Flap structure-specific endonuclease 1 DNase IV; Flap endonuclease 1; Flap structure specific endonuclease 1; hFEN1; Maturation factor 1
其他名称 :
flap endonuclease 1; Flap endonuclease 1; flap endonuclease 1; DNase IV; FEN-1; FEN1; maturation factor 1; maturation factor-1; flap structure-specific endonuclease 1; DNase IVFlap structure-specific endonuclease 1
产品基因名称 :
FEN1
其他基因名称 :
FEN1;FEN1;MF1;RAD2;FEN-1;
UniProt数据库进入名 :
FEN1_HUMAN
序列长度 :
380
序列 :
MGIQGLAKLI ADVAPSAIRE NDIKSYFGRK VAIDASMSIY QFLIAVRQGG DVLQNEEGET TSHLMGMFYR TIRMMENGIK PVYVFDGKPP QLKSGELAKR SERRAEAEKQ LQQAQAAGAE QEVEKFTKRL VKVTKQHNDE CKHLLSLMGI PYLDAPSEAE ASCAALVKAG KVYAAATEDM DCLTFGSPVL MRHLTASEAK KLPIQEFHLS RILQELGLNQ EQFVDLCILL GSDYCESIRG IGPKRAVDLI QKHKSIEEIV RRLDPNKYPV PENWLHKEAH QLFLEPEVLD PESVELKWSE PNEEELIKFM CGEKQFSEER IRSGVKRLSK SRQGSTQGRL DDFFKVTGSL SSAKRKEPEP KGSTKKKAKT GAAGKFKRGK
纯度 :
> 90% by SDS - PAGE
形式 :
液体。 在20 mM三羟甲基氨基甲烷氯化氢缓冲液(pH8.0)含1mM DTT 10% 甘油
浓度 :
1毫克/毫升(由布拉德福德Bradford检测确定)
储存稳定性 :
Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.
检测过的应用 :
十二烷基磺酸钠-PAGE
其它信息1 :
Antigen Species: Human
其他信息2 :
Expression System: E Coli
产品种类 :
酶&蛋白酶
产品描述 :
FEN-1, also known as Flap structure-specific endonuclease 1, removes 5' overhanging flaps in DNA repair and processes the 5' ends of Okazaki fragments in lagging strand DNA synthesis. The protein is a member of the XPG/RAD2 endonuclease family and is one of ten proteins essential for cell-free DNA replication. FEN-1 is highly homologous to yeast Rad2. The C-terminal region of FEN-1 may bind to PCNA, thus allowing FEN-1 to function as an exonuclease in DNA replication. Recombinant human FEN-1 protein was expressed in E.coli and purified by using conventional chromatography techniques.
产品引用 :
Hakoyama T., et al. (2009) Nature. 462(7272):514-7. Lal A., et al. (2009) Mol Cell. 35(5):610-25.
NCBI GI登录号 :
4758356
NCBI登录号 :
NP_004102
NCBI基因登录号 :
NM_004111.5
NCBI分子量 :
42.5 kDa (380aa), confirmed by MALDI-TOF.
NCBI信号通路 :
Base Excision Repair Pathway (105838); Base Excision Repair Pathway (83043); Base Excision Repair Pathway (451); Cell Cycle Pathway (530733); Cell Cycle, Mitotic Pathway (105765); Chromosome Maintenance Pathway (161048); DNA Repair Pathway (105837); DNA Replication Pathway (105897); DNA Replication Pathway (83039); DNA Replication Pathway (444)
NCBI总结 :
The protein encoded by this gene removes 5' overhanging flaps in DNA repair and processes the 5' ends of Okazaki fragments in lagging strand DNA synthesis. Direct physical interaction between this protein and AP endonuclease 1 during long-patch base excision repair provides coordinated loading of the proteins onto the substrate, thus passing the substrate from one enzyme to another. The protein is a member of the XPG/RAD2 endonuclease family and is one of ten proteins essential for cell-free DNA replication. DNA secondary structure can inhibit flap processing at certain trinucleotide repeats in a length-dependent manner by concealing the 5' end of the flap that is necessary for both binding and cleavage by the protein encoded by this gene. Therefore, secondary structure can deter the protective function of this protein, leading to site-specific trinucleotide expansions. [provided by RefSeq, Jul 2008]
UniProt数据库总结 :
FEN1: Structure-specific nuclease with 5 -flap endonuclease and 5 -3 exonuclease activities involved in DNA replication and repair. During DNA replication, cleaves the 5 -overhanging flap structure that is generated by displacement synthesis when DNA polymerase encounters the 5 -end of a downstream Okazaki fragment. It enters the flap from the 5 -end and then tracks to cleave the flap base, leaving a nick for ligation. Also involved in the long patch base excision repair (LP-BER) pathway, by cleaving within the apurinic/apyrimidinic (AP) site-terminated flap. Acts as a genome stabilization factor that prevents flaps from equilibrating into structurs that lead to duplications and deletions. Also possesses 5 -3 exonuclease activity on nicked or gapped double- stranded DNA, and exhibits RNase H activity. Also involved in replication and repair of rDNA and in repairing mitochondrial DNA. Interacts with PCNA. Three molecules of FEN1 bind to one PCNA trimer with each molecule binding to one PCNA monomer. PCNA stimulates the nuclease activity without altering cleavage specificity. The C-terminal domain binds EP300. Can bind simultaneously to both PCNA and EP300. Interacts with DDX11. Belongs to the XPG/RAD2 endonuclease family. FEN1 subfamily. Protein type: DNA-binding; Nucleolus; Nuclear receptor co-regulator; Ribonuclease; Deoxyribonuclease; DNA repair, damage; EC 3.1.-.-. Chromosomal Location of Human Ortholog: 11q12. Cellular Component: nucleoplasm; mitochondrion; membrane; nucleolus; plasma membrane; nucleus. Molecular Function: 5 -3 exonuclease activity; protein binding; DNA binding; 5 -flap endonuclease activity; endonuclease activity; manganese ion binding; double-stranded DNA binding; exonuclease activity; magnesium ion binding; damaged DNA binding; ribonuclease H activity; double-stranded DNA specific exodeoxyribonuclease activity. Biological Process: telomere maintenance via semi-conservative replication; DNA replication, removal of RNA primer; UV protection; base-excision repair; double-strand break repair; telomere maintenance via recombination; mitotic cell cycle; DNA strand elongation during DNA replication; DNA catabolic process, exonucleolytic; DNA repair; DNA replication; DNA catabolic process, endonucleolytic; telomere maintenance; memory
尺寸1 :
0.1毫克
价格1 :
375美元
尺寸2 :
0.5毫克
价格2 :
925
更多信息或购买 :
公司信息
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MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com1-858-633-0165
公司总部: 美国
MyBioSource,LLC最初由三名热情澎湃的提供高品质试剂的科学家联合创立于温哥华,公司愿景是“生物研究试剂的源头”,现在位于圣地亚哥市。