产品简要
公司名称 :
MyBioSource
产品类型 :
蛋白
产品名称 :
醛缩酶A, 1-364aa, 人类, 多聚组氨酸标签, 大肠杆菌
目录 :
MBS203442
规格 :
0.1毫克
价格 :
285美元
更多信息或购买 :
产品信息
目录号 :
MBS203442
产品类型 :
重组蛋白
产品全称 :
醛缩酶A, 1-364aa, 人类, 多聚组氨酸标签, 大肠杆菌
产品简称 :
醛缩酶A
产品名称同义词 :
ALDOA; ALDA; GSD12; Fructose bisphosphate aldolase A Aldolase A; Aldolase A fructose bisphosphatase; Aldolase A fructose bisphosphate; Fructose 1 6 bisphosphate triosephosphate lyase; Fructose bisphosphate aldolase; Lung cancer antigen NY Lu 1; MGC10942; MGC17716; MGC17767; Muscle type aldolase
其他名称 :
fructose-bisphosphate aldolase A isoform 1; Fructose-bisphosphate aldolase A; fructose-bisphosphate aldolase A; epididymis secretory sperm binding protein Li 87p; fructose-1,6-bisphosphate triosephosphate-lyase; lung cancer antigen NY-LU-1; muscle-type aldolase; aldolase A, fructose-bisphosphate; Lung cancer antigen NY-LU-1; Muscle-type aldolase
其他基因名称 :
ALDOA;ALDOA;ALDA;GSD12;HEL-S-87p;ALDA
UniProt数据库进入名 :
ALDOA_HUMAN
序列长度 :
364
序列 :
MGSSHHHHHH SSGLVPRGSH MPYQYPALTP EQKKELSDIA HRIVAPGKGI LAADESTGSI AKRLQSIGTE NTEENRRFYR QLLLTADDRV NPCIGGVILF HETLYQKADD GRPFPQVIKS KGGVVGIKVD KGVVPLAGTN GETTTQGLDG LSERCAQYKK DGADFAKWRC VLKIGEHTPS ALAIMENANV LARYASICQQ NGIVPIVEPE ILPDGDHDLK RCQYVTEKVL AAVYKALSDH HIYLEGTLLK PNMVTPGHAC TQKFSHEEIA MATVTALRRT VPPAVTGITF LSGGQSEEEA SINLNAINKC PLLKPWALTF SYGRALQASA LKAWGGKKEN LKAAQEEYVK RALANSLACQ GKYTPSGQAG AAASESLFVS NHAY
纯度 :
> 95% by SDS - PAGE
形式 :
液体。 在20mM三羟甲基氨基甲烷氯化氢缓冲液(pH8.0)含100mM氯化钠, 10% 甘油
浓度 :
1毫克/毫升(由布拉德福德Bradford检测确定)
储存稳定性 :
Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.
检测过的应用 :
十二烷基磺酸钠-PAGE
其它信息1 :
Antigen Species: Human. Tag: His-tag
其他信息2 :
Expression System: E Coli. Bioactivity: Specific activity is > 1.5 units/mg, one unit will convert 1.0 umole of fructose 1,6-diphosphate to dihydroxyacetone phosphate and glyceraldehyde 3-phosphate per minute at pH 7.5 at 25C. Assay: 1. Prepare a 1.45 ml reaction mixture with the following concentrations: 45 mM Tris pH8.0, 0.95mM fructose 1,6-diphosphate, 0.065 mM beta-nicotinamide adenine dinucleotide, 5 unit alpha-glcerophosphate dehydrogenase/triosephosphate isomerase. 2. Add 50 ul of recombinant Aldolase A protein with 1ug, 2ug in reaction mixture. 3. Record the decrease in A340nm for 5 minutes at 25C.
产品种类 :
代谢
产品描述 :
Fructose bisphosphate aldolase A, also known as Aldolase A is a glycolytic enzyme that catalyzes the reversible conversion of fructose-1,6-bisphosphate to glyceraldehyde 3-phosphate and dihydroxyacetone phosphate. It is found in the developing embryo and is produced in even greater amounts in adult muscle. Aldolase A expression is repressed in adult liver, kidney and intestine and similar to aldolase C levels in brain and other nervous tissue. Deficiency has been associated with myopathy and hemolytic anemia. Recombinant human Aldolase A, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
产品引用 :
Pfleiderer G., et al. (1975) Beitr Pathol. 156(3):266-79. Kishi H., et al. (1987) Proc Natl Acad uSA. 84(23):8623-7.
NCBI GI登录号 :
34577110
NCBI登录号 :
NP_908930
NCBI基因登录号 :
NM_184041.2
NCBI分子量 :
41.5 kDa (384aa), confirmed by MALDI-TOF.
NCBI信号通路 :
Biosynthesis Of Amino Acids Pathway (790012); Biosynthesis Of Amino Acids Pathway (795174); Carbon Metabolism Pathway (814926); Carbon Metabolism Pathway (817567); Disease Pathway (530764); Fructose And Mannose Metabolism Pathway (82930); Fructose And Mannose Metabolism Pathway (291); Gluconeogenesis Pathway (106204); Gluconeogenesis, Oxaloacetate = Fructose-6P Pathway (413342); Gluconeogenesis, Oxaloacetate = Fructose-6P Pathway (468196)
NCBI总结 :
The protein encoded by this gene, Aldolase A (fructose-bisphosphate aldolase), is a glycolytic enzyme that catalyzes the reversible conversion of fructose-1,6-bisphosphate to glyceraldehyde 3-phosphate and dihydroxyacetone phosphate. Three aldolase isozymes (A, B, and C), encoded by three different genes, are differentially expressed during development. Aldolase A is found in the developing embryo and is produced in even greater amounts in adult muscle. Aldolase A expression is repressed in adult liver, kidney and intestine and similar to aldolase C levels in brain and other nervous tissue. Aldolase A deficiency has been associated with myopathy and hemolytic anemia. Alternative splicing and alternative promoter usage results in multiple transcript variants. Related pseudogenes have been identified on chromosomes 3 and 10. [provided by RefSeq, Aug 2011]
UniProt数据库总结 :
ALDOA: a glycolytic enzyme that catalyzes D-fructose 1,6-bisphosphate - glycerone phosphate + D-glyceraldehyde 3-phosphate. Three forms of aldolase are found in vertebrates - aldolase A in muscle, aldolase B in liver and aldolase C in brain. Protein type: Carbohydrate Metabolism - glycolysis and gluconeogenesis; EC 4.1.2.13; Carbohydrate Metabolism - fructose and mannose; Lyase; Mitochondrial; Carbohydrate Metabolism - pentose phosphate pathway. Chromosomal Location of Human Ortholog: 16p11.2. Cellular Component: I band; extracellular space; membrane; extracellular region; M band; cytosol; nucleus; actin cytoskeleton. Molecular Function: tubulin binding; identical protein binding; protein binding; cytoskeletal protein binding; fructose-bisphosphate aldolase activity; actin binding. Biological Process: platelet activation; striated muscle contraction; glycolysis; actin filament organization; glucose metabolic process; pathogenesis; gluconeogenesis; protein homotetramerization; muscle maintenance; fructose 1,6-bisphosphate metabolic process; regulation of cell shape; platelet degranulation; ATP biosynthetic process; carbohydrate metabolic process; blood coagulation; fructose metabolic process. Disease: Glycogen Storage Disease Xii
尺寸1 :
0.1毫克
价格1 :
285美元
尺寸2 :
0.5毫克
价格2 :
640
更多信息或购买 :
公司信息
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MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com1-858-633-0165
公司总部: 美国
MyBioSource,LLC最初由三名热情澎湃的提供高品质试剂的科学家联合创立于温哥华,公司愿景是“生物研究试剂的源头”,现在位于圣地亚哥市。
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